SCID Mouse: Human Xenograft Core

SCID 小鼠：人类异种移植核心

• 批准号: 7608570
• 负责人: JORDAN S POBER
• 金额: $ 11.28万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7608570
• 关键词: Adult; Allogenic; Antibodies; Autologous; B-Lymphocytes; Biopsy; Blood Cells; Cells; Development; Elements; Endothelium; Engraftment; Family suidae; Future; Hematopoietic System; Human; Immunodeficient Mouse; Infectious Agent; Infectious Skin Diseases; Inflammatory; Life; Methods; Modeling; Mus; Mutant Strains Mice; Neonatal; Numbers; Patients; Population; Preparation; Research Personnel; SCID Mice; Skin; Skin graft; Sus scrofa; T-Lymphocyte; Techniques; Technology; Thick; Time; Training; Variant; Work; Xenograft Model; Xenograft procedure; in vivo; member; neutrophil; novel; penis foreskin; research study; response; skin disorder; skin xenograft; vascular bed; volunteer

The SCID mouse: The skin xenograft core has developed methods to routinely generate severe combined immunodeficient (SCID) beige (bg) double mutant mice engrafted with human skin in the form of split thickness skin grafts or with synthetic skin constructs incorporating human skin cells. The three specific aims of the core are (1) to provide members of the YSDRC (both established and junior investigators, and especially investigators with YSDRC P/F projects) with limited numbers of C.B-17 SCID/beige mice engrafted with healthy adult human skin or skin constructs for establishing the utility of these models for specific skin disease-related studies; (2) to train members of the YSDRC to generate such mice once feasibility has been established; and (3) to develop variant models of this technique for new and novel applications, specifically focusing on synthetic skin equivalents constructed from distinct populations of cells both from patients with specific skin diseases and/or that may be genetically modified to alter biologic functions. The core will also continue evaluating alternative mouse recipient strains and methods to combine human skin grafts with elements of the human hematopoietic system to better model inflammatory skin disorders and responses to skin infections.

SCID小鼠：皮肤异种移植核心已经开发出常规产生严重联合移植物的方法。 免疫缺陷（SCID）米色（bg）双突变小鼠，以分层厚度的形式移植人皮肤 皮肤移植物或与掺入人皮肤细胞的合成皮肤构建物结合。三个具体目标 核心是（1）提供YSDRC的成员（包括已建立的和初级调查员，特别是 YSDRC P/F项目的研究者），有限数量的C.B-17 SCID/beige小鼠移植有 用于建立这些模型对特定皮肤的效用的健康成人皮肤或皮肤构建体 疾病相关的研究;（2）培训YSDRC的成员，一旦可行，就生产这样的小鼠。 建立;和（3）为新的和新颖的应用开发这种技术的变体模型，特别是 专注于由不同细胞群构建的合成皮肤等同物， 特定的皮肤疾病和/或可以被遗传修饰以改变生物功能。核心还将 继续评估替代小鼠受体品系和方法，以将联合收割机人类皮肤移植物与 人类造血系统的元素，以更好地模拟炎症性皮肤疾病和对 皮肤感染