SCID Mouse: Human Xenograft Core

SCID小鼠：异种皮肤移植核心已经开发出常规方法来产生严重的联合 免疫缺陷(SCID)米色(BG)双突变小鼠，以劈裂厚度的形式植入人皮肤 皮肤移植或含有人类皮肤细胞的合成皮肤结构。的三个具体目标 核心是：(1)向YSDRC的成员(包括常设和初级调查人员，特别是 YSDRC P/F项目的调查人员)将有限数量的C.B-17 SCID/Beige小鼠移植到 用于建立这些模型对特定皮肤的实用性的健康成人皮肤或皮肤构造 与疾病相关的研究；(2)一旦可行，培训YSDRC的成员生产这种小鼠 建立；以及(3)为新的和新的应用开发该技术的不同模型，特别是 关注由不同群体的细胞构建的合成皮肤等效物，这两种细胞来自于 特定的皮肤病和/或可能经过基因改造以改变生物功能的疾病。核心也将 继续评估将人类皮肤移植与人类皮肤移植相结合的替代小鼠受体品系和方法 用于更好地模拟炎症性皮肤疾病的人类造血系统的元素和对 皮肤感染。

The SCID mouse: The skin xenograft core has developed methods to routinely generate severe combined immunodeficient (SCID) beige (bg) double mutant mice engrafted with human skin in the form of split thickness skin grafts or with synthetic skin constructs incorporating human skin cells. The three specific aims of the core are (1) to provide members of the YSDRC (both established and junior investigators, and especially investigators with YSDRC P/F projects) with limited numbers of C.B-17 SCID/beige mice engrafted with healthy adult human skin or skin constructs for establishing the utility of these models for specific skin disease-related studies; (2) to train members of the YSDRC to generate such mice once feasibility has been established; and (3) to develop variant models of this technique for new and novel applications, specifically focusing on synthetic skin equivalents constructed from distinct populations of cells both from patients with specific skin diseases and/or that may be genetically modified to alter biologic functions. The core will also continue evaluating alternative mouse recipient strains and methods to combine human skin grafts with elements of the human hematopoietic system to better model inflammatory skin disorders and responses to skin infections.