Analysis of Alveolar Type II cells in Normal and Fibrotic Human Lung

正常和纤维化人肺中 II 型肺泡细胞的分析

• 批准号: 8113080
• 负责人: Paul j WOLTERS
• 金额: $ 23.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8113080
• 关键词: Accounting; Address; Alveolar; Animal Model; Apoptosis; Architecture; Biologic Characteristic; Biological Assay; Biology; Candidate Disease Gene; Cell Proliferation; Cell surface; Cells; Cellular biology; Characteristics; Collagen; Cytokeratin; Data; Deposition; Development; Diagnosis; Dinoprostone; Disease; E-Cadherin; Epithelial; Epithelial Cells; Fibroblasts; Fibrosis; Fingerprint; Future; Gene Expression; Genes; Genetic; Genomics; Growth Factor; Hamman-Rich syndrome; Human; Hyperplasia; Immunohistochemistry; Impaired wound healing; In Vitro; Interstitial Lung Diseases; Investigation; Knowledge; Lead; Lung; Lung diseases; Mediator of activation protein; Mesenchymal; Messenger RNA; Metalloproteases; Methods; MicroRNAs; Normal Cell; Outcome; Pathogenesis; Pathway interactions; Patients; Phenotype; Physiological; Play; Population; Process; Proliferating; Prostaglandins; Proteins; RNA; Research Proposals; Respiratory physiology; Reverse Transcriptase Polymerase Chain Reaction; Role; Sorting - Cell Movement; Source; Surface; Technology; Testing; Time; Type II Epithelial Receptor Cell; alveolar type II cell; cell type; endoplasmic reticulum stress; epithelial to mesenchymal transition; in vitro Assay; new technology; novel; research study; response to injury

DESCRIPTION (provided by applicant): Idiopathic pulmonary fibrosis (IPF) accounts for more than half of the cases of interstitial lung disease. Patients suffering from IPF develop a progressive loss of lung function and die, on average, 3 years after diagnosis. In addition to fibrosis, alveolar type II cells are abnormal in IPF lungs. However, their role in disease pathogenesis remains undefined. This proposal examines the novel hypothesis that epithelial cells directly participate in the formation of lung fibrosis by transitioning into collagen secreting mesenchymal cells. Within IPF lung there is a subpopulation of epithelial cells predisposed to transition into fibroblasts. These epithelial derived fibroblasts are a major source of the matrix remodeling and fibrosis that occurs in IPF lung. Specific aims pursued to address this hypothesis are: 1) To establish the unique phenotypic characteristics of alveolar type II cells purified from lungs of IPF patients. 2) To isolate and characterize an alveolar Type II cell subtype expressing CD15 found in IPF lungs. Approach: To pursue these aims, pure populations of Type II cells will be isolated from normal and IPF lungs using FACS sorting for E-cadherin. Gene expression arrays, rt-PCR, and immunohistochemistry will be used to define changes in gene expression unique to IPF Type II cells that may contribute to disease pathogenesis. The role of candidate genes that promote epithelial to mesenchymal transition (EMT) of IPF Type II cells will be confirmed in an in vitro assay of EMT. In Aim 2, FACS sorting will be used to isolate a Type II cell subtype expressing the cell surface marker CD15. This subtype represents up to 6% of Type II cells in IPF lungs. Purified isolates of CD15+ type cells will be compared to CD15- Type II cells for their ability to proliferate, undergo apoptosis, or transition to mesenchymal cells. Gene expression arrays will be used to define their unique genetic phenotype and how it relates to the pathogenesis of IPF. Significance: By completing the proposed aims, significant gaps in knowledge of the pathogenesis of IPF and basic lung biology will be resolved including; 1) The studies will provide new data that specifically phenotype differences between normal and fibrotic Type II cells. 2) They will establish that EMT occurs in IPF lungs and the mediators of this process, 3) They will establish for the first time a specific Type II cell subtype that exists within human lung and the distinct phenotypic characteristics of this phenotype, 4) In addition to advancing our understanding of the pathogenesis of lung fibrosis, the new data and technologies developed via these experiments can be used in future studies of the pathogenesis of IPF and the biology of alveolar Type II cells in human lung. PUBLIC HEALTH RELEVANCE: Idiopathic pulmonary fibrosis, the most common form of lung fibrosis, is a deadly disease with average survival of three years. This proposal applies novel technologies to purify and characterize alveolar Type II cells and Type II cell subtypes (CD15+) from the lungs of patients with idiopathic pulmonary fibrosis. Following purification, the unique biological characteristics of IPF Type II cells and CD15+ Type II cells will be established. By broadly analyzing the unique biology of IPF Type II cells, these studies have the potential to identify unsuspected causes of Idiopathic pulmonary fibrosis and could lead to the development of new therapies that favorably modulate outcomes of this devastating disease.

描述（由申请人提供）：特发性肺纤维化（IPF）占间质性肺病病例的一半以上。患有特发性肺纤维化的患者会出现肺功能进行性丧失，并平均在诊断后3年死亡。除了纤维化，肺泡II型细胞在IPF肺中也异常。然而，它们在疾病发病机制中的作用仍不明确。该提案检验了上皮细胞通过转变为分泌胶原的间充质细胞直接参与肺纤维化形成的新假设。在IPF肺内，存在倾向于转变为成纤维细胞的上皮细胞亚群。这些上皮来源的成纤维细胞是IPF肺中发生的基质重塑和纤维化的主要来源。解决这一假设的具体目标是：1）建立从IPF患者肺中纯化的肺泡II型细胞的独特表型特征。2)分离和表征IPF肺中发现的表达CD 15的肺泡II型细胞亚型。方法：为了实现这些目标，将使用E-钙粘蛋白的FACS分选从正常和IPF肺中分离纯的II型细胞群。基因表达阵列、rt-PCR和免疫组织化学将用于确定可能导致疾病发病机制的IPF II型细胞特有的基因表达变化。促进IPF II型细胞上皮向间充质转化（EMT）的候选基因的作用将在EMT的体外试验中得到证实。在目标2中，将使用FACS分选来分离表达细胞表面标志物CD 15的II型细胞亚型。该亚型占IPF肺中II型细胞的6%。将比较CD 15+型细胞的纯化分离物与CD 15-II型细胞的增殖、凋亡或向间充质细胞转化的能力。基因表达阵列将用于确定其独特的遗传表型及其与IPF发病机制的关系。重要性：通过完成提出的目标，将解决IPF发病机制和基本肺生物学知识的重大空白，包括：1）研究将提供新数据，特别是正常和纤维化II型细胞之间的表型差异。2)他们将确定EMT发生在IPF肺和该过程的介质中，3）他们将首次确定存在于人肺内的特定II型细胞亚型和该表型的独特表型特征，4）除了推进我们对肺纤维化发病机制的理解，通过这些实验开发的新数据和技术可用于未来对IPF发病机制和人肺肺泡II型细胞生物学的研究。 公共卫生关系：特发性肺纤维化是肺纤维化最常见的形式，是一种致命的疾病，平均生存期为三年。该提案应用新技术纯化和表征特发性肺纤维化患者肺部的肺泡II型细胞和II型细胞亚型（CD 15+）。纯化后，将确定IPF II型细胞和CD 15 + II型细胞的独特生物学特性。通过广泛分析IPF II型细胞的独特生物学，这些研究有可能确定特发性肺纤维化的未知原因，并可能导致新疗法的开发，从而有利地调节这种毁灭性疾病的结局。