Ankyrin G in protein sorting between rod plasma membrane and photoreceptor discs
锚蛋白 G 在杆质膜和感光盘之间的蛋白质分选中的作用
基本信息
- 批准号:8053279
- 负责人:
- 金额:$ 18.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-04-01 至 2012-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAnkyrinsAntibodiesApicalBindingBiogenesisCattleCell membraneComplexCytoskeletonDegenerative DisorderDetergentsDiseaseElementsEnsureEpithelial CellsFunctional disorderFundingFutureGoalsHealthImmunoprecipitationInheritedIntracellular MembranesInvestigationKnockout MiceLightMaintenanceMass Spectrum AnalysisMechanicsMembraneMembrane ProteinsMolecularMorphogenesisPhotoreceptorsPositioning AttributeProcessProtein BindingProteinsReporterReportingRetinaRetinitis PigmentosaRhodopsinRod Outer SegmentsRoleSecureSorting - Cell MovementTestingTherapeutic Interventioncell typecyclic-nucleotide gated ion channelsdisorder preventioninsightmutantphotoreceptor cell outer segmentphotoreceptor discpreventprogramsprotein transportpublic health relevanceresearch studyretinal rodstooltrafficking
项目摘要
DESCRIPTION (provided by applicant): The overall goal of this application is to explore the role of the membrane cytoskeleton in the functioning of photoreceptor outer segments. The membrane cytoskeleton decorates plasma membranes in essentially all cell types, providing them with mechanical support and retaining selected membrane proteins within specialized membrane sub-domains. Surprisingly, the role of the membrane cytoskeleton in photoreceptors had not been systematically addressed until very recently, when we reported that the outer segment targeting of the 2-subunit of the cyclic nucleotide-gated channel is critically dependent on its interaction with the membrane cytoskeleton protein ankyrin G. We now propose that another major function of ankyrin G is to retain this channel and other proteins at the rod outer segment plasma membrane and to prevent them from sorting into photoreceptor discs. In Aim 1 we will test this hypothesis for the case of the cyclic nucleotide-gated channel. In Aim 2 we will search for other ankyrin G binding partners in rod outer segments. We will also test whether selective plasma membrane localization of other proteins in rods is dependent on their binding to ankyrin G. We hope that the completion of this exploratory study will yield a sufficient body of information, concepts and experimental tools to build a larger program devoted to understanding the roles of the membrane cytoskeleton in photoreceptor health and disease.
PUBLIC HEALTH RELEVANCE: The studies proposed in this application address the molecular and cellular mechanisms responsible for the biogenesis, maintenance and functioning of the light-sensitive compartment of the photoreceptor cells, the outer segment. Dysfunction of these processes causes some of the most severe types of inherited degenerative diseases of the retina, including but not limited to retinitis pigmentosa. This highlights the importance of understanding the mechanisms underlying the trafficking of proteins to the outer segment and their subsequent assembly into large functional complexes. Elucidating these mechanisms is essential for developing strategies for disease prevention and future therapeutic interventions.
描述(由申请人提供):本申请的总体目标是探索膜细胞骨架在感光器外节功能中的作用。膜细胞骨架装饰基本上所有细胞类型的质膜,为它们提供机械支撑并将选定的膜蛋白保留在专门的膜子域内。令人惊讶的是,膜细胞骨架在光感受器中的作用直到最近才被系统地解决,当时我们报道了环核苷酸门控通道2-亚基的外段靶向关键取决于其与膜细胞骨架蛋白锚蛋白G的相互作用。我们现在提出锚蛋白G的另一个主要功能是保留该通道和其他蛋白 位于杆外段质膜上,并防止它们分类到感光盘中。在目标 1 中,我们将在环核苷酸门控通道的情况下测试这一假设。在目标 2 中,我们将寻找杆外节中的其他锚蛋白 G 结合伴侣。我们还将测试杆中其他蛋白质的选择性质膜定位是否依赖于它们与锚蛋白 G 的结合。我们希望这项探索性研究的完成将产生足够的信息、概念和实验工具,以建立一个更大的项目,致力于了解膜细胞骨架在光感受器健康和疾病中的作用。
公共健康相关性:本申请提出的研究涉及负责感光细胞感光区(外节)的生物发生、维持和功能的分子和细胞机制。这些过程的功能障碍会导致一些最严重类型的遗传性视网膜退行性疾病,包括但不限于色素性视网膜炎。这凸显了了解蛋白质运输到外段及其随后组装成大型功能复合物的机制的重要性。阐明这些机制对于制定疾病预防和未来治疗干预策略至关重要。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Vadim Y Arshavsky其他文献
Vadim Y Arshavsky的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Vadim Y Arshavsky', 18)}}的其他基金
Molecular mechanisms of photoreceptor disc morphogenesis
光感受器盘形态发生的分子机制
- 批准号:
10749286 - 财政年份:2023
- 资助金额:
$ 18.72万 - 项目类别:
Rhodopsin dimerization: mechanistic basis and functional consequences
视紫红质二聚化:机制基础和功能后果
- 批准号:
9301797 - 财政年份:2017
- 资助金额:
$ 18.72万 - 项目类别:
FASEB SRC on Biology and Chemistry of Vision
FASEB SRC 视觉生物学和化学
- 批准号:
8908352 - 财政年份:2015
- 资助金额:
$ 18.72万 - 项目类别:
Role of impaired protein degradation in photoreceptor degeneration
蛋白质降解受损在光感受器变性中的作用
- 批准号:
8894001 - 财政年份:2013
- 资助金额:
$ 18.72万 - 项目类别:
Role of impaired protein degradation in photoreceptor degeneration
蛋白质降解受损在光感受器变性中的作用
- 批准号:
8578034 - 财政年份:2013
- 资助金额:
$ 18.72万 - 项目类别:
Role of impaired protein degradation in photoreceptor degeneration
蛋白质降解受损在光感受器变性中的作用
- 批准号:
8705524 - 财政年份:2013
- 资助金额:
$ 18.72万 - 项目类别:
Ankyrin G in protein sorting between rod plasma membrane and photoreceptor discs
锚蛋白 G 在杆质膜和感光盘之间的蛋白质分选中的作用
- 批准号:
7869100 - 财政年份:2010
- 资助金额:
$ 18.72万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 18.72万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 18.72万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 18.72万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 18.72万 - 项目类别:
Studentship