Regulated Assembly of Subcellular Structures in alpha-Proteobacteria
α-变形菌亚细胞结构的调控组装
基本信息
- 批准号:8101439
- 负责人:
- 金额:$ 3.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:AffectAlphaproteobacteriaAutomobile DrivingBacteriaBacterial ProteinsBiogenesisBrucellaCaulobacter crescentusCell divisionCellsCellular MorphologyDevelopmentEnsureEnvironmentFabaceaeFlagellaFundingFutureGenesGoalsHomologous ProteinInfectionInstitutionLaboratoriesLocationMelilotusMentorsMentorshipMicrobeModelingMolecular StructureMutationNitrogenNoduleOrganellesOrganismPathway interactionsPatternPhenotypePilumPlant RootsPlantsPolar RegionsPositioning AttributePrincipal InvestigatorProteinsProteobacteriaPublic HealthPublicationsRegulationRegulatory PathwayResearchRickettsiaRoleScientistSenior ScientistSinorhizobium melilotiStudentsSubcellular structureSurfaceSymbiosisSystemTimeTrainingVirulence FactorsWorkantimicrobialgenetic regulatory proteinimprovedmutantnovelpathogenpost-doctoral trainingpreventprotein complexskillssuccesstransmission process
项目摘要
DESCRIPTION (provided by applicant): Bacterial cells have evolved multiple regulatory mechanisms to ensure that their subcellular structures and organelles, such as pili and flagella, are assembled and positioned correctly. Our goal is to determine how the core components of a conserved regulatory system, and their interactions, have been adapted to serve the particular needs of different organisms. In Caulobacter crescentus, the protein components of this regulatory system (PodJ, DivJ, and PleC) follow defined patterns of subcellular localization that contribute to asymmetric cell morphology: organelles specifically develop at one pole but not the other. The regulatory components are conserved in Sinorhizobium meliloti, a related bacterium that induces nodule formation in plant roots during symbiosis. However, S. meliloti cells are morphologically symmetric compared to C. crescentus. We will examine how these conserved components function in S. meliloti to regulate the assembly of surface organelles and how this regulation affects host-microbe interaction. (1) We will first determine the localization patterns of the regulatory proteins and the phenotypes of null mutants that lack the proteins. (2) We will then detect and analyze changes in the mutants' transcriptional profiles to see if the proteins control expression of similar genes in different species. (3) We will also assess whether the mutations disrupt interaction between the bacterium and its hosts, preventing effective nodulation. In addition to these research objectives, the principal investigator aims to achieve the following developmental objectives under the guidance of a senior scientist: (4) establish an independent research group, (5) enhance mentoring skills, and (6) improve the quality of research.
Relevance to Public Health: The proposed research is expected to reveal the driving principles of a regulatory pathway that exists in multiple bacterial species, including pathogens. Components of the pathway may serve as targets for new antimicrobial compounds.
描述(由申请人提供):细菌细胞已经进化出多种调节机制,以确保其亚细胞结构和细胞器,如毛和鞭毛,被正确地组装和定位。我们的目标是确定一个保守调控系统的核心组成部分,以及它们之间的相互作用,是如何适应不同生物体的特殊需求的。在新月形茎杆菌中,该调节系统的蛋白质成分(PodJ、DivJ和PleC)遵循亚细胞定位的确定模式,导致细胞形态不对称:细胞器特异性地在一端发育,而在另一端不发育。调控成分在Sinorhizobium meliloti中是保守的,Sinorhizobium meliloti是一种在共生过程中诱导植物根系形成根瘤的相关细菌。然而,与月牙草相比,S. meliloti细胞在形态上是对称的。我们将研究这些保守成分如何在S. meliloti中调节表面细胞器的组装以及这种调节如何影响宿主-微生物的相互作用。(1)我们将首先确定调节蛋白的定位模式和缺乏这些蛋白的零突变体的表型。(2)然后,我们将检测和分析突变体转录谱的变化,看看这些蛋白质是否控制着不同物种中相似基因的表达。(3)我们还将评估突变是否会破坏细菌与其宿主之间的相互作用,从而阻止有效结瘤。除了这些研究目标外,在资深科学家的指导下,主要研究者还希望实现以下发展目标:(4)建立独立的研究小组;(5)提高指导技能;(6)提高研究质量。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Trapping and rotating microparticles and bacteria with moiré-based optical propelling beams.
- DOI:10.1364/boe.3.001891
- 发表时间:2012-08-01
- 期刊:
- 影响因子:3.4
- 作者:Zhang P;Hernandez D;Cannan D;Hu Y;Fardad S;Huang S;Chen JC;Christodoulides DN;Chen Z
- 通讯作者:Chen Z
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Joseph Chiung-Chu Chen其他文献
Joseph Chiung-Chu Chen的其他文献
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{{ truncateString('Joseph Chiung-Chu Chen', 18)}}的其他基金
Research Supplement to Promote Diversity in Microbiology
促进微生物学多样性的研究补充
- 批准号:
10362421 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Regulatory network of a conserved polar factor in Sinorhizobium meliloti
苜蓿中华根瘤菌保守极性因子的调控网络
- 批准号:
8514644 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
SC3: Regulation of polar adhesion during Sinorhizobium meliloti infection
SC3:苜蓿中华根瘤菌感染期间极性粘附的调节
- 批准号:
9073175 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Sinorhizobium meliloti factors that affect biofilm formation and symbiosis efficiency
影响生物膜形成和共生效率的苜蓿中华根瘤菌因子
- 批准号:
10190958 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Sinorhizobium meliloti factors that affect biofilm formation and symbiosis efficiency
影响生物膜形成和共生效率的苜蓿中华根瘤菌因子
- 批准号:
10672247 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Research Supplement to Promote Diversity in Microbiology
促进微生物学多样性的研究补充
- 批准号:
10449502 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Sinorhizobium meliloti factors that affect biofilm formation and symbiosis efficiency
影响生物膜形成和共生效率的苜蓿中华根瘤菌因子
- 批准号:
10460456 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Regulatory network of a conserved polar factor in Sinorhizobium meliloti
苜蓿中华根瘤菌保守极性因子的调控网络
- 批准号:
8724515 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Regulatory network of a conserved polar factor in Sinorhizobium meliloti
苜蓿中华根瘤菌保守极性因子的调控网络
- 批准号:
8339069 - 财政年份:2012
- 资助金额:
$ 3.47万 - 项目类别:
Regulated Assembly of Subcellular Structures in alpha-Proteobacteria
α-变形菌亚细胞结构的调控组装
- 批准号:
7342328 - 财政年份:2008
- 资助金额:
$ 3.47万 - 项目类别:
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