B CELL MEMORY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 免疫记忆是脊椎动物免疫系统的一个标志。第一次抗原暴露会导致缓慢而温和的免疫反应，而重复暴露，即使在许多年后，也会导致比初次暴露大2-3个数量级的快速和夸大的反应。在体液免疫的情况下，召回反应效率的提高是由于产生了放大水平的抗原特异性抗体，以及它们产生的动力学加快。目前的理论认为，这是由于长寿命的抗原特异性记忆B细胞的选择性激活所致。 仅限制对记忆细胞的次级反应的一个不利之处是，当病原体继续进化时，记忆B细胞池的谱系保持不变。在这里，我们正在努力确定在二次反应中，抗原特异性B细胞是否与记忆细胞一起参与。我们正在证明，在体内抗原和先前存在的抗体之间形成的免疫复合体来自最初的反应，激活这些原始的B细胞，诱导它们以加速的动力学和增加的幅度做出反应。在每次抗原暴露后不断招募新的B细胞克隆，使免疫系统能够与迅速变化的病原体保持同步。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Immunological memory is a hallmark of the vertebrate immune system. The first antigenic exposure leads to a slow and modest immune response while repeat exposure, even many years later, leads to a rapid and exaggerated response that is 2-3 orders of magnitude greater than the primary. In the case of humoral immunity, the increased efficacy of recall responses is due to the production of amplified levels of antigen-specific antibody, as well as the accelerated kinetics of their production. Current dogma suggests that this is due to selective activation of long-lived antigen-specific memory B cells. A downside of restricting secondary responses solely to memory cells is that the repertoire of the memory B cell pool remains static while pathogens continue to evolve. Here we are working to determine whether during secondary responses na¿ve, antigen-specific B cells participate alongside memory cells. We are showing that immune complexes formed in vivo between the antigen and pre-existing antibodies from the primary response, activate these naive B cells, inducing them to respond with accelerated kinetics and increased magnitude. The continued recruitment of new B cell clones following each antigenic exposure enables the immune system to stay abreast of rapidly changing pathogens.