ANATOMIC PATHOLOGY CORE

解剖病理学核心

• 批准号: 8173004
• 负责人: PETER J DIDIER
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173004
• 关键词: Acquired Immunodeficiency Syndrome; Anatomy; Animals; Antibodies; Area; Autopsy; Bathing; Biopsy; Cause of Death; Computer Retrieval of Information on Scientific Projects Database; Computer Workstations; Confocal Microscopy; Disease; Funding; Grant; Histology; Infectious Agent; Institution; Label; Laboratories; Microscope; Microtome - medical device; Modeling; Molecular Probes; Monitor; Pathology; Policies; Preparation; Process; Research; Research Personnel; Resources; Services; Slide; Source; Staining method; Stains; Thick; Time; Tissue Banks; Tissues; United States National Institutes of Health; Water; experience; instrumentation; molecular pathology; programs; sample fixation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Anatomic Pathology Core consists of the Necropsy, Histology, and Tissue Collection and Distribution Units and is responsible for post-mortem examinations, tissue collection and distribution, fixation, processing, slide preparation, routine and special staining. It is a center policy that a cause of death is determined, if possible, for all animals that die at the center, whether of natural or experimental causes. This allows us to monitor and document all infectious agents and disease processes that occur within the colony and provides an opportunity to discover new diseases, which may be useful models. In addition, most major research areas at the center depend heavily on anatomic pathology support as an integral part of the research. In 2009, a total of 814 necropsies and 356 biopsies were performed by the Core. The Anatomic Pathology Core Laboratory instrumentation includes 3 Microm HM325 microtomes and one automated Leica RM2145 microtome for preparing thin and thick tissue sections for routine H&E and special staining; sections for confocal microscopy, labeling studies with antibodies and molecular probes (performed in the Molecular Pathology Core). We also utilize a Leica ASP 300 tissue processor, a Tissue-Tek embedding station, and assorted water baths, ovens, microscopes and a computer work station. This year the Core produced 22,205 research slides and an additional 3,040 un-sectioned blocks . Currently we employ three full time histologists with a combined 40 years' laboratory experience. The AIDS research program is a major user of core services.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 解剖病理学核心由尸检、组织学和组织收集和分配单位组成，负责尸检、组织收集和分配、固定、处理、玻片制备、常规和特殊染色。 中心的政策是，如果可能的话，确定中心所有死亡动物的死因，无论是自然原因还是实验原因。 这使我们能够监测和记录群体内发生的所有传染源和疾病过程，并提供发现新疾病的机会，这可能是有用的模型。 此外，该中心的大多数主要研究领域在很大程度上依赖于解剖病理学支持作为研究的一个组成部分。 2009 年，核心共进行了 814 例尸检和 356 例活组织检查。解剖病理学核心实验室仪器包括 3 微米 HM325 切片机和一台自动化 Leica RM2145 切片机，用于制备用于常规 H&E 和特殊染色的薄和厚组织切片；共聚焦显微镜切片、抗体和分子探针标记研究（在分子病理学核心中进行）。 我们还使用 Leica ASP 300 组织处理机、Tissue-Tek 包埋站以及各种水浴、烤箱、显微镜和计算机工作站。 今年，Core 制作了 22,205 个研究幻灯片和另外 3,040 个未切片的块。 目前，我们聘用了三名全职组织学家，总共拥有 40 年的实验室经验。 艾滋病研究计划是核心服务的主要用户。