ANATOMIC PATHOLOGY CORE

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 解剖病理学核心由尸检、组织学和组织采集和分配单元组成，负责尸检、组织采集和分配、固定、处理、载玻片制备、常规和特殊染色。中心的政策是，如果可能的话，确定所有在中心死亡的动物的死亡原因，无论是自然原因还是实验原因。这使我们能够监测和记录殖民地内发生的所有传染因子和疾病过程，并提供了发现新疾病的机会，这可能是有用的模型。此外，该中心的大多数主要研究领域在很大程度上依赖于解剖病理学支持作为研究的一个组成部分。2010年，核心小组共进行了822次尸检和450次活组织检查。这比2009年有所增加。 解剖病理学核心实验室仪器包括3台Microm HM 325切片机和一台自动Leica RM 2145切片机，用于制备常规H&E和特殊染色的薄和厚组织切片;共聚焦显微镜切片，抗体和分子探针标记研究（在分子病理学核心中进行）。我们还使用Leica ASP 300组织处理器、Tissue-Tek包埋站、以及各种水浴、烤箱、显微镜和计算机工作站。 2010年，实验室增加了Leica autostainer XL自动载玻片染色机和盖玻片。 今年的核心制作了26，453张研究幻灯片和另外1，887个未切片的块。目前，我们雇用了三名全职和一名兼职组织学家，拥有40年的实验室经验。艾滋病研究方案是核心服务的主要用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Anatomic Pathology Core consists of the Necropsy, Histology, and Tissue Collection and Distribution Units and is responsible for post-mortem examinations, tissue collection and distribution, fixation, processing, slide preparation, routine and special staining. It is a center policy that a cause of death is determined, if possible, for all animals that die at the center, whether of natural or experimental causes. This allows us to monitor and document all infectious agents and disease processes that occur within the colony and provides an opportunity to discover new diseases, which may be useful models. In addition, most major research areas at the center depend heavily on anatomic pathology support as an integral part of the research. In 2010, a total of 822 necropsies and 450 biopsies were performed by the Core. This represents an increase over 2009. The Anatomic Pathology Core Laboratory instrumentation includes 3 Microm HM325 microtomes and one automated Leica RM2145 microtome for preparing thin and thick tissue sections for routine H&E and special staining; sections for confocal microscopy, labeling studies with antibodies and molecular probes (performed in the Molecular Pathology Core). We also utilize a Leica ASP 300 tissue processor, a Tissue-Tek embedding station, and assorted water baths, ovens, microscopes and a computer work station. In 2010, a Leica autostainer XL automated slide stainer and cover slipper was added to the lab. This year the Core produced 26,453 research slides and an additional 1,887 un-sectioned blocks . Currently we employ three full time, and one part-time histologist, with a combined 40 years' laboratory experience. The AIDS research program is a major user of core services.