Genetic and genomic tools for the emerging model organsism, Nasonia

用于新兴模式生物 Nasonia 的遗传和基因组工具

基本信息

  • 批准号:
    8079968
  • 负责人:
  • 金额:
    $ 4.09万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-07-01 至 2011-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Nasonia is a genus of four insect species that is quickly emerging as a model system, particularly for the genetics of complex traits, development, and microbial-host interactions. It has several features that make it an excellent genetic system. These include ease of handling, short generation time, male haploidy, interfertile species, visible and molecular markers, and a wealth of phenotypes of biological and medical relevance. The form of sex determination, called haplodiploidy, makes Nasonia particularly suited for genetic studies. Females are diploid and develop from fertilized eggs, whereas males are haploid and develop parthenogenetically from unfertilized eggs. This allows geneticists to exploit many of the advantages of haploid genetics in an otherwise complex eukaryotic organism. Furthermore, Nasonia readily inbreeds, permitting production of isogenic lines, and the four species in the genus are inter-fertile (after removal of the endosymbiont Wolbachia), facilitating movement of genes between the species for efficient positional cloning of quantitative trait loci (QTL). Full (6X) genome sequencing of N. vitripennis (Nv) and partial (1X) sequencing of the interfertile species N. giraulti (Ng) and N. longicornis (Nl) is now completed. This genome project provides a wealth of interspecies polymorphisms (SNPs, indels, microsatelites) to facilitate positional cloning. A gene affecting tissue-specific cell growth has already been cloned, and other efforts are underway to dissect the genetic basis of wing-development, embryonic development, parthenogenesis, bacterial-host interactions, host preference, sex determination, diapause, male pheromones, and courtship and reproduction. In addition, systemic RNAi and transformation have recently been demonstrated in Nasonia. We propose to further develop a set of genomic and genetic tools that will allow the research community to exploit more efficiently the particular advantages of this emerging genetic system. Our specific goals are to (a) complete mapping of the Nasonia scaffolds using an efficient array-based approach and interspecies crosses, (b) analyze and develop tools for exploiting the Nasonia transcriptome, (c) Improve RNAi and transgenesis methods and tools, and (d) develop a set of genetic tools to enhance efficient positional cloning. These resources will both improve discovery and cloning of QTL in Nasonia, and its utility to other research systems for comparative genetic and genomic research. The proposed tool development will greatly enhance the Nasonia system as a genetic and comparative model, and allow fuller utilization of the Nasonia genome. It has the strong support of the Nasonia and other relevant research communities.
描述(由申请人提供):Nasonia 是四种昆虫的一个属,它正在迅速成为模型系统,特别是在复杂性状的遗传学、发育和微生物与宿主相互作用方面。它具有几个使其成为优秀遗传系统的特征。这些包括易于处理、短世代时间、雄性单倍体、不育物种、可见和分子标记,以及丰富的生物和医学相关表型。性别决定的形式称为单倍二倍体,使 Nasonia 特别适合遗传研究。雌性是二倍体,由受精卵发育而来,而雄性是单倍体,由未受精卵孤雌发育而来。这使得遗传学家能够在复杂的真核生物中利用单倍体遗传学的许多优点。此外,Nasonia 很容易近亲繁殖,从而产生等基因系,并且该属的四个物种是互育的(去除内共生体沃尔巴克氏体后),促进基因在物种之间的移动,从而实现数量性状基因座(QTL)的有效位置克隆。 N. vitripennis (Nv) 的完整 (6X) 基因组测序以及不育物种 N. giraulti (Ng) 和 N. longicornis (Nl) 的部分 (1X) 测序现已完成。该基因组计划提供了大量的种间多态性(SNP、插入缺失、微卫星)以促进定位克隆。影响组织特异性细胞生长的基因已经被克隆,并且其他工作正在进行中,以剖析翅膀发育、胚胎发育、孤雌生殖、细菌-宿主相互作用、宿主偏好、性别决定、滞育、雄性信息素以及求偶和繁殖的遗传基础。此外,最近在 Nasonia 中也证实了系统性 RNA 干扰和转化。我们建议进一步开发一套基因组和遗传工具,使研究界能够更有效地利用这一新兴遗传系统的特殊优势。我们的具体目标是(a)使用高效的基于阵列的方法和种间杂交完成 Nasonia 支架的绘制,(b)分析和开发用于利用 Nasonia 转录组的工具,(c)改进 RNAi 和转基因方法和工具,以及(d)开发一套遗传工具以增强有效的定位克隆。这些资源将改善 Nasonia QTL 的发现和克隆,及其在其他研究系统中进行比较遗传和基因组研究的实用性。拟议的工具开发将极大地增强 Nasonia 系统作为遗传和比较模型的能力,并允许更充分地利用 Nasonia 基因组。它得到了 Nasonia 和其他相关研究团体的大力支持。

项目成果

期刊论文数量(0)
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JOHN Haynes WERREN其他文献

JOHN Haynes WERREN的其他文献

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{{ truncateString('JOHN Haynes WERREN', 18)}}的其他基金

Exploring the Venom Repertoire of Parasitoids
探索寄生蜂的毒液
  • 批准号:
    8518396
  • 财政年份:
    2011
  • 资助金额:
    $ 4.09万
  • 项目类别:
Exploring the Venom Repertoire of Parasitoids
探索寄生蜂的毒液
  • 批准号:
    8896279
  • 财政年份:
    2011
  • 资助金额:
    $ 4.09万
  • 项目类别:
Exploring the Venom Repertoire of Parasitoids
探索寄生蜂的毒液
  • 批准号:
    8307280
  • 财政年份:
    2011
  • 资助金额:
    $ 4.09万
  • 项目类别:
Exploring the Venom Repertoire of Parasitoids
探索寄生蜂的毒液
  • 批准号:
    8712511
  • 财政年份:
    2011
  • 资助金额:
    $ 4.09万
  • 项目类别:
Exploring the Venom Repertoire of Parasitoids
探索寄生蜂的毒液
  • 批准号:
    8179467
  • 财政年份:
    2011
  • 资助金额:
    $ 4.09万
  • 项目类别:
Genetic and genomic tools for the emerging model organsism, Nasonia
用于新兴模式生物 Nasonia 的遗传和基因组工具
  • 批准号:
    7844925
  • 财政年份:
    2008
  • 资助金额:
    $ 4.09万
  • 项目类别:
Genetic and genomic tools for the emerging model organsism, Nasonia
用于新兴模式生物 Nasonia 的遗传和基因组工具
  • 批准号:
    8068907
  • 财政年份:
    2008
  • 资助金额:
    $ 4.09万
  • 项目类别:
Genetic and genomic tools for the emerging model organsism, Nasonia
用于新兴模式生物 Nasonia 的遗传和基因组工具
  • 批准号:
    7499865
  • 财政年份:
    2008
  • 资助金额:
    $ 4.09万
  • 项目类别:
Genetics of Wing and Cell-Size Evolution in Nasonia
Nasonia 翅膀的遗传学和细胞大小的进化
  • 批准号:
    7198114
  • 财政年份:
    2005
  • 资助金额:
    $ 4.09万
  • 项目类别:
Genetics of Wing and Cell-Size Evolution in Nasonia
Nasonia 翅膀的遗传学和细胞大小的进化
  • 批准号:
    7674467
  • 财政年份:
    2005
  • 资助金额:
    $ 4.09万
  • 项目类别:

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