Contrasting environmental and genetic controls of alternative phenotypes

对比环境和遗传对替代表型的控制

• 批准号: 8072579
• 负责人: JENNIFER A BRISSON
• 金额: $ 1.18万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-24 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8072579
• 关键词: Address; Adult; Aphids; Apis; Biological Assay; Biological Models; Biology; Complex; Crowding; Cues; Development; Disease susceptibility; Environment; Exhibits; Female; Gas Chromatography; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Polymorphism; Genetic Recombination; Genetic Variation; Genomics; Genotype; Goals; Height; Individual; Link; Maps; Mediating; Metabolic; Molecular Genetics; Morphology; Names; Nature; Pathway interactions; Phenotype; Pisum sativum; Population; Predisposition; Production; RNA Sequences; Research; Science; Signal Transduction; Stimulus; Study models; Systems Biology; Variant; Weight; Wing; asexual; base; developmental genetics; insight; male; metabolomics; response

The proposed research addresses a fundamental question in biology: how do the environment and genotype interact to produce the phenotype? The pea aphid, an emerging genomic model system, exhibits dramatically different adult phenotypes of winged or unwinged morphs that are induced by environmental conditions in asexual females (a polyphenism) and by a single unidentified genetic locus in males (a polymorphism). Further, genetic variation for the female wing polyphenism segregates with the male wing polymorphism suggesting that the developmental networks underlying the polyphenism and polymorphism are not independent. I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental signals and genetic signals interact to produce fundamentally similar adult alternative morphologies.

这项研究解决了生物学中的一个基本问题：环境和基因型如何相互作用以产生表型？豌豆蚜虫，一个新兴的基因组模型系统，表现出显着不同的成年表型的有翅或无翅的变体，诱导的环境条件下，在无性繁殖的女性（多型）和男性（多态性）的一个单一的身份不明的遗传位点。此外，遗传变异的女性翼多型分离与男性翼多态性表明，潜在的多型和多态性的发展网络是不独立的。我建议（1）定位和鉴定雄性多态性基因，以确定雄性多态性的发育遗传基础，（2）描述无性雌性对翅诱导刺激的转录和代谢组学反应，以确定雌性多型性的发育遗传基础;（3）使用系统生物学方法将（1）和（2）的结果联系起来。提供一个关于环境信号和遗传信号如何相互作用产生基本相似的 成人另类形态