Contrasting environmental and genetic controls of alternative phenotypes

对比环境和遗传对替代表型的控制

• 批准号: 7512002
• 负责人: JENNIFER A BRISSON
• 金额: $ 9万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-29 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7512002
• 关键词: Address; Adult; Aphids; Apis; Area; Award; Biological; Biological Assay; Biological Models; Biology; California; Chromosome Mapping; Complement; Complex; Computational Molecular Biology; Condition; Crowding; Cues; DNA; Development; Disease susceptibility; Environment; Exhibits; Female; Gas Chromatography; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Polymorphism; Genetic Recombination; Genetic Variation; Genome; Genomics; Genotype; Goals; Height; Individual; Institution; Laboratories; Link; Maps; Mediating; Mentors; Metabolic; Molecular Genetics; Morphology; Names; Nature; Pathway interactions; Phase; Phenotype; Pisum sativum; Population; Positioning Attribute; Predisposition; Production; Research; Research Personnel; Science; Signal Transduction; Staging; Stimulus; Study models; System; Systems Biology; Training; Universities; Variant; Weight; Wing; asexual; base; design; developmental genetics; insight; male; metabolomics; programs; response; retinal rods; transcriptomics

DESCRIPTION (provided by applicant): The proposed research addresses a fundamental question in biology: how do the environment and genotype interact to produce the phenotype? The pea aphid, an emerging genomic model system, exhibits dramatically different adult phenotypes of winged or unwinged morphs that are induced by environmental conditions in asexual females (a polyphenism) and by a single unidentified genetic locus in males (a polymorphism). Further, genetic variation for the female wing polyphenism segregates with the male wing polymorphism suggesting that the developmental networks underlying the polyphenism and polymorphism are not independent. I propose to (1) map and identify the male polymorphism gene to determine the developmental genetic basis of the male polymorphism, (2) characterize the transcriptional and metabolomic response to wing-inducing stimuli in the asexual females to determine the developmental genetic basis of the female polyphenism and (3) use a systems biology approach to link the results from (1) and (2) to provide a cohesive picture of how environmental signals and genetic signals interact to produce fundamentally similar adult alternative morphologies. The year-long mentored stage of this award focuses on developing a holistic view of the biological systems involved in a training environment that excels in systems and network biology: the laboratory of Dr. Sergey Nuzhdin in the Molecular and Computational Biology section at the University of Southern California. Following the mentored period, I will transition to a tenure-track position at an academic research institution. Relevance: All phenotypes, from our height and weight to our disease susceptibility, result from a complex interplay between our genes and our environment. This project aims to illuminate basic rules acting to integrate environmental and genetic signals in the production of the phenotype.

描述（由申请人提供）：拟议的研究解决了生物学中的一个基本问题：环境和基因型如何相互作用以产生表型？豌豆蚜虫是一种新兴的基因组模型系统，在无性繁殖的雌性蚜虫中，有翅或无翅蚜虫的成虫表型是由环境条件诱导的（多表型），而在雄性蚜虫中，一个未识别的遗传位点（多态性）则是由环境条件诱导的。此外，雌性翼多酚的遗传变异与雄性翼多酚的遗传变异是分离的，这表明多酚和多态性背后的发育网络并不是独立的。我建议：(1)绘制和鉴定男性多态性基因，以确定男性多态性的发育遗传基础；(2)描述无性雌性对翅膀诱导刺激的转录和代谢组反应，以确定雌性多表型的发育遗传基础；(3)使用系统生物学方法将(1)和(2)的结果联系起来，提供环境信号和遗传信号如何相互作用以产生基本相似的成年替代形态的连贯图景。该奖项为期一年的指导阶段侧重于在系统和网络生物学方面的培训环境中发展生物系统的整体观点：南加州大学分子和计算生物学部门Sergey Nuzhdin博士的实验室。指导期结束后，我将转任学术研究机构的终身职位。相关性：所有的表型，从我们的身高和体重到我们的疾病易感性，都是我们的基因和环境之间复杂的相互作用的结果。该项目旨在阐明在表型产生中整合环境和遗传信号的基本规则。