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Using Arabidopsis to uncover interactions between phytohormone signaling pathways

利用拟南芥揭示植物激素信号通路之间的相互作用

基本信息

批准号：
8013311
负责人：
Lucia Strader
金额：
$ 8.98万
依托单位：
RICE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-15 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8013311
关键词：
Abscisic Acid Affinity Chromatography Agriculture Arabidopsis Area Auxins Binding Biochemical Biochemical Genetics Blood Vessels Cell division Characteristics Complement Development Dissection Embryonic Development Environment Ethylenes Excision Exhibits Fruit Genes Genetic Research Genetic Transcription Germination Goals Growth Growth and Development function Hormones Knowledge Learning Lesion MAPK Signaling Pathway Pathway Mentors Mitogen-Activated Protein Kinases Molecular Molecular Genetics Mouse-ear Cress Mutation Nature Pathway interactions Perception Phenotype Phosphoric Monoester Hydrolases Plant Growth Regulators Plant Leaves Plant Model Plant Roots Plants Postdoctoral Fellow Principal Investigator Protein Biosynthesis Protein phosphatase Proteins Regulation Reporter Repressor Proteins Research Resistance Resources Rice Role Saline Seeds Signal Pathway Signal Transduction Site Soil Specificity Stress Students Testing Time Training Universities base biological adaptation to stress career indoleacetic acid insight mutant novel positional cloning public health relevance research study response senescence stress tolerance tool

项目摘要

DESCRIPTION (provided by applicant): Candidate: The candidate's long-term career goal is to become a principal investigator in an academic setting, studying the interaction networks involved in phytohormone response. During the training period, she will learn biochemical research approaches to complement her graduate applied agricultural and post- doctoral molecular genetic research. Training Environment: Rice University provides an ideal training environment because of its quality research and intimate setting. Dr. Bonnie Bartel, the mentor for this project, is a leader in Arabidopsis research. Further, she is an active mentor who devotes the majority of her time to her research and her postdocs and students. Research: The long-term goal of this project is to enhance understanding of the signaling network that connects the phytohormones auxin, abscisic acid (ABA), and ethylene. The dual-specificity protein phosphatase, IBR5, the subject of this proposal, is a novel point of interaction between auxin, ABA, and ethylene signal transduction networks. Several interconnected approaches will use IBR5 to gain greater understanding of the relationship among these phytohormones. Firstly, IBR5-interacting proteins will be identified (Aim 1); these may include substrates and regulators of the IBR5 phosphatase. Additionally, second-site ibr5 modifiers will be analyzed (Aim 2) to determine whether sensitivity to auxin, ABA, and ethylene can be separated, or if they are interconnected in inseparable ways. Identification of defective genes in these modifiers (Aim 3) will allow molecular elucidation of interactions between these phytohormone pathways. These studies will contribute to understanding of which signaling components are shared among these three pathways and which are not shared, and will identify additional nodes in the phytohormone signaling network. In addition to expanding our basic knowledge, a detailed understanding of these three phytohormones, involved in growth responses, stress tolerance, and fruit ripening, may provide insight for the eventual improvement of plants of agricultural or medicinal importance. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting plants to high stress areas (i.e., areas of low rainfall or saline soils). Public Health Relevance: Auxin, abscisic acid, and ethylene are three plant hormones controlling many aspects of growth and development, such as growth responses, stress tolerance, and fruit ripening. Understanding of the signaling interactions between theses phytohormones, in addition to contributing to the general knowledge of how these hormones act, may provide insight for the eventual improvement of agriculturally- and medicinally-important plants. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting crops to high stress areas (i.e., areas of low rainfall or saline soils).

描述（由申请人提供）：候选人：候选人的长期职业目标是成为学术环境中的首席研究员，研究植物激素反应中涉及的相互作用网络。在培训期间，她将学习生物化学研究方法，以补充她的研究生应用农业和博士后分子遗传学研究。培训环境：莱斯大学提供了一个理想的培训环境，因为它的质量研究和亲密的设置。邦尼·巴特尔博士，这个项目的导师，是拟南芥研究的领导者。此外，她是一个积极的导师谁把她的大部分时间都花在她的研究和她的博士后和学生。研究：该项目的长期目标是加强对连接植物激素生长素，脱落酸（阿坝）和乙烯的信号网络的理解。双特异性蛋白磷酸酶IBR 5是生长素、阿坝和乙烯信号转导网络之间相互作用的一个新点。几种相互关联的方法将使用IBR 5来更好地了解这些植物激素之间的关系。首先，将鉴定IBR 5相互作用蛋白（目的1）;这些蛋白可能包括IBR 5磷酸酶的底物和调节剂。此外，第二个网站ibr 5修改器将进行分析（目的2），以确定是否敏感生长素，阿坝和乙烯可以分开，或者如果它们是相互关联的不可分割的方式。鉴定这些修饰剂中的缺陷基因（目的3）将允许从分子水平阐明这些植物激素途径之间的相互作用。这些研究将有助于了解这三种途径中哪些信号组分是共享的，哪些不是共享的，并将确定植物激素信号网络中的其他节点。除了扩大我们的基本知识，详细了解这三种植物激素，参与生长反应，胁迫耐受性和果实成熟，可能会为最终改善农业或药用植物的重要性提供见解。例如，确定如何在不改变生长特性（生长素感知）或成熟（乙烯感知）的情况下增加胁迫耐受性（阿坝感知）可能是使植物适应高胁迫区域（即，低降雨量或盐渍土地区）。公共卫生相关性：生长素、脱落酸和乙烯是三种植物激素，它们控制着生长和发育的许多方面，如生长反应、胁迫耐受性和果实成熟。了解这些植物激素之间的信号相互作用，除了有助于这些激素如何作用的一般知识，可能会提供洞察力的农业和医学重要的植物的最终改进。例如，确定如何在不改变生长特性（生长素感知）或成熟（乙烯感知）的情况下增加胁迫耐受性（阿坝感知）可能是使作物适应高胁迫区域（即，低降雨量或盐渍土地区）。