Auxin Response Factors as a model of transcriptional control
生长素反应因子作为转录控制模型
基本信息
- 批准号:10640222
- 负责人:
- 金额:$ 39.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:4-ethoxymethylene-2-phenyl-2-oxazoline-5-oneAutomobile DrivingAuxinsBackBehaviorBindingBiochemistryBiophysicsChargeChromatin Remodeling FactorComplexCullin ProteinsDevelopmentDiseaseElectrostaticsEventF Box DomainF-Box ProteinsFaceFamilyGene ActivationGeneticGenetic TranscriptionHormonesKnowledgeLigandsMediatingMissionModelingMolecularNatureOrganismPhase TransitionPhysical condensationPlantsPoint MutationPositioning AttributeProcessProteinsReagentRegulationRepressionRepressor ProteinsResearchSideSignal TransductionStructureSystemTranscription CoactivatorTranscription RepressorTranscriptional RegulationUnited States National Institutes of Healthderepressiongene repressioninsightinterdisciplinary approachmemberplant growth/developmentprotein degradationprotein foldingreceptorrecruitresponsetranscription factortransport inhibitorubiquitin-protein ligase
项目摘要
Project Summary
Hormone-mediated modulation of gene activation or repression through transcription factors is central to all
organisms. AUXIN RESPONSE FACTOR (ARF) transcription factors are critical modulators of plant growth
and provide an ideal model for exploring hormone control of gene activation and repression. Of the 23-member
ARF family, five are considered transcriptional activators and 18 are considered transcriptional repressors,
allowing for study of both of these activities in a single family.
Under low auxin concentrations, Aux/IAA proteins repress ARF transcription factors via direct interaction
and recruitment of chromatin remodeling factors. When auxin concentrations are high, a co-receptor complex,
comprised of an F-box protein from the TRANSPORT INHIBITOR REPONSE1 (TIR1) family and an Aux/IAA
repressor protein, directly binds auxin. The F-box protein participates in a Skp1-Cullin-F-box (SCF) E3 ubiquitin
ligase, which targets the Aux/IAA protein for degradation. This degradation event relieves ARF transcription
factor repression, allowing auxin-regulated transcription. This receptor-ligand interaction allows a very short
signal transduction chain to facilitate rapid transcriptional responses to auxin.
To understand the molecular underpinnings of ARF-ARF and ARF-Aux/IAA interactions, our lab solved the
structure of the domain driving these interactions, finding that it folds into a Type I/II Phox and Bem1 (PB1)
domain. Within this domain, there is a positively charged and a negatively charged electrostatic face on
opposing sides, creating a Janus-like protein fold. This allows for front-to-back ARF oligomerization (similar to
a set of bar magnets) in the packed crystal, in solution, and in the plant.
In addition to the well-studied repression – derepression mechanism of regulation, our lab has discovered
that activity of a subset of ARFs can be regulated by protein phase transition driven by the combination of PB1
oligomerization and an intrinsically disordered region. Phase transition of these ARFs appears to modulate
responsiveness to auxin in a developmentally relevant context. We have further found that many ARFs are
regulated by proteasomal degradation and have identified an E3 ubiquitin ligase involved in this process.
Finally, ARF interactions can be easily manipulated using PB1 domain point mutations, allowing us to direct
ARF interactions for study. Using ARFs as a model will allow us to interrogate transcription factor function in an
easily manipulated system to yield broad insight into many transcription factors.
We are aided in our efforts by our multidisciplinary approach, extensive auxin-related molecular toolkit, and
unique reagents generated by our lab. Our lab's expertise in genetics and biochemistry/biophysics, combined
with our recent discoveries of ARF condensation and proteasomal degradation, makes us well positioned to
drive forward our understanding of phase transition and other mechanisms in regulation of transcription factor
activity.
项目总结
项目成果
期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
ARF19 Condensation in the Arabidopsis Stomatal Lineage.
- DOI:10.17912/micropub.biology.000708
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Kuan, Chi;Strader, Lucia C;Morffy, Nicholas
- 通讯作者:Morffy, Nicholas
Connected phytohormone biosynthesis pathways at the core of growth-stress tradeoffs.
- DOI:10.1016/j.molp.2022.06.005
- 发表时间:2022-07-04
- 期刊:
- 影响因子:27.5
- 作者:Sageman-Furnas, Katelyn;Strader, Lucia
- 通讯作者:Strader, Lucia
Nucleocytoplasmic partitioning as a mechanism to regulate Arabidopsis signaling events.
- DOI:10.1016/j.ceb.2021.01.006
- 发表时间:2021-04
- 期刊:
- 影响因子:7.5
- 作者:Allen JR;Strader LC
- 通讯作者:Strader LC
Defying Gravity: WEEP promotes negative gravitropism in Prunus persica (peach) shoots and roots by establishing asymmetric auxin gradients.
对抗重力:WEEP 通过建立不对称的生长素梯度来促进桃(桃)芽和根的负向地性。
- DOI:10.1101/2023.05.26.542472
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Kohler,AndreaR;Scheil,Andrew;HillJr,JosephL;Allen,JeffreyR;Al-Haddad,JameelM;Goeckeritz,CharityZ;Strader,LuciaC;Telewski,FrankW;Hollender,CourtneyA
- 通讯作者:Hollender,CourtneyA
Emerging Roles for Phase Separation in Plants.
- DOI:10.1016/j.devcel.2020.09.010
- 发表时间:2020-10-12
- 期刊:
- 影响因子:11.8
- 作者:Emenecker RJ;Holehouse AS;Strader LC
- 通讯作者:Strader LC
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Lucia Strader其他文献
Lucia Strader的其他文献
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{{ truncateString('Lucia Strader', 18)}}的其他基金
Auxin Response Factors as a model of transcriptional control
生长素反应因子作为转录控制模型
- 批准号:
10188569 - 财政年份:2020
- 资助金额:
$ 39.13万 - 项目类别:
Auxin Response Factors as a model of transcriptional control
生长素反应因子作为转录控制模型
- 批准号:
10411950 - 财政年份:2020
- 资助金额:
$ 39.13万 - 项目类别:
NIGMS administrative equipment supplement for R35 GM136338-02
R35 GM136338-02 的 NIGMS 管理设备补充
- 批准号:
10578437 - 财政年份:2020
- 资助金额:
$ 39.13万 - 项目类别:
REGULATION OF AUXIN RESPONSE FACTOR ACTIVITY IN ARABIDOPSIS
拟南芥生长素反应因子活性的调控
- 批准号:
8964073 - 财政年份:2015
- 资助金额:
$ 39.13万 - 项目类别:
REGULATION OF AUXIN RESPONSE FACTOR ACTIVITY IN ARABIDOPSIS
拟南芥生长素反应因子活性的调控
- 批准号:
9127277 - 财政年份:2015
- 资助金额:
$ 39.13万 - 项目类别:
Using Arabidopsis to uncover interactions between phytohormone signaling pathways
利用拟南芥揭示植物激素信号通路之间的相互作用
- 批准号:
8306724 - 财政年份:2010
- 资助金额:
$ 39.13万 - 项目类别:
Using Arabidopsis to uncover interactions between phytohormone signaling pathways
利用拟南芥揭示植物激素信号通路之间的相互作用
- 批准号:
7772531 - 财政年份:2010
- 资助金额:
$ 39.13万 - 项目类别:
Using Arabidopsis to uncover interactions between phytohormone signaling pathways
利用拟南芥揭示植物激素信号通路之间的相互作用
- 批准号:
8013311 - 财政年份:2010
- 资助金额:
$ 39.13万 - 项目类别:
Using Arabidopsis to uncover interactions between phytohormone signaling pathways
利用拟南芥揭示植物激素信号通路之间的相互作用
- 批准号:
8529558 - 财政年份:2010
- 资助金额:
$ 39.13万 - 项目类别:
Using Arabidopsis to uncover interactions between phytohormone signaling pathways
利用拟南芥揭示植物激素信号通路之间的相互作用
- 批准号:
8288374 - 财政年份:2010
- 资助金额:
$ 39.13万 - 项目类别:
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