Protein-Glycan Interaction Resource of the CFG

CFG 的蛋白-聚糖相互作用资源

• 批准号: 8180918
• 负责人: RICHARD D CUMMINGS
• 金额: $ 89.68万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-20 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180918
• 关键词: Address; Affinity; Animals; Antibodies; Area; Benchmarking; Binding; Binding Proteins; Bioinformatics; Biomedical Research; Blood typing procedure; Cell Communication; Collaborations; Communities; Data; Databases; Ensure; Funding; Galactose Binding Lectin; Glass; Glues; Glycobiology; Goals; Grant; Housing; Human; Human Resources; Immune Sera; Investigation; Knowledge; Lectin; Ligands; Mediating; Metadata; Mission; National Institute of General Medical Sciences; Natural Immunity; Polysaccharides; Preparation; Principal Investigator; Printing; Production; Protein Binding; Protein-Carbohydrate Interaction; Proteins; Relative (related person); Research; Research Institute; Research Personnel; Resources; Sampling; Scientist; Screening procedure; Slide; Specificity; Structure; United States National Institutes of Health; Universities; Update; Work; base; blood group; carbohydrate receptor; data sharing; expiration; interest; medical schools; microbial; microorganism; programs; protein function; success; web site

DESCRIPTION (provided by applicant): The preparation and availability of glycan microarrays was developed by the NIGMS/NIH through a Glue-Grant-supported Consortium for Functional Glycomics (CFG). This resource has revolutionized the glycomics field through making available a rich assortment of over 500 immobilized glycans for general screening for interactions with proteins that recognize glycan determinants (glycan-binding proteins or GBPs). This is an application for continued support of this key "legacy" resource due to the expiration of funding for the CFG in July 2011, following successful work for 10 years under the Glue Grant mechanism. Here we propose to continue this resource as the "Protein-Glycan Interaction Resource of the CFG", in which two of the key cores of the CFG, Core D and Core H, will be combined to make available glycan microarrays for wide use by biomedical research community. As currently, investigators interested in using this resource will submit their request to the CFG Website that will be maintained through other legacy funding to the Bioinformatics Core B of the CFG. As currently, a Steering Committee of eminent scientists in the field will periodically review and approve/deny resources for investigators based on the scientific importance and resources available. Following approval, investigators will send samples to Emory University School of Medicine where the Protein-Glycan Interaction Resource will be housed in space currently used by Core H of the CFG and coordinated by Dr. Richard D. Cummings (PD/PI). The glycan microarrays used by the Protein-Glycan Interaction Resource will be provided by the Glycan Array Production Resource coordinated by Dr. James Paulson (PD/PI) at The Scripps Research Institute, and will initially be Version 5.0, but can be expanded as glycans may be contributed to the program by investigators in the field. Glycan microarray analyses will be conducted at Emory and completed results made available to the investigators and to the legacy bioinformatics of the CFG for public posting and curation. This program will ensure the highest level of integrity and systematic excellence in the preparation, utilization, and management of glycan microarray data, and make available to the broad research community this valuable resource. PUBLIC HEALTH RELEVANCE: Glycan microarrays are used by hundreds of different investigators that have accessed them through the NIGMS/NIH Glue-Grant-supported Consortium for Functional Glycomics (CFG). This is a request for legacy funding to continue making available the glycan microarrays and associated databases and bioinformatics capabilities for the broad research community. This resource is unique and not duplicated elsewhere.

描述（由申请方提供）：聚糖微阵列的制备和可用性由NIGMS/NIH通过甘精胰岛素资助的功能性糖组学联盟（CFG）开发。该资源通过提供500多种固定化聚糖的丰富分类，用于一般筛选与识别聚糖决定簇的蛋白质（聚糖结合蛋白或GBP）的相互作用，彻底改变了糖组学领域。这是一项继续支持这一关键“遗产”资源的申请，因为在粘合剂赠款机制下成功工作了10年之后，CFG的供资于2011年7月到期。在这里，我们建议继续这一资源作为“CFG的蛋白质-聚糖相互作用资源”，其中CFG的两个关键核心，核心D和核心H，将被组合，以使可供生物医学研究界广泛使用的聚糖微阵列。与目前一样，有兴趣使用该资源的研究者将向CFG网站提交申请，该网站将通过CFG生物信息学核心B的其他遗留资金进行维护。目前，由该领域著名科学家组成的指导委员会将根据科学重要性和现有资源定期审查和批准/拒绝为调查人员提供资源。批准后，研究人员将把样本送到埃默里大学医学院，在那里，蛋白质-聚糖相互作用资源将被安置在目前由CFG核心H使用的空间内，并由Richard D. Cummings（PD/PI）.蛋白质-聚糖相互作用资源使用的聚糖微阵列将由Scripps研究所的James Paulson博士（PD/PI）协调的聚糖阵列生产资源提供，最初将是5.0版，但可以扩展，因为聚糖可能由该领域的研究者贡献给该计划。聚糖微阵列分析将在埃默里大学进行，完成的结果将提供给研究者和CFG的传统生物信息学部门，以供公开发布和管理。该计划将确保聚糖微阵列数据的制备，利用和管理的最高水平的完整性和系统卓越性，并向广泛的研究社区提供这一宝贵的资源。 公共卫生相关性：聚糖微阵列被数百名不同的研究人员使用，这些研究人员通过NIGMS/NIH Glucose Grant支持的功能性糖组学联盟（CFG）访问它们。这是对遗产资金的请求，以继续为广大研究界提供聚糖微阵列和相关数据库以及生物信息学能力。这个资源是独一无二的，不会在其他地方重复。