CORE C: HISTOPATHOLOGY CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Core C为干细胞Cobre和其他MMCRI研究人员提供专业的组织学服务。服务包括组织固定、包埋、冰冻、石蜡或塑料包埋标本切片，以及某些染色程序。组织学染色包括苏木精-伊红、三色、vonKossa、地衣、vanGieson(和其他需要的染色)。还提供了多种抗体的免疫组织化学染色。核心由一位有30年经验的全职历史学家(凯瑟琳·卡里尔饰)操作，他执行所有的日常操作。这些包括：组织处理(徕卡TP1020)和特殊染色；免疫组织化学；选择方案开发；以及请求处理、订购和核心维护。CORE主管(Volkhard Lindner)负责监督CORE的运作，并积极协助解释和评估组织标本。现场仪器包括：冷冻切片HM505E微型机；石蜡和塑料切片微型机HM355S和HM325微型机；用于石蜡渗透的Fisher组织中心包埋站、Lab-Line 3618型和VWR1410型真空炉；以及幻灯片加热器和水浴。为了进行集中的组织学操作，通过科布尔奖基金将一台自动组织处理机(徕卡TP1020)投入使用。2005年，一台自动玻片着色机(徕卡AUTOSTINER XL)和盖子滑移站(Hyperlean)上线。购买了一台研磨机(2008/2009年)，用于塑料埋入样品的表面处理和塑料埋入样品的组织加工和切片。这一核心是在我们的干细胞科布雷中心很早就建立起来的，现在已经非常高效(和创收)。就像B、D、E核心的情况一样，这个组织病理学核心对于推进我们正在进行的和新提出的项目以及整个计划至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core C provides expert histology services to Stem Cell COBRE, and additional MMCRI investigators. Services include tissue fixation, embedding, sectioning of frozen, paraffin or plastic embedded specimens, and certain staining procedures. Histological stains include Hematoxylin-Eosin, Trichrome, vonKossa, Orcein, vanGieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also provided. The Core is operated by a full-time histologist with 30 years of experience (Kathleen Carrier) who performs all day-to-day operations. These include: tissue processing (Leica TP1020) and special stains; immunohistochemistry; select protocol development; and request processing, ordering and core maintenance. The Core director (Volkhard Lindner) oversees Core operations and actively assists in the interpretation and evaluation of histology specimens. In-place instrumentation includes: Microm HM505E Cryostat for frozen sections; Microm HM355S and Microm HM325 microtome for paraffin and plastic sections; Fisher HistoCenter embedding station, Lab-Line Model 3618 and VWR model 1410 vacuum ovens for paraffin infiltration; and slide warmers and water baths. For centralized histology operation, an automated tissue processor (Leica TP1020) was brought on line through COBRE award funds. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. A grinder was purchased (2008/2009) for surfacing plastic embedded samples and tissue processing and sectioning of plastic embedded specimens. This core was established early-on in our Stem Cell COBRE Center, and is now highly productive (and income-generating). As is the case for cores B, D, E, this Histopathology Core is critical for advancing our ongoing and newly proposed projects, and overall Program.