CORE C: HISTOPATHOLOGY CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 核心C为干细胞COBRE和其他MMCRI研究人员提供专家组织学服务。 服务包括冷冻、石蜡或塑料包埋标本的组织固定、包埋、切片以及某些染色程序。组织学染色剂包括苏木精-伊红、三色、vonKossa、地衣红、vanGieson（以及其他需要的染色剂）。还提供了用多种抗体的免疫组织化学染色。核心由一名拥有30年经验的全职组织学家（Kathleen Carrier）操作，他执行所有日常操作。 其中包括：组织处理（Leica TP 1020）和特殊染色;免疫组织化学;选择方案开发;以及请求处理、订购和核心维护。 核心主管（Rehhard Lindner）负责监督核心操作，并积极协助解释和评价组织学标本。 现场仪表包括：用于冷冻切片的Microm HM 505 E冷冻恒温器;用于石蜡和塑料切片的Microm HM 355 S和Microm HM 325切片机;用于石蜡渗透的Fisher HistoCenter包埋站、Lab-Line 3618型和VWR 1410型真空烘箱;以及载玻片加热器和水浴。对于集中的组织学操作，通过COBRE奖励基金，将自动组织处理器（Leica TP 1020）投入使用。 2005年，一台自动载玻片染色机（Leica Autostainer XL）和盖片站（Hyperclean）上线。 购买了一台研磨机（2008/2009年），用于塑料包埋样本的表面处理以及塑料包埋样本的组织处理和切片。 这个核心是在我们的干细胞COBRE中心早期建立的，现在是高产的（和创收）。 与核心B、D、E的情况一样，该历史学核心对于推进我们正在进行的和新提出的项目以及整个计划至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core C provides expert histology services to Stem Cell COBRE, and additional MMCRI investigators. Services include tissue fixation, embedding, sectioning of frozen, paraffin or plastic embedded specimens, and certain staining procedures. Histological stains include Hematoxylin-Eosin, Trichrome, vonKossa, Orcein, vanGieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also provided. The Core is operated by a full-time histologist with 30 years of experience (Kathleen Carrier) who performs all day-to-day operations. These include: tissue processing (Leica TP1020) and special stains; immunohistochemistry; select protocol development; and request processing, ordering and core maintenance. The Core director (Volkhard Lindner) oversees Core operations and actively assists in the interpretation and evaluation of histology specimens. In-place instrumentation includes: Microm HM505E Cryostat for frozen sections; Microm HM355S and Microm HM325 microtome for paraffin and plastic sections; Fisher HistoCenter embedding station, Lab-Line Model 3618 and VWR model 1410 vacuum ovens for paraffin infiltration; and slide warmers and water baths. For centralized histology operation, an automated tissue processor (Leica TP1020) was brought on line through COBRE award funds. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. A grinder was purchased (2008/2009) for surfacing plastic embedded samples and tissue processing and sectioning of plastic embedded specimens. This core was established early-on in our Stem Cell COBRE Center, and is now highly productive (and income-generating). As is the case for cores B, D, E, this Histopathology Core is critical for advancing our ongoing and newly proposed projects, and overall Program.