CORE C: HISTOPATHOLOGY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该核心为COBRE和其他MMCRI研究人员提供组织学服务。该核心执行组织固定，包埋和切片冷冻，石蜡或塑料包埋标本。Core进行组织染色，包括苏木精-伊红、三色、von Kossa、Orcein、van Gieson（以及其他必要的染色）。多种抗体的免疫组织化学染色也是Core服务的一部分。核心是由一个全职的组织学家在该领域超过25年的经验（凯瑟琳开利）操作。核心主任（Volkhard Lindner）监督核心业务，并协助解释和评估组织学标本。核心使用以下设备：Microm HM505E冷冻切片，Microm HM355S和Microm HM325切片机用于石蜡和塑料切片，Fisher hisocenter包埋站，Lab-Line真空烘箱型号3618和VWR真空烘箱型号1410用于石蜡浸润，载玻片加热器和水浴。为了集中组织操作，一台自动组织处理器（徕卡TP1020）于2004年通过该COBRE奖提供的资金投入使用。一台自动滑动染色机（Leica Autostainer XL）和盖滑动站（Hyperclean）于2005年上线。Kathleen Carrier负责所有日常操作，包括组织处理（Leica TP1020）和特殊污渍；免疫组织化学;请求表单;订货和维修记录；选择协议开发；还有共聚焦显微镜。此外，在线还有HM505E低温恒温器，HM355S和HM325显微切片机，HistoCenter包埋站和石蜡浸润炉。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core provides histology services to COBRE and other MMCRI investigators. The Core performs tissue fixation, embedding, and sectioning of frozen, paraffin or plastic embedded specimens. The Core performs histological stains including Hematoxylin-Eosin, Trichrome, von Kossa, Orcein, van Gieson (and others as needed). Immunohistochemical staining with a variety of antibodies is also part of Core services. The Core is operated by a full time histologist with more than 25 years of experience in the field (Kathleen Carrier). The Core director (Volkhard Lindner) oversees Core operations and assists in the interpretation and evaluation of histology specimens. The Core uses the following equipment: Microm HM505E Cryostat for frozen sections, A Microm HM355S and Microm HM325 microtome for paraffin and plastic sections, A Fisher HistoCenter embedding station, Lab-Line Vacuum oven Model 3618 and VWR vacuum oven model 1410 for paraffin infiltration, Slide Warmers and water baths. For to the centralized histology operation an automated tissue processor (Leica TP1020) was brought on line in 2004 through funds provided by this COBRE award. An automated slide stainer (Leica Autostainer XL) and cover-slipping station (Hyperclean) were brought online in 2005. Kathleen Carrier performs all day-to-day operations including tissue processing (Leica TP1020) & special stains; immunohistochemistry; request forms; ordering and maintenance records; select protocol development; and confocal microscopy. Also, on-line are a HM505E Cryostat, HM355S and HM325 microtomes, HistoCenter embedding station, and ovens for paraffin infiltration.