Histopathology and Microscopy Core

组织病理学和显微镜核心(V·林德纳，核心主任) 目前的组织病理学和显微镜核心于2004年在Cobre in Stem and 祖细胞生物学(8P20GM103465)，Volkhard Lindner博士为核心主任。包括的服务 冷冻和石蜡包埋切片和光谱标本的固定、包埋和加工 常规和特殊的组织学染色和基本的免疫组织化学。我们的员工有需要开发的专业知识 并优化免疫组织化学程序，包括抗原修复，档案组织中的优化， 以及各种检测方法。这个核心的成功吸引了我们的姊妹公司Cobre在血管中的用户 生物学，缅因州医疗中心的其他研究人员，以及地区客户，包括来自邻近IDEA的客户 各州。我们的服务需求很高，并在2011年获得了我们的血管生物学Cobre(P30GM103392) 共同资助组织病理学核心，以增加研究人员以扩大能力，并增加组织 微阵列服务。这一联合筹资机制非常有效，可以有效地扩大规模。 服务的价值。核心及其现有工作人员(核心主任和两名全职工作人员)的业绩超过 在过去的14年里，每年有30,000个程序直接支持近200种出版物。在.期间 间充质和新陈代谢网络的神经调节、塑料包埋和 为了满足科布雷项目的要求，建立了切片和组织形态计量学。为了提高 效率、矿化组织学服务现在已与由 科布雷资助了附近新英格兰大学的组织学和成像核心研究。效率和 在第一阶段，通过购买使用溶剂的更大的组织处理机，能力也得到了提高 效率大大提高，从而减少了成本和危险废物。作为这个科布雷第二阶段的一部分 应用方面，我们建议在Dr。 普鲁多夫斯基以满足第二阶段资助的项目和其他联邦资助研究的需求。目标1 核心是继续提供卓越的服务和培训，提供全套的组织病理学服务。目标2 是将显微镜和共焦显微镜服务和培训无缝集成到我们的工作流程中，以 更好地满足我们Cobre调查人员和这一共享资源的其他用户的需求。科布雷 调查人员将免费获得这些服务，其他调查人员享受极具竞争力的定价。 与过去一样，Core还将继续提供与以下相关的广泛且不断增长的服务 充分利用从以前的科布雷获得的现有设备和仪器的组织学 NIGMS鼓励的支持。

Histopathology and Microscopy Core (V. Lindner, Core Director) The current Histopathology and Microscopy Core was established in 2004 within the COBRE in Stem and Progenitor Cell Biology (8P20GM103465), with Dr. Volkhard Lindner as the Core Director. Services included fixation, embedding, and processing of specimens for frozen and paraffin embedded sections and a spectrum of routine and specialty histological stains and basic immunohistochemistry. Our staff has expertise to develop and optimize immunohistochemistry procedures, including antigen retrieval, optimization in archival tissues, and various detection methods. The success of this Core attracted users from our sister COBRE in Vascular Biology, other investigators at Maine Medical Center, and regional clients, including from neighboring IDeA states. Our services were in high demand, and led in 2011 to our COBRE in Vascular Biology (P30GM103392) co-funding the Histopathology Core to increase research staff for expanded capacity, and add tissue microarray services. This co-funding mechanism has been extremely effective, allowing for efficient expansion of services. The Core with its current staff (Core Director and two full-time staff members) performs more than 30,000 procedures annually and in the past 14 years has directly supported close to 200 publications. During Phase I of this COBRE in Mesenchymal and Neural Regulation of Metabolic Networks, plastic embedding and sectioning as well as histomorphometry were established to meet demands by COBRE projects. To improve efficiency, mineralized tissue histology services have now been consolidated with services provided by the COBRE supported Histology and Imaging Core at the nearby University of New England. Efficiency and capacities were also increased during Phase I by acquisition of a larger tissue processor that uses solvents much more efficiently, thereby reducing costs and hazardous waste. As part of Phase II of this COBRE application, we propose to integrate microscopy and confocal microscopy services under the directorship of Dr. Prudovsky to meet the needs of Phase II supported projects and other federally funded research. Aim 1 of this core is to continue to provide outstanding services and training in a full suite of histopathology services. Aim 2 is to seamlessly integrate microscopy and confocal microscopy services and training into our workflow, to better serve the needs of our COBRE investigators and other users of this shared resource. COBRE investigators will receive these services at no charge, and other investigators enjoy highly competitive pricing. As in the past, the Core will also continue to offer its extensive and growing spectrum of services related to histology with full utilization of existing equipment and instrumentation garnered from previous COBRE support, as encouraged by NIGMS.