SCREENING FOR NOTCH AND SNAIL MUTANTS, AND ROLES OF IGFBP-2

NOTCH 和 SNAIL 突变体的筛选以及 IGFBP-2 的作用

• 批准号: 8360273
• 负责人: THOMAS HOOKER GRIDLEY
• 金额: $ 14.53万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360273
• 关键词: Affect; Centers of Research Excellence; Equilibrium; Family; Fatty acid glycerol esters; Funding; Grant; IGFBP2 gene; Knock-out; Metabolic; Monitor; Morphogenesis; National Center for Research Resources; Pilot Projects; Principal Investigator; Regenerative Medicine; Research; Research Infrastructure; Resources; Role; Screening procedure; Snails; Somatomedins; Source; Stem cells; Transcription Repressor/Corepressor; United States National Institutes of Health; bone; cost; human IGFBP2 protein; insight; lipid biosynthesis; mutant; notch protein; stem cell biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This proposed pilot project seeks to apply metabolic approaches to uncover new roles (and action mechanisms) for factors that may balance progenitor cell apportioning towards bone vs fat formation. One factor is a transcriptional repressor within the Snail family. A second is a co-factor for insulin-like growth factor (IGF) as IGF-binding protein-2 (IGF-BP2). As preliminary results, the conditional knockout of each (as accomplished by Drs Gridley and Rosen) appears to markedly affect adipogenesis and bone morphogenesis. Both global, and mechanistic insight into IGF-BP2 and Snail actions stand to be advanced through metabolic monitoring.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这个拟议的试点项目旨在应用代谢方法来揭示可能平衡祖细胞向骨与脂肪形成分配的因素的新作用（和作用机制）。 一个因子是Snail家族中的转录抑制因子。 第二种是胰岛素样生长因子（IGF）的辅因子，如IGF结合蛋白-2（IGF-BP 2）。 作为初步结果，每种基因的条件性敲除（由Gridley和罗森博士完成）似乎显著影响脂肪形成和骨形态发生。通过代谢监测，对IGF-BP 2和Snail作用的全局和机制的洞察力都将得到推进。