CELL BIOLOGY/MICROINJECTION

细胞生物学/显微注射

• 批准号: 7535433
• 负责人: THOMAS HOOKER GRIDLEY
• 金额: $ 19.4万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7535433
• 关键词: Area; Cancer Center; Cancer Center Support Grant; Cellular biology; Collaborations; Competence; Cryopreservation; Custom; Derivation procedure; ES Cell Line; Electroporation; Ensure; Faculty; Fee-for-Service Plans; Funding; Gene Targeting; Health Services Accessibility; Human Resources; Hybrids; Inbred Strain; Inbred Strains Mice; Laboratories; Laboratory Research; Microinjections; Mission; Molecular Biology; Mus; Names; Operative Surgical Procedures; Phenotype; Process; Productivity; Reproductive Technology; Research; Research Personnel; Resources; Science; Scientist; Services; Technical Expertise; Technology; Training; Transgenic Organisms; Validation; Work; cost; embryonic stem cell; gene function; genetic resource; in vivo; instrumentation; member; reagent testing; reproductive

The mission of the Cell Biology & Microinjection component of Reproductive Sciences is to provide Cancer Center members with access to centralized facilities, instrumentation and technical expertise necessary to allow investigators to carry out embryonic stem (ES) cell line derivation, electroporation, and to create genetically modified mice for in vivo studies of gene function. The Service generates genetically modified mice on a variety of inbred strain and hybrid backgrounds and has derived germline-competent ES cell lines from inbred mouse strains. Germline-competence validation, training, custom projects, and tested reagents and supplies are also offered. The Service, which has previously been supported by Cancer Center support grant (CCSG) funds, works closely with Cryopreservation, Importation and Rederivation within Reproductive Sciences, as well as Phenotyping Sciences to provide Cancer Center members well integrated comprehensive services. In 2004 the Cell Biology & Microinjection Service merged with the Reproductive Technologies Resource to form Reproductive Sciences. The synergy created by the merger allowed management and staff to identify complementary and overlapping functions within the groups; establish new collaborative service offerings; merge similar processes to eliminate redundancy; and combine laboratory, vivarium and office space to maximize productivity. Cell Biology & Microinjection Service personnel work closely with other members of Reproductive Sciences and the Molecular Biology Service to offer Cancer Center investigators seamless access to services. This close internal collaboration insures that investigators with expertise outside of these areas can fully employ current transgenic and gene targeting technology in their own work by utilizing these interactive services. Senior Staff Scientist Dr. Thomas Gridley was named Faculty Advisor (Project Leader) of Cell Biology & Microinjection Service in 1999. He oversees this fee-for-service operation which employs two ES cell technologists, two microinjection technologists, a colony manager and senior operational manager. The Service occupies 1,899 ft2 of laboratory, vivarium and office space in Research Laboratory Building 4 and the Genetics Resources Building. Dr. Gridley communicates with the Cancer Center users, Service staff and Center Administration to ensure that research needs are met in the most efficient, cost-effective and technically current manner.

生殖科学的细胞生物学和微量注射部分的使命是提供癌症 中心成员有权使用集中设施、仪器和必要的技术专长 允许研究人员进行胚胎干细胞系的衍生、电穿孔和创造 用于体内基因功能研究的转基因小鼠。该服务生产转基因产品 小鼠在各种近交系和杂交背景下，并已获得具有生殖系能力的ES细胞 来自近交系小鼠品系的品系。生殖系-能力验证、培训、定制项目和测试 还提供试剂和用品。这项服务之前一直得到癌症的支持 中心支持赠款(CCSG)基金，与冷冻保存、进口和再衍生密切合作 生殖科学以及表型科学为癌症中心成员提供良好的服务 综合配套服务。2004年，细胞生物学和显微注射服务部与 形成生殖科学的生殖技术资源。合并所产生的协同效应 使管理层和工作人员能够确定集团内部的互补和重叠职能； 建立新的协作服务产品；合并类似流程以消除冗余；以及 将实验室、隔间和办公空间结合在一起，最大限度地提高工作效率。细胞生物学与微量注射 服务人员与生殖科学和分子生物学的其他成员密切合作 为癌症中心研究人员提供无缝访问服务的服务。这种密切的内部协作 确保拥有这些领域以外专业知识的调查人员能够充分利用当前的转基因和 基因打靶技术在自己的工作中通过利用这些互动服务。高级职员科学家Dr。 年，Thomas Gridley被任命为细胞生物学和显微注射服务的学院顾问(项目负责人) 1999年。他负责监督这个收费业务，雇佣了两名胚胎干细胞技术专家，两名 微注射技术员，一名菌落经理和高级运营经理。该服务占据了 研究实验室4号楼的1,899平方英尺的实验室、隔间和办公空间以及遗传学 资源建设。Gridley博士与癌症中心用户、服务人员和中心进行交流 行政管理，以确保以最有效率、最具成本效益和最有技术的方式满足研究需求 目前的方式。