CELL BIOLOGY/MICROINJECTION

生殖科学的细胞生物学和显微注射组件的使命是为癌症提供 中心成员可以使用中心设施、仪器和技术专长， 允许研究人员进行胚胎干（ES）细胞系衍生，电穿孔，并创建 用于体内基因功能研究的转基因小鼠。该服务产生转基因 小鼠的各种近交系和杂交背景，并已衍生生殖系感受态ES细胞 近交系小鼠品系。种系能力验证、培训、定制项目和测试 还提供试剂和用品。该服务以前曾得到癌症组织的支持， 中心支持补助金（CCSG）资金，与冷冻保存，进口和再衍生密切合作 在生殖科学，以及表型科学，为癌症中心成员提供良好的 一体化综合服务。2004年，细胞生物学和显微注射服务与 生殖技术资源形成生殖科学。合并所产生的协同效应 使管理层和工作人员能够确定小组内的互补和重叠职能; 建立新的协作服务;合并类似的流程以消除冗余;以及 联合收割机结合实验室、动物饲养场和办公空间，以最大限度地提高生产力。细胞生物学和显微注射 服务人员与生殖科学和分子生物学的其他成员密切合作 为癌症中心研究人员提供无缝访问服务的服务。这种紧密的内部合作 确保具有这些领域以外专业知识的研究人员能够充分利用当前的转基因技术， 基因打靶技术在他们自己的工作中通过利用这些互动服务。高级科学家Dr. 年，托马斯·格里德利被任命为细胞生物学和显微注射服务的教员顾问（项目负责人）。 1999.他监督这项收费服务的运作，雇用了两名胚胎干细胞技术专家， 显微注射技术专家，一个殖民地经理和高级运营经理。服务占据 位于研究实验室4号楼和遗传学实验室的1，899平方英尺的实验室、饲养室和办公空间 资源大厦。Gridley博士与癌症中心用户、服务人员和中心进行沟通 行政管理，以确保以最有效率、最具成本效益和技术 目前的方式。

The mission of the Cell Biology & Microinjection component of Reproductive Sciences is to provide Cancer Center members with access to centralized facilities, instrumentation and technical expertise necessary to allow investigators to carry out embryonic stem (ES) cell line derivation, electroporation, and to create genetically modified mice for in vivo studies of gene function. The Service generates genetically modified mice on a variety of inbred strain and hybrid backgrounds and has derived germline-competent ES cell lines from inbred mouse strains. Germline-competence validation, training, custom projects, and tested reagents and supplies are also offered. The Service, which has previously been supported by Cancer Center support grant (CCSG) funds, works closely with Cryopreservation, Importation and Rederivation within Reproductive Sciences, as well as Phenotyping Sciences to provide Cancer Center members well integrated comprehensive services. In 2004 the Cell Biology & Microinjection Service merged with the Reproductive Technologies Resource to form Reproductive Sciences. The synergy created by the merger allowed management and staff to identify complementary and overlapping functions within the groups; establish new collaborative service offerings; merge similar processes to eliminate redundancy; and combine laboratory, vivarium and office space to maximize productivity. Cell Biology & Microinjection Service personnel work closely with other members of Reproductive Sciences and the Molecular Biology Service to offer Cancer Center investigators seamless access to services. This close internal collaboration insures that investigators with expertise outside of these areas can fully employ current transgenic and gene targeting technology in their own work by utilizing these interactive services. Senior Staff Scientist Dr. Thomas Gridley was named Faculty Advisor (Project Leader) of Cell Biology & Microinjection Service in 1999. He oversees this fee-for-service operation which employs two ES cell technologists, two microinjection technologists, a colony manager and senior operational manager. The Service occupies 1,899 ft2 of laboratory, vivarium and office space in Research Laboratory Building 4 and the Genetics Resources Building. Dr. Gridley communicates with the Cancer Center users, Service staff and Center Administration to ensure that research needs are met in the most efficient, cost-effective and technically current manner.