ANIMAL MODELS FOR SAETHRE CHOTZEN SYNDROME

该项目的长期目标是生成和表征人类颅面疾病综合征的小鼠模型，并了解这些疾病综合征背后的遗传和生化途径。颅缝闭合症，即颅骨颅骨过早融合，是一个严重的医学问题，每3000个活产儿中就有1个发生。颅缝闭闭引起的颅骨生长异常可导致颅内压升高、脑血流受损、气道阻塞、视力和听力受损、学习困难和不良心理影响。在这项提议中，我们将研究sae3 - chotzen综合征的小鼠模型，sae3 - chotzen综合征是人类最常见的常染色体显性疾病之一。编码bhlh型转录因子的人类TWIST基因的单倍性不足已被证明是导致saethree - chotzen综合征的主要原因之一。saethree - chotzen综合征的其他家族性病例是由编码成纤维细胞生长因子受体（FGR2和FGR3）的一些基因突变引起的。小鼠Twist基因的零突变导致纯合子的早期胚胎死亡，而在杂合子中导致部分渗透性骨骼缺陷，复制saethree - chotzen综合征的某些特征。人类TWIST基因是果蝇TWIST基因的同源物。果蝇的遗传证据表明，Twist基因的突变与蜗牛基因编码的另一个转录因子的突变相互作用。我们在蜗牛的两个小鼠同源物（称为Sna和Slug）中构建了靶向突变。我们将验证一个假设，即在果蝇中，蜗牛和扭曲家族的基因在小鼠中都在相同的遗传途径中起作用。本建议的具体目的是：1。通过检测蜗牛家族基因Sna和Slug的表达变化，进一步表征Twist突变胚胎。同时检查其他基因（成纤维细胞生长因子受体和Msx基因）的表达是否在Twist突变胚胎中发生改变。2. 通过产生和分析Sna/Twist双突变体来验证假设，就像在果蝇中一样，Twist和Snail家族的基因将在相同的遗传途径中起作用。3. 产生并分析母鼠蜗牛家族基因Slug的双突变体（Slug/Twist双突变体）。

The long term goals of this project are to generate and characterize mouse models of human craniofacial disease syndromes, and to understand the genetic and biochemical pathways underlying these disease syndromes. Craniosynostosis, the premature fusion of the calvarial bones of the skull, is a significant medical problem, occurring in 1 in 3000 live births. The abnormal skull growth associated with craniosynostosis may result in raised intracranial pressure, impaired cerebral blood flow, airway obstruction, impaired vision and hearing learning difficulties and adverse psychological effects. In this proposal, we will study a mouse model for Saethre-Chotzen Syndrome, one of the most common autosomal dominant disorders of craniosynostosis in humans. Haploinsufficiency for the human TWIST gene, which encodes a bHLH-type transcription factor, has been demonstrated to be one of the major causes of Saethre-Chotzen Syndrome. Other familial cases of Saethre-Chotzen Syndrome are caused by mutations in some of the genes encoding fibroblast growth factor receptors (FGR2 and FGR3). A null mutation in the mouse Twist gene results in early embryonic death in homozygotes, and in heterozygotes results in partially penetrant skeletal defects that replicate certain features of Saethre-Chotzen syndrome. The human TWIST gene is a homolog of the Twist gene of Drosophila. Genetic evidence in Drosophila has demonstrated that mutations in the Twist gene interact with mutations in another transcription factor encoded by the Snail gene. We have constructed targeted mutations in two mouse homologs of Snail (termed Sna and Slug). We will test the hypothesis that, as in Drosophila, genes of the Snail and Twist family both function in the same genetic pathway in mice. The specific aims of this proposal are to: 1. Further characterize Twist mutant embryos by testing fro altered expression of the Snail family genes Sna and Slug. Also examine whether expression of other genes responsible for inherited craniosynostosis syndromes (the fibroblast growth factor receptors and the Msx genes) are altered in Twist mutant embryos. 2. Test the hypothesis that, as in Drosophila, genes of the Twist and Snail families will function in the same genetic pathway by generating and analyzing Sna/Twist double mutants. 3. Generate and analyze double mutants with the mother mouse Snail family gene Slug (Slug/Twist double mutants).