Differentiation of taste papilla epithelium during development

发育过程中味乳头上皮的分化

• 批准号: 8014910
• 负责人: Hongxiang Liu
• 金额: $ 14.54万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-31 至 2012-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8014910
• 关键词: Afferent Neurons; Apical; Appearance; Area; Attention; Biology; Cell Differentiation process; Cell Lineage; Cell Maintenance; Cells; Data; Derivation procedure; Development; Embryo; Epithelial Cells; Epithelium; Esthesia; Event; Feeding behaviors; Fiber; Foundations; Fungiform Papilla; Future; Goals; Grant; Housing; Immunohistochemistry; In Vitro; Indium; Knowledge; Label; Laboratories; LacZ Genes; Life; Location; Mesenchyme; Mus; Nature; Neural Crest; Neural Crest Cell; Neurons; Newborn Infant; Organ; Organ Culture Techniques; Peripheral; Phenotype; Publishing; Quality of life; Rattus; Reporter; Rodent; Sensory; Sensory Ganglia; Signal Transduction; Staging; Stimulus; System; Taste Bud Cell; Taste Buds; Taste Perception; Time; Tongue; Transgenes; Transgenic Mice; Tubulin; Vertebrates; base; blastomere structure; cell type; density; immunoreaction; insight; postnatal; precursor cell; public health relevance; relating to nervous system; research study; tongue papilla

DESCRIPTION (provided by applicant): Taste buds, the sensory end organs that transduce gustatory stimuli into neural signals, reside in taste papillae in the mammalian tongue. Therefore, taste papillae incorporate the epithelium that will differentiate to include cells of taste bud. However, the field of taste biology lacks a clear understanding of what constitutes possible taste cell precursors in developing papillae. In fact, there is no detailed information about cell specification of the early lingual epithelium that can cross-signal to underlying mesenchyme in papilla differentiation. The goals of this proposal are to characterize: (1) the localization and timing for neuronal, glial and taste cell phenotypes in embryonic and early postnatal tongue epithelium; (2) stage and location-specific distribution and phenotypic specification of neural crest derived cells (NCDCs) in developing lingual epithelium, mesenchyme and taste papillae. Immunohistochemistry on whole tongue and sections will be used to characterize and localize neuronal, glial and taste cell phenotypes, in embryonic and postnatal tongue epithelium and mesenchyme. Also, an in vitro, tongue organ culture system at select embryonic stages will be used to study whether cell types can develop and be sustained in the absence of an intact sensory contribution. We will use transgenic mouse lines with a lacZ (R26R) reporter or ZEG (lacZ/EGFP) double reporter transgene modulated by Wnt1- Cre or P0-Cre to label Wnt1- and P0-expressing NCDCs. The distribution and cell phenotypes of NCDCs in the developing tongue and taste papillae will be characterized. The results will bring new information to the field and provide an important foundation for understanding the derivation and progressive differentiation of developing taste papilla epithelium. Taste sensation, which is tranduced by taste buds in the gustatory papillae, guides nutritive choices and ingestive behaviors of all vertebrates. Thus a thorough knowledge of how taste papillae develop and differentiate to eventually include taste bud cells will contribute to better understanding how taste sensation develops. Public Health Relevance: The sense of taste informs our nutritive choices, which are essential for life and quality of life. Knowledge of taste papilla development will contribute to understanding how the distribution and density of tongue taste organs are determined, and therefore, to understanding how taste sensitivity emerges.

描述（由申请人提供）：味蕾是将味觉刺激转化为神经信号的感觉末端器官，位于哺乳动物舌头的味觉乳头中。因此，味乳头包含上皮细胞，其将分化为包括味蕾细胞。然而，味觉生物学领域缺乏一个明确的理解，什么构成可能的味觉细胞前体在发展中的乳头。事实上，没有关于早期舌上皮细胞特异性的详细信息，其可以在乳头分化中与基础间充质交叉信号。本研究的目的是：（1）胚胎和出生后早期舌上皮中神经元、神经胶质和味觉细胞表型的定位和时间;（2）发育中舌上皮、间充质和味觉乳头中神经嵴衍生细胞（NCDC）的阶段和位置特异性分布和表型特异性。将使用全舌和切片的免疫组织化学来表征和定位胚胎和出生后舌上皮和间充质中的神经元、神经胶质和味细胞表型。此外，在体外，舌器官培养系统在选定的胚胎阶段将被用来研究细胞类型是否可以发展和维持在没有完整的感觉贡献。我们将使用转基因小鼠系与lacZ（R26 R）报告基因或ZEG（lacZ/EGFP）双报告基因转基因Wnt 1- Cre或P0-Cre的调节标记Wnt 1-和P0-表达的NCDC。NCDC在发育中的舌和味觉乳头中的分布和细胞表型将被表征。本研究结果将为该领域的研究提供新的信息，并为了解发育中的味觉乳头上皮的起源和进行性分化提供重要的基础。味觉由味觉乳头中的味蕾传导，指导所有脊椎动物的营养选择和摄食行为。因此，深入了解味觉乳头如何发育和分化，最终包括味蕾细胞将有助于更好地理解味觉如何发展。 公共卫生相关性：味觉告知我们的营养选择，这对生活和生活质量至关重要。味觉乳头发育的知识将有助于理解舌头味觉器官的分布和密度是如何确定的，因此，理解味觉敏感性是如何出现的。