Molecular Basis of PII function

描述(申请人提供)：GlnB及其同系物是PII蛋白家族的成员，是几乎所有原核细胞的中央代谢调节因子，包括所有主要病原体。人们普遍认为，GlnB样蛋白感知细胞中的碳-氮平衡，并通过两种形式的混合做出反应：一种形式表示氮过剩，另一种形式表示氮限制。然后，这两种形式通过与细胞中的许多受体蛋白直接的蛋白质-蛋白质相互作用来影响各种细胞过程。最后，解决了氮过量形式的一些结构。尽管了解了这一点，但一些非常核心的问题仍然没有得到解决，这些问题是本提案的重点。首先，很明显，GlnB样蛋白与ATP结合，但这种ATP结合并不被认为具有生理意义。然而，强有力的初步证据表明，ATP水平的变化会影响细胞中GlnB的功能，这意味着GlnB整合了碳、氮和能量信号。研究能量状态对GlnB功能的作用是这项提议的第一个目的。AIM II的重点是更好地了解目前未知的GlnB氮限制形式的结构。初步证据表明，它与已知的氮过量形式的结构有戏剧性的不同。核磁共振和X射线结晶学的直接结构分析将得到构象变化的GlnB变体的存在的补充。这一目标也直接测试了最近的1-酮戊二酸结合位点模型，这是实现信号氮限制的形式所必需的，通过遗传和生化方法的组合。最后，AIM III讨论了GlnB与AmtB的相互作用，AmtB是一种完整的膜蛋白，也是氨气通道。这种相互作用的一个重要结果是直接影响细胞中的GlnB水平，从而影响GlnB与其他蛋白质相互作用的能力。生化生理分析的组合将确定这种GlnB-AmtB相互作用调节的性质和重要性。所有这些目标都有很高的成功可能性，因为必要的蛋白质变体已经掌握在手中，生化和遗传工具也是可用的。拟议的实验结果将极大地改善对所有生物体中的GlnB同系物如何在分子水平上整合氮、碳和能量状态的信号的描述。这个项目解决了几乎所有原核生物中发现的PII蛋白系统对碳、氮和能量感知的交集问题。由于其中心性，它直接或间接影响有益的(抗生素生产)和有害的(毒素生产)微生物过程。因此，这些结果将有助于解释有益生物体和病原生物体是如何感知环境并做出反应的。

DESCRIPTION (provided by applicant): GlnB and its homologs are members of the PII protein family and are central metabolic regulators in almost all prokaryotic cells, including all major pathogens. It is generally agreed that GlnB-like proteins sense the carbon-nitrogen balance in the cell and respond by assuming a mixture of two forms: one form signals nitrogen excess and the other signals nitrogen limitation. These two forms then affect a variety of cellular process by direct protein-protein interactions with a number of receptor proteins in the cell. Finally, a number of structures of the nitrogen-excess form have been solved. Despite this knowledge, some very central issues remain unresolved and are the focus of this proposal. First, it is clear that GlnB-like proteins bind ATP, but this ATP-binding has not been thought to be of physiological importance. However, strong preliminary evidence suggests that changes in ATP levels affect GlnB function in the cell, which implies that GlnB integrates carbon, nitrogen and energy signals. Examining the role of energy status on GlnB function is the first aim of this proposal. The focus of Aim II is to better understand the currently unknown structure of the nitrogen-limitation form of GlnB. Preliminary evidence suggests that it differs in dramatic ways from the known structures of the nitrogen-excess form. Direct structural analysis by NMR and X-ray crystallography will be complemented by the existence of conformationally altered GlnB variants. This aim also directly tests the recent model of the binding site of 1-ketoglutarate, which is necessary for achieving the form signaling nitrogen limitation, by a combination of genetic and biochemical approaches. Finally, Aim III addresses the interaction of GlnB with AmtB, an integral membrane protein that also serves as an ammonia gas channel. An important result of this interaction is to directly affect GlnB levels in the cell and therefore GlnB's ability to interact with other proteins. A combination of biochemical physiological assays will determine the nature and importance of the regulation of this GlnB-AmtB interaction. All these aims have a very high likelihood of success because the necessary protein variants are already in hand and the biochemical and genetic tools are available. The result of the proposed experiments will be a dramatically improved description of how GlnB homologs in all organisms integrate signals of nitrogen, carbon and energy status at the molecular level. This project addresses the intersection of carbon, nitrogen and energy sensing by the PII protein system found in almost all prokaryotes. Because of its centrality, it directly or indirectly affects both beneficial (antibiotic production) and harmful (toxin production) microbial processes. The results will therefore help explain how both beneficial and pathogenic organisms sense and respond to their environment.