Influences of the prenatal environment on metabolic programming

产前环境对代谢程序的影响

• 批准号: 7754852
• 负责人: KELLIE L. K. TAMASHIRO
• 金额: $ 24.65万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-10-01 至 2012-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7754852
• 关键词: Adolescence; Adult; Animal Model; Behavioral; Cardiovascular Diseases; Characteristics; Clinical; Consumption; DNA Methylation; DNA Methylation Regulation; Data; Development; Diabetes Mellitus; Diet; Disease; Endocrine; Environment; Epidemiology; Epigenetic Process; Etiology; Exposure to; Fatty acid glycerol esters; Funding; Genes; Goals; Health; Homeostasis; Human; Hypertension; Insulin Resistance; Intervention; Lactation; Lead; Life Style; Metabolic; Metabolic Diseases; Modification; Neuropeptides; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Personal Satisfaction; Phenotype; Physiological; Play; Prevention strategy; Public Health; Rattus; Risk; Rodent Model; Role; Stress; System; Testing; Time; Work; effective therapy; neuropsychiatry; nutrition; offspring; prenatal; prenatal influence; prenatal stress; programs; research study; response

Obesity Is a major public health problem worldwide and recent work has suggested that exposure to a suboptimal eariy environment may increase the risk of becoming obese. Epidemiological data show that an unfavorable intrauterine environment has long-term consequences in offspring including hypertension, cardiovascular disease, type 2 diabetes, obesity and neuropsychiatric disease. Specifically, prenatal stress and/or consumption of a high fat dieL characteristics of modern day human lifestyle, have been shown to lead to metabolic disorders such as obesity and Insulin resistance in offspring. However, the mechanisms involved are not well understood. The overall goal of this proposal is to characterize the short- and long-tenn effects of changes in the prenatal environment - stress and nutrition - on the behavioral and physiological development of offspring and to explore the possible neuropeptide and epigenetic mechanisms involved using a rat animal model. Specific aims are: 1) To detemiine the developmental time course of behavioral and endocrine alterations resulting from prenatal stress. We will also test the hypothesis that prenatal stress will accentuate diet-induced obesity, Timepoints during lactation, adolescence, and adulthood will be examined to characterize the phenotype and to direct examination of possible mechanisms; 2) To test the hypothesis that prenatal stress, high fat diet, or both result in alterations in neuropeptide systems regulating energy homeostasis that are consistent with other rodent models of obesity; and 3) To test the hypothesis that prenatal stress and nutrition results in obesity in offspring through epigenetic modifications via differential DNA methylation of genes that are critical to energy homeostasis. These experiments will enhance our understanding of the etiology of obesity and metabolic disease ultimately allowing the development of rational clinical interventions for such conditions.

肥胖是全球一个主要的公共卫生问题，最近的工作表明，接触 次优的环境可能会增加肥胖的风险。流行病学数据表明 不利的子宫内环境在包括高血压的后代有长期后果， 心血管疾病，2型糖尿病，肥胖和神经精神病。具体而言，产前应力 和/或消耗现代人类生活方式的高脂diel特征 导致后代肥胖和胰岛素抵抗等代谢疾病。但是，机制 涉及的理解不太了解。该提议的总体目标是表征短期和长期的 产前环境变化的影响 - 压力和营养对行为和生理的影响 后代的发展并探索可能涉及的神经肽和表观遗传机制 使用大鼠动物模型。具体目的是：1）逐渐忽略行为的发展时间过程 和由产前压力引起的内分泌改变。我们还将测试产前应激的假设 会强调饮食引起的肥胖症，泌乳期间的时间点，青春期和成年 检查表征表型并直接检查可能的机制； 2）测试 假设产前压力，高脂肪饮食或两者都会导致调节神经肽系统的改变 能量稳态与肥胖的其他啮齿动物模型一致； 3）检验假设 产前压力和营养通过表观遗传修饰通过 对能量稳态至关重要的基因的差异DNA甲基化。这些实验会 加强我们对肥胖症和代谢疾病的病因的理解，最终允许 开发这种情况的理性临床干预措施。