In-Focus Phase Contrast for Cryo-EM

用于冷冻电镜的聚焦相差

• 批准号: 8184089
• 负责人: KENNETH H DOWNING
• 金额: $ 65.51万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8184089
• 关键词: 3-Dimensional; Address; Binding; Biochemistry; Biological; Biological Testing; Biomedical Research; C-terminal; Cells; Cellular biology; Charge; Chemicals; Complex; Computers; Data Quality; Data Set; Dependence; Development; Device Designs; Devices; Due Process; Effectiveness; Electrodes; Electron Microscope; Electron Microscopy; Electrons; Electrostatics; Evaluation; Exhibits; Fourier Transform; Frequencies; Goals; Grant; Heterogeneity; High temperature of physical object; Hybrids; Ice; Image; Ions; Laboratories; Lasers; Learning; Medical Research; Methods; Microtubules; Modeling; Molecular; Molecular Conformation; Molecular Weight; Noise; Phase; Proteins; Radial; Relative (related person); Research; Research Project Grants; Resolution; Rest; Sampling; Signal Transduction; Specimen; Streptavidin; Structure; Sulfite reductase; Surface; Tail; Technology; Testing; Time; Tobacco Mosaic Virus; Tomogram; Tongue; Tube; Tubulin; Use Effectiveness; Work; base; design; flexibility; genetic regulatory protein; improved; macromolecular assembly; monolayer; novel; particle; protein complex; reconstruction; structural biology; tomography; tool; transmission process; voltage

DESCRIPTION (provided by applicant): The goal of the proposed research is to determine the effectiveness of using charging-free versions of two fundamentally different types of microfabricated phase-contrast devices, designed for use in the transmission electron microscope. The advantages of using in-focus phase contrast for applications in biomedical research are expected to be: Images of protein complexes as small as 200 kDa should be more easily identified and selected for computer processing, due to the improved contrast transfer function (CTF) at low spatial frequencies that is provided by in-focus phase contrast. Structural information at high resolution will no longer be corrupted or lost due to use of large values of defocus to achieve the needed amount of phase contrast. The improved image quality may result in improvement in the ability to detect structural (compositional) and conformational differences amongst particles in a structurally non-homogeneous sample. One type of device that will be evaluated is a microfabricated aperture that blocks a half-plane of the electron wave that is scattered at small angle, while at the same time allowing the rest of the scattered wave to contribute in the usual way to image formation. The second type of device is a novel, 2-electrode "electrostatic drift tube" design that applies a 90 degree phase shift to the unscattered electrons relative to the scattered electrons. Our fabrication of charging-free devices will employ high-temperature annealing and refinement of materials and devices, chemical and physical cleaning of surfaces, and the use of sensitive tools for detecting surface contamination. Fabrication methods will include traditional lithographic wet-fabrication as well as laser micromachining and focused-ion beam milling. The effectiveness of these devices will be determined from cryo-EM images of biological test specimens such as monolayer crystals of streptavidin and tobacco mosaic virus. Applications to current research will include studies on structural plasticity of microtubules and the interactions of various regulatory proteins with microtubules. PUBLIC HEALTH RELEVANCE: It is expected that our research will greatly improve the usefulness of electron microscopy of unstained, ice-embedded specimens (cryo-EM) for basic bio-medical research in the fields of biochemistry and cell biology. The improvement in image contrast that will be achieved will greatly increase the information that one can derive from images of complex protein assemblies and macromolecular machines. It may also make it possible to identify a much greater number of such molecular complexes in three-dimensional electron tomograms of whole cells.

描述（由申请人提供）：拟议研究的目标是确定使用两种基本不同类型的微制造相衬器件的免充电版本的有效性，该器件设计用于透射电子显微镜。在生物医学研究中使用聚焦相衬的优点预计是：小到200 kDa的蛋白质复合物的图像应该更容易识别和选择用于计算机处理，由于在低空间频率下的对比度传递函数（CTF）的改进，这是由聚焦相衬提供的。 由于使用大的散焦值来实现所需的相位对比度，高分辨率的结构信息将不再被破坏或丢失。 改进的图像质量可以导致检测结构非均匀样品中的颗粒之间的结构（组成）和构象差异的能力的改进。将被评估的一种类型的设备是一个微制造的孔径，它阻止了以小角度散射的电子波的半平面，同时允许其余的散射波以通常的方式有助于成像。第二种类型的器件是一种新颖的2电极“静电漂移管”设计，其相对于散射电子向未散射电子施加90度相移。我们的免充电设备的制造将采用高温退火和材料和设备的细化，表面的化学和物理清洁，以及使用敏感的工具来检测表面污染。制造方法将包括传统的光刻湿法制造以及激光微加工和聚焦离子束铣削。这些装置的有效性将通过生物测试样本（如链霉亲和素和烟草花叶病毒的单层晶体）的冷冻EM图像来确定。应用到当前的研究将包括微管的结构可塑性和各种调控蛋白与微管的相互作用的研究。 公共卫生关系：预计我们的研究将大大提高电子显微镜的有用性未染色，冰包埋标本（冷冻EM）的生物化学和细胞生物学领域的基础生物医学研究。将实现的图像对比度的改进将大大增加人们可以从复杂蛋白质组装和大分子机器的图像中获得的信息。它还可以在整个细胞的三维电子断层扫描图中识别出更多数量的这种分子复合物。