Novel Reagents and Devices for the Rapid Detection of Fire Retardant Chemicals.

用于快速检测阻燃化学品的新型试剂和设备。

• 批准号: 8074913
• 负责人: Ki-Chang Ahn
• 金额: $ 33.93万
• 依托单位: AGRI-ANALYSIS, LLC
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8074913
• 关键词: Adult; Air; Antibodies; Antigens; Binding; Biological Assay; Biotin; California; Chemicals; Chemistry; Child; Collaborations; Color; Communication; Complex; Computer software; Cyclic Peptides; Data; Data Collection; Detection; Devices; Electronics; Engineering; Environment; Environmental Pollution; Enzyme-Linked Immunosorbent Assay; Estrogens; Europe; Exposure to; Fatty acid glycerol esters; Fire - disasters; Flame Retardants; Fluorescence; Fluorescence Polarization; Food Supply; Furniture; Germany; Haptens; Home environment; Hormones; Human; Human Milk; Immunoassay; Japan; Korea; Label; Legal patent; Mattresses; Measurement; Methods; Molecular; Monitor; Nervous System Trauma; One-Step dentin bonding system; Peptides; Process; Reader; Reagent; Regulation; Reproductive system; Risk; Sampling; Screening procedure; Serum; Services; Signal Transduction; Site; Streptavidin; System; Technology; Testing; Textiles; Thyroid Hormones; Time; Tissues; Toy; Tracer; United States; Universities; Utah; Validation; Water Supply; Wireless Technology; Work; assay development; base; design; experience; fluorophore; innovation; liquid chromatography mass spectrometry; meetings; novel; phenyl ether; programs; public health relevance; rapid detection; sample collection

DESCRIPTION (provided by applicant): To meet the Agency's requirement for rapid, precise and on-site measurement of emerging contaminants such as fire retardant chemical PBDEs, critical technologies must be developed where upon mixing a reagent and a detection target, a signal is generated without the need of washing and separation steps required by existing methods. The objective of this project is to develop innovative fluorescent reagents and field- deployable devices for BDE-47 because fluorescence is among the most sensitive detection method, and BDE-47 is a wide spread congener of PBDEs. Current testing methods for PBDEs are predominantly GC/LC/MS and ELISA kits. They take multiple steps and require skilled operators, are tedious, expensive, and not suitable for field use. We plan to accomplish this objective by designing and validating several homogenous immunoassays which produce a concurrent change in fluorescence intensity in the presence of BDE-47. Specifically, we plan to identify tracer antigens for competitive format for applications that do not require high sensitivity (> ppb). For applications that require higher sensitivity (< ppb), we plan to use novel small cyclic peptides that bind to the BDE-47/antibody immunocomplex to develop sensitive sandwich assays It is within project deliverable to design and build portable fluorescence readers with easy sample collection and with wireless data capability for real-time data collection. An alternative deliverable includes rapid dipstick testing devices to be fabricated and tested for BDE-47. PUBLIC HEALTH RELEVANCE: Polybrominated diphenyl ethers (PBDEs) are common flame retardants used to reduce the risk of fire in a wide variety of products, such as mattresses, furniture, home electronics, textiles and children's toys. While they are excellent flame retardants, PBDEs enter our body through contaminated air, water and food supply. They bioaccumulate in human milk, serum and fat tissues. Scientific studies have concluded that regular exposure to even low levels of these chemicals may irreparably damage the nervous and reproductive systems, and disrupt hormones such as estrogen and thyroid hormones. In United States, PBDE concentrations in adults are 10-100 times higher than the 1-3 ppb levels found in Europe or Japan. Some studies have found concentrations of the lower-brominated PBDEs were 2- to 5-fold higher in children than in adults. Some predict PBDE body concentrations to exponentially increase doubling every 4 to 5 years. Given its wide spread use in our daily lives, effective control and elimination will be a long and challenging process. We believe that rapid and precise monitoring must be an integral part of any control program or regulation. Existing detection methods require experienced operators and take a long time, are therefore tedious, expensive, not suitable for field use. This project proposes to bridge this gap by using the state-of-the-art detection technologies to make portable devices that are field-deployable for rapid and precise measurement in humans as well as in environment.

描述（由申请人提供）：为了满足原子能机构对阻燃化学品多溴二苯醚等新出现的污染物进行快速、精确和现场测量的要求，必须开发关键技术，在将试剂和检测目标混合后，无需现有方法所需的洗涤和分离步骤即可产生信号。该项目的目标是开发BDE-47的创新荧光试剂和可现场部署的装置，因为荧光是最灵敏的检测方法之一，而BDE-47是一种广泛分布的多溴二苯醚同系物。目前对多溴二苯醚的检测方法主要是GC/LC/MS和ELISA试剂盒。它们需要多个步骤并且需要熟练的操作者，是繁琐的、昂贵的并且不适合现场使用。我们计划通过设计和验证几种在BDE-47存在下产生荧光强度同时变化的均相免疫测定来实现这一目标。具体来说，我们计划为不需要高灵敏度（> ppb）的应用识别竞争形式的示踪抗原。对于需要更高灵敏度（< ppb）的应用，我们计划使用与BDE-47/抗体免疫复合物结合的新型小环肽来开发灵敏的夹心测定法。另一项可交付成果包括有待制造和测试BDE-47的快速试纸测试装置。 公共卫生相关性：多溴联苯醚（PBDEs）是一种常见的阻燃剂，用于降低床垫、家具、家用电子产品、纺织品和儿童玩具等各种产品的火灾风险。虽然多溴联苯醚是极好的阻燃剂，但它们通过受污染的空气、水和食物进入我们的身体。它们在人乳、血清和脂肪组织中生物累积。科学研究得出的结论是，经常接触这些化学物质，即使是低水平的，也可能会对神经和生殖系统造成不可挽回的损害，并破坏雌激素和甲状腺激素等激素。在美国，成人体内的多溴二苯醚浓度比欧洲或日本的1-3 ppb高出10-100倍。一些研究发现，儿童体内溴化程度较低的多溴二苯醚浓度比成人高2至5倍。有人预测，体内多溴二苯醚浓度每4至5年就会成倍增加。鉴于其在我们日常生活中的广泛使用，有效控制和消除将是一个长期和具有挑战性的过程。我们认为，快速和精确的监测必须是任何控制方案或法规的组成部分。现有的检测方法需要经验丰富的操作人员，耗时较长，因此繁琐、昂贵，不适合现场使用。该项目提出通过使用最先进的检测技术来弥补这一差距，使便携式设备可以现场部署，用于在人类和环境中进行快速精确的测量。