Qiagen PyroMark Q96 MD Automated Pyrosequencing System

Qiagen PyroMark Q96 MD 自动焦磷酸测序系统

• 批准号: 8052186
• 负责人: MARK T GROUDINE
• 金额: $ 14.2万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-23 至 2012-09-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052186
• 关键词: Address; Alleles; Area; Award; Biological; Disease; Ensure; Epigenetic Process; Fees; Fred Hutchinson Cancer Research Center; Funding; Future; Gene Mutation; Genetic Polymorphism; Genomics Shared Resource; Housing; Malignant Neoplasms; Mutate; Mutation; Other Genetics; Predisposition; Process; Request for Applications; Research; Research Infrastructure; Research Personnel; System; Tissues; United States National Institutes of Health; Validation; anticancer research; base; clinically relevant; instrument

DESCRIPTION (provided by applicant): This application requests funds to purchase a Qiagen PyroMark Q96 MD Automated Pyrosequencing System to support over 10 NIH-funded projects involving researchers at the Fred Hutchinson Cancer Research Center (FHCRC). These projects address important areas of cancer research that include the identification and validation of genotypic and epigenetic markers that have the potential to predict the predisposition to various cancers and other genetic-based diseases. Pyrosequencing offers an accurate means to rapidly assess the proportion of mutated or epigenetically altered alleles in tissues in a high throughput manner. This information is critical in determining the biological and clinical relevance of these gene alterations in disease processes. Currently, there is no such instrument or similar capabilities for assessing both genetic alterations or polymorphisms and epigenetic alterations available to researchers in the Seattle area. If awarded, the PyroMark Q96 MD would be housed in the FHCRC Genomics Shared Resource, where expertise exists to operate, maintain, and manage the instrument, and a fee-based infrastructure is in place to ensure long-term support. The requested instrument will address both immediate and future needs, supporting translational NIH-funded research at the FHCRC.

描述（由申请人提供）：本申请要求资金购买Qiagen PyroMark Q96 MD自动焦磷酸测序系统，以支持超过10个NIH资助的项目，涉及Fred哈钦森癌症研究中心（FHCRC）的研究人员。这些项目涉及癌症研究的重要领域，包括识别和验证基因型和表观遗传标记，这些标记有可能预测各种癌症和其他遗传疾病的易感性。焦磷酸测序提供了以高通量方式快速评估组织中突变或表观遗传改变的等位基因的比例的准确手段。这些信息对于确定这些基因改变在疾病过程中的生物学和临床相关性至关重要。目前，在西雅图地区的研究人员还没有这样的仪器或类似的能力来评估遗传改变或多态性和表观遗传改变。如果获得授权，PyroMark Q96 MD将被安置在FHCRC基因组学共享资源中，在那里有专业知识来操作，维护和管理仪器，并且有收费的基础设施来确保长期支持。所要求的工具将满足当前和未来的需求，支持FHCRC的NIH资助的翻译研究。

项目成果

On Causal Inferences for Personalized Medicine: How Hidden Causal Assumptions Led to Erroneous Causal Claims About the D-Value.

关于个性化医疗的因果推论：隐藏的因果假设如何导致关于 D 值的错误因果断言。

• DOI: 10.1080/00031305.2019.1575771
• 发表时间: 2020
• 期刊: The American statistician
• 作者: Greenland,Sander;Fay,MichaelP;Brittain,EricaH;Shih,JoannaH;Follmann,DeanA;Gabriel,ErinE;Robins,JamesM
• 通讯作者: Robins,JamesM