Transmission Electron Microscope

透射电子显微镜

• 批准号: 8050743
• 负责人: Judy Drazba
• 金额: $ 51.81万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8050743
• 关键词: 21 year old; Adult; Alzheimer' s Disease; Atherosclerosis; Atrial Fibrillation; Clinic; Communities; Congenital Abnormality; Contract Services; Disease; Electron Microscope; Electron Microscopy; Equipment; Film; Funding; Goals; Health; Image; Inflammatory; Investigation; Kidney Failure; Knowledge; Lead; Maintenance; Malignant Neoplasms; Manufacturer Name; Microscope; Microscopy; Pelvic Floor Disorders; Preparation; Prevention; Recording of previous events; Research; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Resources; Running; Sampling; Services; System; Time; United States National Institutes of Health; Wages; base; bioimaging; digital imaging; experience; instrument; notch protein; operation; pulmonary arterial hypertension; research facility; transmission process

DESCRIPTION (provided by applicant): The Imaging Core of the Cleveland Clinic Lerner Research Institute (LRI) and a group of seventeen NIH-funded investigators request funds to purchase a transmission electron microscope (TEM) - specifically the FEI Tecnai Spirit BioTwin. The Imaging Core was established sixteen years ago to provide leading-edge microscopy equipment and expertise for the Cleveland Clinic research community. The Core has a 21-year-old FEI CM12 TEM, used by 58 registered investigators from 18 departments. This instrument has served our researchers well, but has required more frequent service calls and was, in fact, declared obsolete by the manufacturer at the end of last year (and is no longer eligible for a full service contract). Besides the problems caused for research projects by equipment downtime, our film-based TEM is slow, unreliable, and expensive. The instrument we are requesting herein will provide a dependable resource for all of the basic capabilities of the existing TEM, but will also offer digital imaging. Although our Core provides a great array of top-notch microscopes for biomedical imaging, there are many NIH-funded research goals that can only be pursued with electron microscopy, making it essential for a facility such as ours to have an up-to-date instrument. Knowledge obtained from the projects highlighted here will hopefully lead to better modes of prevention and treatment for conditions such as inflammatory diseases, pelvic floor disorders, pulmonary arterial hypertension, cancer, kidney failure, Alzheimer's disease, atherosclerosis, atrial fibrillation, and birth defects. Placing the system in the Imaging Core - with its centrality in a significant research facility - will not only maximize its benefits for a variety of health-related investigations, but will assure its management by a skilled staff. Dr. Judith Drazba, LRI Imaging Core Director, has over twenty seven years electron microscopy experience, sixteen of them running this facility. A full-time EM technician, also with long experience, oversees the operation of the TEM and provides complete sample preparation. A Core manager also assists with user services and equipment troubleshooting and maintenance. Finally, the Lerner Research Institute which has a long history of strong support for centralized facilities continues its commitment to the Imaging Core by providing funds for salary support, service contracts, and infrastructure. PUBLIC HEALTH RELEVANCE: Use of the electron microscope proposed in this grant will give researchers the ability to produce highly magnified images of cells and tissues to see details on a much smaller scale than with light microscopes. Observing the ultrastructure of these samples leads to a better understanding of how they are organized and how they operate. With the knowledge obtained from use of this instrument, our investigators hope to discover better modes of prevention and treatment for conditions such as inflammatory diseases, pelvic floor disorders, pulmonary arterial hypertension, cancer, kidney failure, Alzheimer's disease, atherosclerosis, atrial fibrillation, and birth defects.

描述（由申请人提供）：克利夫兰诊所勒纳研究所（LRI）的成像核心和一组17名NIH资助的研究人员要求资金购买透射电子显微镜（TEM）-特别是FEI Tecnai Spirit BioTwin。成像核心成立于16年前，为克利夫兰诊所研究界提供领先的显微镜设备和专业知识。核心有一个21岁的FEI CM 12 TEM，由来自18个部门的58名注册调查员使用。该仪器为我们的研究人员提供了很好的服务，但需要更频繁的服务电话，事实上，制造商在去年年底宣布过时（并且不再有资格获得完整的服务合同）。除了设备停机给研究项目带来的问题外，我们的薄膜TEM速度慢、不可靠且价格昂贵。我们在此要求的仪器将为现有TEM的所有基本功能提供可靠的资源，但也将提供数字成像。虽然我们的核心为生物医学成像提供了大量一流的显微镜，但有许多NIH资助的研究目标只能用电子显微镜来实现，这使得像我们这样的设施必须拥有最新的仪器。从这里强调的项目中获得的知识有望导致更好的预防和治疗炎症性疾病、骨盆底疾病、肺动脉高压、癌症、肾衰竭、阿尔茨海默病、动脉粥样硬化、心房颤动和出生缺陷等疾病的模式。将该系统放置在成像核心中-其在重要研究设施中的中心地位-不仅可以最大限度地提高其对各种健康相关研究的益处，而且可以确保由熟练的工作人员进行管理。Judith Drazba博士，LRI成像核心主任，拥有超过27年的电子显微镜经验，其中16年运行该设施。一名经验丰富的全职EM技术员负责监督TEM的操作并提供完整的样品制备。核心经理还协助用户服务和设备故障排除和维护。最后，Lerner研究所长期以来一直大力支持集中式设施，通过提供工资支持、服务合同和基础设施资金，继续致力于成像核心。 公共卫生相关性：使用该资助中提议的电子显微镜将使研究人员能够产生高度放大的细胞和组织图像，从而以比光学显微镜小得多的规模查看细节。观察这些样品的超微结构可以更好地了解它们是如何组织的，以及它们是如何运作的。通过使用该仪器获得的知识，我们的研究人员希望发现更好的预防和治疗炎症性疾病、骨盆底疾病、肺动脉高压、癌症、肾衰竭、阿尔茨海默病、动脉粥样硬化、房颤和出生缺陷等疾病的模式。