Spinning Disk Confocoal Microscope

转盘共焦显微镜

• 批准号: 7792682
• 负责人: Judy Drazba
• 金额: $ 50万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2012-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792682
• 关键词: Actins; Adhesions; Area; B-Lymphocytes; Biomedical Research; Cell membrane; Cell physiology; Cells; Clinic; Communities; Coupled; Disseminated Malignant Neoplasm; Drosophila melanogaster; Endothelial Cells; Equipment; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Foxes; Funding; GTP-Binding Proteins; Gene Activation; Housing; Image; Lymphocyte Activation; Measurement; Microscope; Minor; Myosin Type II; Organism; Ovarian; Postdoctoral Fellow; Process; Proteins; Regulation; Research; Research Institute; Research Personnel; Resolution; Role; Running; Sampling; Signal Pathway; Slide; System; Tight Junctions; Time; Tissue Inhibitor of Metalloproteinase-3; Transcriptional Regulation; United States National Institutes of Health; Vascular Endothelial Growth Factors; Vesicle; angiogenesis; cancer cell; cell motility; cellular imaging; chromatin remodeling; digital imaging; experience; hedgehog signal transduction; human SMO protein; improved; instrument; migration; photoactivation; polymerization; trafficking

DESCRIPTION (provided by applicant): The Imaging Core of the Cleveland Clinic Lerner Research Institute and a group of fifteen NIH- funded investigators request funds to purchase a spinning disk confocal microscope system for live-cell imaging - specifically the Perkin Elmer Ultraview Vox System coupled to a Leica DMI6000 inverted microscope. The LRI Imaging Core was established fifteen years ago to provide leading-edge digital imaging equipment and expertise for the Cleveland Clinic research community. This facility will house and manage the requested instrument, making it available to the Cleveland area scientific community when not being used by the major and minor users. Dr. Judith Drazba, LRI Imaging Core Director, has over twenty years' confocal experience, fifteen of them running this facility. Three full-time technicians and a Research Associate assist her. The Core has two upright Leica SP2 confocal microscopes, heavily used by 122 current users from 24 departments. These instruments are limited to imaging fixed samples on slides. There is currently no spinning disk confocal microscope system anywhere in Cleveland. The requested instrument will offer researchers greatly improved temporal resolution needed to study dynamic cellular processes. It will also provide the ability to do fluorescence resonance energy transfer (FRET) measurements, fluorescence recovery after photobleaching (FRAP) measurements and photoactivation, thereby substantially advancing their NIH-funded biomedical research. Projects of the major users involve regulation of angiogenesis by TIMP-3 (Dr. Anand-Apte), signaling pathways responsible for assembly and localization of myosin II in metastatic cancer cells (Dr. Egelhoff), role of actin polymerization regulating cell polarization and migration during angiogenesis (Dr. Fox), regulation of B lymphocyte activation, proliferation, and motility by plasma membrane-actin cytoskeletal remodeling (Dr. Gupta), role of vesicle trafficking between tight junctions and the plasma membrane in the regulation of VEGF-driven directional migration of endothelial cells (Dr. Horowitz), the role of the G protein b2 subunit in the process of transcription regulation, gene activation and chromatin remodeling (Dr. Karnik), the role of Par-1 in regulating the localization of cytoskeletal and adhesion proteins during ovarian border cell migration in Drosophila melanogaster (Dr. McDonald), and the targeting and trafficking of Smoothened protein, an essential activator in Hedgehog signaling transduction (Dr. Zhu).

描述（由申请人提供）：Cleveland Clinic Lerner Research Institute的成像中心和一组由15名NIH资助的研究人员申请资金购买用于活细胞成像的旋转圆盘共聚焦显微镜系统-特别是Perkin Elmer Ultraview Vox系统，该系统与Leica DMI 6000倒置显微镜耦合。LRI成像核心成立于15年前，为克利夫兰诊所研究界提供领先的数字成像设备和专业知识。该设施将存放和管理所要求的仪器，使其在不被主要和次要用户使用时可供克利夫兰地区科学界使用。Judith Drazba博士，LRI成像核心主任，拥有超过二十年的共聚焦经验，其中十五年负责该设施。三名全职技术人员和一名研究助理协助她工作。Core配备了两台直立式Leica SP2共焦显微镜，目前有来自24个部门的122名用户在使用。这些仪器仅限于对载玻片上的固定样本进行成像。目前在克利夫兰的任何地方都没有旋转圆盘共聚焦显微镜系统。所要求的仪器将为研究人员提供研究动态细胞过程所需的大大提高的时间分辨率。它还将提供荧光共振能量转移（FRET）测量，光漂白（FRAP）测量和光活化后的荧光恢复的能力，从而大大推进他们的NIH资助的生物医学研究。主要用户的项目涉及TIMP-3对血管生成的调节（Anand-Apte博士），负责转移性癌细胞中肌球蛋白II组装和定位的信号通路（Egelhoff博士），肌动蛋白聚合在血管生成过程中调节细胞极化和迁移的作用（福克斯博士），调节B淋巴细胞的活化，增殖和运动的质膜肌动蛋白细胞骨架重塑（Gupta博士），紧密连接和质膜之间的囊泡运输在VEGF驱动的内皮细胞定向迁移调节中的作用（Dr. Horowitz），G蛋白b2亚基在转录调控、基因激活和染色质重塑过程中的作用（Karnik博士）、Par-1在调节黑腹果蝇卵巢边缘细胞迁移过程中细胞骨架和粘附蛋白定位中的作用（McDonald博士），以及Smoothened蛋白（Hedgehog信号传导中的必需激活剂）的靶向和运输（朱博士）。

项目成果

Alpha/Beta Interferon (IFN-α/β) Signaling in Astrocytes Mediates Protection against Viral Encephalomyelitis and Regulates IFN-γ-Dependent Responses.

• DOI: 10.1128/jvi.01901-17
• 发表时间: 2018-05-15
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Hwang M;Bergmann CC
• 通讯作者: Bergmann CC

Hyaluronan 35kDa treatment protects mice from Citrobacter rodentium infection and induces epithelial tight junction protein ZO-1 in vivo.

• DOI: 10.1016/j.matbio.2016.11.001
• 发表时间: 2017-10
• 期刊: Matrix biology : journal of the International Society for Matrix Biology
• 作者: Kim Y;Kessler SP;Obery DR;Homer CR;McDonald C;de la Motte CA
• 通讯作者: de la Motte CA

Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression.

• DOI: 10.1016/j.stem.2016.12.001
• 发表时间: 2017-04-06
• 期刊: Cell stem cell
• 影响因子: 23.9
• 作者: Alvarado AG;Thiagarajan PS;Mulkearns-Hubert EE;Silver DJ;Hale JS;Alban TJ;Turaga SM;Jarrar A;Reizes O;Longworth MS;Vogelbaum MA;Lathia JD
• 通讯作者: Lathia JD

Comparing and Contrasting the Effects of Drosophila Condensin II Subunit dCAP-D3 Overexpression and Depletion in Vivo.

• DOI: 10.1534/genetics.118.301344
• 发表时间: 2018-10
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Deutschman E;Ward JR;Ho-A-Lim KT;Alban TJ;Zhang D;Willard B;Lemieux ME;Lathia JD;Longworth MS
• 通讯作者: Longworth MS