Upgrade to E500 X- and Q-Band CW EPR Spectrometer for Biomedical Research

描述(由申请人提供)：这项提案寻求资金，以升级佛罗里达大学(UF)现有的电子顺磁共振(EPR)设备，用于生物医学研究。我们特别希望将我们的Bruker ESP300e X波段连续波(CW)EPR光谱仪升级到Bruker E500 X波段(9.5 GHz)和Q波段(35 GHz)连续波EPR光谱仪，其变温能力降至1.8K。拟议的升级将为UF和中佛罗里达大学(UCF)的几个研究小组提供高于我们现有仪器的新功能。这些功能包括用于金属蛋白EPR研究的1.8K温度能力和用于增强光谱分辨率的Q波段频率，以及通过天然非结构蛋白质和膜蛋白质的多频率方法研究蛋白质动力学。该仪器还将成为国家强磁场实验室(NHMFL)外部用户计划的一部分，并通过该计划向全世界的请求提供。此外，这台新的光谱仪将减轻目前在我们的单脉冲EPR光谱仪上进行的连续波EPR实验的负担。UF的十多个学院以及外部合作，以及UCF的两个学院将从此次升级提供的更长的EPR时间和能力中受益。化学系里有几个院系。许多人是医学院的用户或合作者；一些人是新教师。NIH资助的需要EPR实验的生物医学系统研究在我们的用户群中有所不同，包括对纳米颗粒、自旋捕获和剂量测量的研究以及在放射治疗和氧化损伤中的应用；对参与能量代谢的金属蛋白和无机复合体的表征；对调节细胞周期并参与肿瘤学的天然非结构蛋白的定点自旋标记(SDSL)研究；对参与脂质和营养转运的膜蛋白的SDSL研究；以及对天然多态和HIV-1蛋白酶耐药结构的构象采样的SDSL研究。

DESCRIPTION (provided by applicant): This proposal seeks funds to upgrade current electron paramagnetic resonance (EPR) equipment at the University of Florida (UF) for biomedical studies. We specifically wish to upgrade our Bruker ESP300e X-band continuous wave (CW) EPR spectrometer to a Bruker E500 X-band (9.5 GHz) and Q-band (35 GHz) CW EPR spectrometer with the variable temperature capabilities down to 1.8 K. The proposed upgrade will provide new capabilities above our current instrumentation to several research groups at UF and at the University of Central Florida (UCF). These include temperature capabilities to 1.8 K for metalloprotein EPR investigations and Q- band frequencies for enhanced spectral resolution and investigation of protein dynamics through a multi- frequency approach in natively unstructured proteins and membrane proteins. This instrument will also become part of the external users program at the National High Magnetic Field Laboratory (NHMFL) and be made available to requests world-wide through that program. In addition, this new spectrometer will relieve the burden of CW EPR experiments that are currently being performed on our single pulsed EPR spectrometer. Over ten Faculty at UF, along with outside collaborations, and two Faculty at UCF will benefit from the increased EPR time and capabilities offered by this upgrade. Several of the Faculty are within the Chemistry Department. Many are Users or collaborators from the College of Medicine; some are new faculty. The NIH funded research of biomedical systems requiring EPR experiments vary across our User base and include studies of nanoparticles, spin-trapping and dosimetry measurements with applications in radiation therapy and oxidative damage; characterization of metalloproteins and inorganic complexes involved in energy metabolism; site-directed spin- labeling (SDSL) studies of natively unstructured proteins that regulate cell cycle and are involved in oncology; SDSL studies of membrane proteins involved in lipid and nutrient transport, and SDSL studies of conformational sampling in natural polymorphisms and drug-resistant constructs of HIV-1 protease.