Upgrade to E500 X- and Q-Band CW EPR Spectrometer for Biomedical Research

描述（由申请人提供）：本提案寻求资金，以升级目前的电子顺磁共振（EPR）设备在佛罗里达大学（UF）的生物医学研究。我们特别希望将我们的Bruker ESP 300 e X波段连续波（CW）EPR光谱仪升级为Bruker E500 X波段（9.5 GHz）和Q波段（35 GHz）CW EPR光谱仪，其变温能力低至1.8 K。拟议的升级将提供新的能力，我们目前的仪器，在佛罗里达大学和中央佛罗里达大学（UCF）的几个研究小组。这些包括温度能力为1.8 K的金属蛋白质EPR调查和Q带频率的增强光谱分辨率和调查的蛋白质动力学通过多频率的方法在天然非结构化蛋白质和膜蛋白质。该仪器还将成为国家强磁场实验室（NHMFL）外部用户计划的一部分，并通过该计划向世界各地的要求提供。此外，这种新的光谱仪将减轻目前在我们的单脉冲EPR光谱仪上进行的CW EPR实验的负担。超过10个教师在UF，沿着与外部合作，并在UCF两个教师将受益于增加EPR时间和能力，通过这次升级提供。一些学院是在化学系。许多人是来自医学院的用户或合作者;有些是新教师。NIH资助的需要EPR实验的生物医学系统的研究在我们的用户群中各不相同，包括纳米颗粒的研究，自旋捕获和剂量测定测量，在放射治疗和氧化损伤中的应用;金属蛋白和参与能量代谢的无机复合物的表征;调节细胞周期和参与肿瘤学的天然非结构化蛋白质的定点自旋标记（SDSL）研究;涉及脂质和营养转运的膜蛋白的SDSL研究，以及HIV-1蛋白酶的天然多态性和耐药结构中构象取样的SDSL研究。

DESCRIPTION (provided by applicant): This proposal seeks funds to upgrade current electron paramagnetic resonance (EPR) equipment at the University of Florida (UF) for biomedical studies. We specifically wish to upgrade our Bruker ESP300e X-band continuous wave (CW) EPR spectrometer to a Bruker E500 X-band (9.5 GHz) and Q-band (35 GHz) CW EPR spectrometer with the variable temperature capabilities down to 1.8 K. The proposed upgrade will provide new capabilities above our current instrumentation to several research groups at UF and at the University of Central Florida (UCF). These include temperature capabilities to 1.8 K for metalloprotein EPR investigations and Q- band frequencies for enhanced spectral resolution and investigation of protein dynamics through a multi- frequency approach in natively unstructured proteins and membrane proteins. This instrument will also become part of the external users program at the National High Magnetic Field Laboratory (NHMFL) and be made available to requests world-wide through that program. In addition, this new spectrometer will relieve the burden of CW EPR experiments that are currently being performed on our single pulsed EPR spectrometer. Over ten Faculty at UF, along with outside collaborations, and two Faculty at UCF will benefit from the increased EPR time and capabilities offered by this upgrade. Several of the Faculty are within the Chemistry Department. Many are Users or collaborators from the College of Medicine; some are new faculty. The NIH funded research of biomedical systems requiring EPR experiments vary across our User base and include studies of nanoparticles, spin-trapping and dosimetry measurements with applications in radiation therapy and oxidative damage; characterization of metalloproteins and inorganic complexes involved in energy metabolism; site-directed spin- labeling (SDSL) studies of natively unstructured proteins that regulate cell cycle and are involved in oncology; SDSL studies of membrane proteins involved in lipid and nutrient transport, and SDSL studies of conformational sampling in natural polymorphisms and drug-resistant constructs of HIV-1 protease.