Upgrade to E500 X- and Q-Band CW EPR Spectrometer for Biomedical Research

升级至 E500 X 和 Q 波段 CW EPR 光谱仪用于生物医学研究

• 批准号: 8051278
• 负责人: GAIL E FANUCCI
• 金额: $ 52.3万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2013-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051278
• 关键词: Biomedical Research; Cell Cycle; Chemistry; Collaborations; Complex; Drug resistance; Electron Spin Resonance Spectroscopy; Energy Metabolism; Equipment; Faculty; Florida; Frequencies; Funding; Genetic Polymorphism; HIV-1; Investigation; Laboratories; Lipids; Measurement; Medicine; Membrane Proteins; Metalloproteins; Nutrient; Peptide Hydrolases; Physiologic pulse; Protein Dynamics; Proteins; Radiation therapy; Research; Resolution; Sampling; Site; Spin Labels; Spin Trapping; System; Temperature; Time; United States National Institutes of Health; Universities; base; college; dosimetry; instrument; instrumentation; magnetic field; nanoparticle; oncology; oxidative damage; programs; research study

DESCRIPTION (provided by applicant): This proposal seeks funds to upgrade current electron paramagnetic resonance (EPR) equipment at the University of Florida (UF) for biomedical studies. We specifically wish to upgrade our Bruker ESP300e X-band continuous wave (CW) EPR spectrometer to a Bruker E500 X-band (9.5 GHz) and Q-band (35 GHz) CW EPR spectrometer with the variable temperature capabilities down to 1.8 K. The proposed upgrade will provide new capabilities above our current instrumentation to several research groups at UF and at the University of Central Florida (UCF). These include temperature capabilities to 1.8 K for metalloprotein EPR investigations and Q- band frequencies for enhanced spectral resolution and investigation of protein dynamics through a multi- frequency approach in natively unstructured proteins and membrane proteins. This instrument will also become part of the external users program at the National High Magnetic Field Laboratory (NHMFL) and be made available to requests world-wide through that program. In addition, this new spectrometer will relieve the burden of CW EPR experiments that are currently being performed on our single pulsed EPR spectrometer. Over ten Faculty at UF, along with outside collaborations, and two Faculty at UCF will benefit from the increased EPR time and capabilities offered by this upgrade. Several of the Faculty are within the Chemistry Department. Many are Users or collaborators from the College of Medicine; some are new faculty. The NIH funded research of biomedical systems requiring EPR experiments vary across our User base and include studies of nanoparticles, spin-trapping and dosimetry measurements with applications in radiation therapy and oxidative damage; characterization of metalloproteins and inorganic complexes involved in energy metabolism; site-directed spin- labeling (SDSL) studies of natively unstructured proteins that regulate cell cycle and are involved in oncology; SDSL studies of membrane proteins involved in lipid and nutrient transport, and SDSL studies of conformational sampling in natural polymorphisms and drug-resistant constructs of HIV-1 protease.

描述（由申请人提供）：该提案寻求资金升级佛罗里达大学（UF）当前用于生物医学研究的电子顺磁共振（EPR）设备。我们特别希望将我们的 Bruker ESP300e X 波段连续波 (CW) EPR 光谱仪升级为 Bruker E500 X 波段 (9.5 GHz) 和 Q 波段 (35 GHz) CW EPR 光谱仪，其可变温度能力低至 1.8 K。拟议的升级将为佛罗里达大学和中佛罗里达大学 (UCF) 的几个研究小组提供超越我们当前仪器的新功能。其中包括用于金属蛋白 EPR 研究的 1.8 K 温度能力和用于增强光谱分辨率的 Q 波段频率，以及通过多频率方法研究天然非结构蛋白和膜蛋白的蛋白质动力学。该仪器还将成为国家高磁场实验室 (NHMFL) 外部用户计划的一部分，并通过该计划向全世界提供请求。此外，这款新型光谱仪将减轻目前在我们的单脉冲 EPR 光谱仪上进行的 CW EPR 实验的负担。佛罗里达大学的十多名教员以及外部合作人员以及中佛罗里达大学的两名教员将受益于此次升级所增加的 EPR 时间和功能。有几个学院属于化学系。许多人是医学院的用户或合作者；有些是新教师。 NIH 资助的需要 EPR 实验的生物医学系统研究因我们的用户群而异，包括纳米粒子、自旋捕获和剂量测量以及放射治疗和氧化损伤应用的研究；参与能量代谢的金属蛋白和无机复合物的表征；对调节细胞周期并参与肿瘤学的天然非结构蛋白进行定点自旋标记（SDSL）研究； SDSL 研究参与脂质和营养物质运输的膜蛋白，以及 SDSL 研究 HIV-1 蛋白酶的天然多态性和耐药结构中的构象采样。

项目成果

Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).

• DOI: 10.1002/0471140864.ps1717s74
• 发表时间: 2013-11-05
• 期刊: Current protocols in protein science
• 作者: de Vera, Ian Mitchelle S;Blackburn, Mandy E;Galiano, Luis;Fanucci, Gail E
• 通讯作者: Fanucci, Gail E

Continuous wave W- and D-band EPR spectroscopy offer "sweet-spots" for characterizing conformational changes and dynamics in intrinsically disordered proteins.

• DOI: 10.1016/j.bbrc.2014.06.045
• 发表时间: 2014-07-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Casey, Thomas M.;Liu, Zhanglong;Esquiaqui, Jackie M.;Pirman, Natasha L.;Milshteyn, Eugene;Fanucci, Gail E.
• 通讯作者: Fanucci, Gail E.

Correlating conformational shift induction with altered inhibitor potency in a multidrug resistant HIV-1 protease variant.

• DOI: 10.1021/bi301010z
• 发表时间: 2012-10-09
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: de Vera IM;Blackburn ME;Fanucci GE
• 通讯作者: Fanucci GE

Ion-dependent mobility effects of the Fusobacterium nucleatum glycine riboswitch aptamer II via site-directed spin-labeling (SDSL) electron paramagnetic resonance (EPR)

• DOI: 10.1016/j.bbrc.2019.06.105
• 发表时间: 2019-08-27
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ehrenberger, Michelle A.;Vieyra, Aleida;Fanucci, Gail E.
• 通讯作者: Fanucci, Gail E.