Portable Brian Injury Biomarker Detection System with Integrated Microdialysis Pr

具有集成微透析功能的便携式布莱恩损伤生物标志物检测系统

• 批准号: 8200201
• 负责人: Champak Das
• 金额: $ 16.58万
• 依托单位: SFC FLUIDICS, LLC
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8200201
• 关键词: Accident and Emergency department; Biological Assay; Biological Markers; Biosensor; Blood capillaries; Brain Injuries; Case Study; Detection; Development; Devices; Diagnosis; Enzyme-Linked Immunosorbent Assay; Glutamates; Healed; Health; In Vitro; Injury; Intervention; Liquid substance; Literature; Magnetism; Measures; Methods; Microdialysis; Microfluidics; Monitor; Nature; Nitric Oxide; Optics; Patients; Performance; Phase; Positioning Attribute; Proteins; Pump; Pyruvate; Quality of Care; Reagent; Recovery; Reporting; Research; Sampling; Sensitivity and Specificity; Site; Small Business Innovation Research Grant; Syringes; System; Systems Analysis; TBI Patients; Technology; Testing; Time; Traumatic Brain Injury; United States; Visit; abstracting; base; capillary; clinically relevant; design; detector; engineering design; healing; improved; instrument; novel; prototype; sensor; small molecule; wasting

DESCRIPTION (provided by applicant): Portable brain injury biomarker detection system with integrated microdialysis probe Project summary/Abstract: This SBIR application aims to integrate microdialysis with an effective, miniature pumping system and a detection system for analyzing different biomarkers in real-time and without user intervention. An integrated portable device consisting of SFC Fluidics' proprietary ePump(R), snap-on magnetic latches for rapid fluidic connection, a reagent reservoir and a microfluidic detection system will be developed. This system will facilitate real time continuous monitoring of pathological biomarkers, which in turn helps in better patient management over the course of healing/therapy. The focus of this SBIR is on monitoring Traumatic Brain Injury (TBI) sequelae with a microdialysis based system using a set of low molar mass biomarkers and a leading large molecule (protein) biomarker. In Phase I, commercially-available ELISA kits will be incorporated into SFC Fluidics integrated microdialysis and fluid handling system. The Phase II focus will be on development of a single prototype system that can quantify multiple TBI biomarkers. Phase I Research Plan Specific Aim: Design and engineer brain injury biomarker detection system with integrated microdialysis probe Task 1: Design and fabricate ePump driven integrated flow system Task 2: Detection of small molecule brain injury biomarkers Task 3: Detection of large molecule brain injury biomarker PUBLIC HEALTH RELEVANCE: An estimated 1.7 million Traumatic Brain injury (TBI) cases are reported every year in United States with 80.7 percent of them resulting in emergency department visits. There is an urgent need for development of a portable, microdialysis system with in-built analyzer which will help in rapid and continuous diagnosis of the state of health of a patient with TBI. Early and timely diagnosis of the pathological condition will help a doctor to take remedial actions and improve the quality of care of the patient while greatly mitigating long-term complications.

描述（由申请人提供）： 便携式脑损伤生物标志物检测系统与集成微透析探针项目摘要/摘要：该SBIR应用旨在将微透析与有效的微型泵送系统和检测系统集成，用于实时分析不同的生物标志物，无需用户干预。将开发一种集成的便携式设备，包括SFC Fluidics专有的ePump（R）、用于快速流体连接的卡扣式磁性闩锁、试剂储存器和微流体检测系统。该系统将促进病理生物标志物的真实的时间连续监测，这反过来有助于在愈合/治疗过程中更好地管理患者。该SBIR的重点是使用一组低摩尔质量生物标志物和一种领先的大分子（蛋白质）生物标志物，通过基于微透析的系统监测创伤性脑损伤（TBI）后遗症。在第一阶段，市售的ELISA试剂盒将被纳入SFC Fluidics集成微透析和流体处理系统。第二阶段的重点将是开发一个单一的原型系统，可以量化多种TBI生物标志物。I期研究计划具体目标：设计和工程化集成微透析探针的脑损伤生物标志物检测系统任务1：设计和制造ePump驱动的集成流动系统任务2：检测小分子脑损伤生物标志物任务3：检测大分子脑损伤生物标志物 公共卫生关系： 据估计，美国每年报告170万例创伤性脑损伤（TBI）病例，其中80.7%导致急诊室就诊。迫切需要开发一种具有内置分析仪的便携式微透析系统，该系统将有助于快速和连续地诊断TBI患者的健康状况。早期和及时诊断病理状况将有助于医生采取补救措施，提高患者的护理质量，同时大大减轻长期并发症。