Portable Brian Injury Biomarker Detection System with Integrated Microdialysis Pr

描述(由申请人提供)： 集成微透析探头的便携式脑损伤生物标志物检测系统项目摘要/摘要：这项SBIR应用旨在将微透析与有效的微型泵送系统和检测系统相结合，以实时分析不同的生物标志物，而无需用户干预。SFC Fluidics将开发一种集成的便携式设备，包括SFC Fluidics专有的ePump(R)、用于快速流体连接的卡扣式磁闩锁、试剂储存库和微流体检测系统。该系统将促进对病理生物标志物的实时连续监测，这反过来又有助于在康复/治疗过程中更好地管理患者。这项SBIR的重点是使用一套低摩尔质量生物标记物和领先的大分子(蛋白质)生物标记物，使用基于微透析的系统监测创伤性脑损伤(TBI)后遗症。在第一阶段，SFC Fluidics集成的微透析和液体处理系统将纳入市面上可买到的试剂盒。第二阶段的重点将是开发一个单一的原型系统，可以量化多个TBI生物标记物。第一阶段研究计划具体目标：设计和设计集成微透析探头的脑损伤生物标记物检测系统任务1：设计和制造ePump驱动的集成流动系统任务2：检测小分子脑损伤生物标记物任务3：检测大分子脑损伤生物标记物 公共卫生相关性： 据估计，美国每年有170万例创伤性脑损伤(TBI)病例，其中80.7%需要急诊科就诊。迫切需要开发一种便携式的、具有内置分析仪的微透析系统，该系统将有助于快速和连续地诊断脑外伤患者的健康状态。早期和及时地诊断这种病理状况将有助于医生采取补救措施，提高患者的护理质量，同时极大地减轻长期并发症。

DESCRIPTION (provided by applicant): Portable brain injury biomarker detection system with integrated microdialysis probe Project summary/Abstract: This SBIR application aims to integrate microdialysis with an effective, miniature pumping system and a detection system for analyzing different biomarkers in real-time and without user intervention. An integrated portable device consisting of SFC Fluidics' proprietary ePump(R), snap-on magnetic latches for rapid fluidic connection, a reagent reservoir and a microfluidic detection system will be developed. This system will facilitate real time continuous monitoring of pathological biomarkers, which in turn helps in better patient management over the course of healing/therapy. The focus of this SBIR is on monitoring Traumatic Brain Injury (TBI) sequelae with a microdialysis based system using a set of low molar mass biomarkers and a leading large molecule (protein) biomarker. In Phase I, commercially-available ELISA kits will be incorporated into SFC Fluidics integrated microdialysis and fluid handling system. The Phase II focus will be on development of a single prototype system that can quantify multiple TBI biomarkers. Phase I Research Plan Specific Aim: Design and engineer brain injury biomarker detection system with integrated microdialysis probe Task 1: Design and fabricate ePump driven integrated flow system Task 2: Detection of small molecule brain injury biomarkers Task 3: Detection of large molecule brain injury biomarker PUBLIC HEALTH RELEVANCE: An estimated 1.7 million Traumatic Brain injury (TBI) cases are reported every year in United States with 80.7 percent of them resulting in emergency department visits. There is an urgent need for development of a portable, microdialysis system with in-built analyzer which will help in rapid and continuous diagnosis of the state of health of a patient with TBI. Early and timely diagnosis of the pathological condition will help a doctor to take remedial actions and improve the quality of care of the patient while greatly mitigating long-term complications.