Mouse Indirect Calorimetry and Tracer System

描述（由申请方提供）：科罗拉多临床营养研究单位的能量平衡核心实验室需要来自哥伦布仪器（哥伦布，OH）的新型小鼠综合代谢监测系统（CLAMS-8 M）。间接量热系统用于测量氧消耗和二氧化碳产生，由此可以确定能量代谢的许多方面，包括代谢率和呼吸商。目前的4笼系统已经过时，最初建于1996年，并大量使用。目前系统中的计算机系统和接口板只能在视窗98上操作，与较新的微软操作系统和计算机不兼容。新的间接热量计不仅将使我们的容量增加一倍，达到8个笼子，而且还将增加其他功能，例如实时测量食物和水的消耗量沿着运动评估，所有这些都是我们目前的系统无法跟踪的。具有更大的容量也将减少实验间误差。此外，该系统还将被修改为与放射性示踪剂一起使用，以测量组织隔室和二氧化碳呼气中燃料的运输。新的间接量热系统的笼子将被封闭在一个环境室中，以便精确控制照明，温度和湿度，并允许研究人员在不同的环境温度下进行实验，包括：1）热中性条件（环境温度，在该环境温度下，动物消耗最少的能量来维持体温）和2）冷-暴露以进一步干扰表型并检测在标准环境温度下可能不明显的差异。新的间接量热系统将根据批准的动物卫星设施的规定进行操作，并将为CNRU核心设施的用户提供大量资源，提供最先进的设备，以满足日益增长的对小鼠能量代谢和燃料利用的更全面、可靠测量的需求。 公共卫生相关性：科罗拉多临床营养研究单位的能量平衡核心实验室需要一种新的设备，用于测量小鼠的新陈代谢。 有了这些信息，我们也许可以更好地了解未来的治疗和预防人类肥胖。

DESCRIPTION (provided by applicant): The Colorado Clinical Nutrition Research Unit's Energy Balance Core Laboratory is in need of a new comprehensive metabolic monitoring system for mice (CLAMS-8M) from Columbus Instruments (Columbus, OH). An indirect calorimetry system is used to measure oxygen consumption and carbon dioxide production from which many aspects of energy metabolism can be determined including metabolic rate and respiratory quotient. The current 4 cage system is outdated, was originally built in 1996 and is heavily used. The computer system and interface boards in the current system only operate on Windows 98 and are not compatible with newer Microsoft operating systems and computers. The new indirect calorimeter will not only double our capacity to 8 cages, but will add other features such as real-time measurements of food and water consumption along with assessments of exercise, all of which our current system is unable to track. Having a larger capacity will also reduce the inter-experimental error. Additionally, the system will also be modified to be used with radioactive tracers to measure trafficking of fuels in tissue compartments and in carbon dioxide expiration. The caging for the new indirect calorimetry system will be enclosed in an environmental chamber so that lighting, temperature and humidity can be controlled precisely and allow investigators to conduct experiments at different environmental temperatures including: 1) thermoneutrality conditions (ambient temperature at which animals expends the least amount of energy to maintain body temperature) and 2) cold-exposure to further perturb the phenotype and detect differences which may not have been noticeable at standard ambient temperatures. The new indirect calorimetry system will be operated in accordance to regulations of an approved Animal Satellite Facility and will provide a great resource for users of the CNRU's core facilities, providing state of the art equipment for the increasing need for more comprehensive, reliable measurements of energy metabolism and fuel utilization in mice. PUBLIC HEALTH RELEVANCE: The Colorado Clinical Nutrition Research Unit's Energy Balance Core Laboratory is in need of a new piece of equipment that will be used to measure the metabolism of mice. With the information, we can perhaps achieve a better understanding of future treatments and prevention of obesity in humans.