BD FACSAria II Flow Cytometer

描述(由申请者提供)：Gladstone Flow Core正在申请资金购买一台Becton Dickinson FACSAria II，专门为Gladstone研究所的研究而定制。该细胞分选机配备了可选的黄绿色、紫外光和高功率紫色激光器、自动细胞沉积单元和温度控制。这些选项中的每一个都经过了仔细的考虑，并满足了NIH资助的七名Gladstone调查人员的九项研究中的一项的需要。这些项目集中在与心血管和传染病有关的广泛基础研究上。Gladstone流动岩芯主管Marielle Cavrois将管理该仪器，并由我们训练有素的技术人员监督其操作。选择FACSAria II是因为其固定的光学系统，这一功能简化了校准和维护，并将允许主要用户在经过Gladstone Flow Core员工的培训后自行操作仪器，从而延长操作时间，使我们的主要用户群体受益。FACSAria II拥有气溶胶管理选项，将安装在Gladstone BSL-3实验室，以确保对受血液传播病原体污染的样本进行安全的细胞分类。这一独特的功能与多个激光选项相结合，将对不断发展的观澜湾园区的更广泛的研究社区具有极高的价值，可以向他们提供未分配给我们主要用户组的剩余时间。FACSAria II流式细胞仪是Gladstone对现代最重要的三个医学问题：心血管疾病、艾滋病毒/艾滋病和阿尔茨海默病的基本基础研究。

DESCRIPTION (provided by applicant): The Gladstone Flow Core is requesting funds to purchase a Becton Dickinson FACSAria II specifically customized for research at the Gladstone Institutes. This cell sorter is equipped with optional yellow-green, UV, and high-powered violet lasers, an automatic cell deposition unit, and a temperature control. Each of these options was carefully considered and responds to the need of one of the nine NIH-funded studies from seven Gladstone investigators. These projects are centered on a broad range of basic research in relation to cardiovascular and infectious diseases. Marielle Cavrois, the Director of the Gladstone Flow Core, will administer the instrument and oversee its operation by our highly trained technician. The FACSAria II was selected for its fixed optical system, a feature that simplifies calibration and maintenance, and will permit the major users to self-operate the instrument after training by the Gladstone Flow Core staff, extending the hours of operation for the benefit of our major users group. The FACSAria II possesses an aerosol management option and will be sited in the Gladstone BSL-3 laboratory to ensure safe cell sorting of samples contaminated with blood-borne pathogens. This unique feature, coupled with the multiple laser options, will be highly valuable to the wider research community on the growing Mission Bay campus, to whom the remaining time not allocated to our major users group can be made available. The FACSAria II flow cytometer is essential Gladstone's basic research on three of the most important medical problems of modern times: cardiovascular disease, HIV/AIDS and Alzheimer's disease.