BD FACSAria II Flow Cytometer

BD FACSAria II 流式细胞仪

• 批准号: 7840236
• 负责人: MARIELLE CAVROIS
• 金额: $ 54.45万
• 依托单位: J. DAVID GLADSTONE INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7840236
• 关键词: AIDS/HIV problem; Aerosols; Alzheimer' s Disease; Basic Science; Blood-Borne Pathogens; Calibration; Cardiovascular Diseases; Cell Separation; Cells; Communicable Diseases; Communities; Coupled; Deposition; Ensure; Funding; Hour; Institutes; Laboratories; Lasers; Maintenance; Medical; Mission; Optics; Research; Research Personnel; Sampling; Site; System; Temperature; Time; Training; United States National Institutes of Health; Viola; instrument; operation

DESCRIPTION (provided by applicant): The Gladstone Flow Core is requesting funds to purchase a Becton Dickinson FACSAria II specifically customized for research at the Gladstone Institutes. This cell sorter is equipped with optional yellow-green, UV, and high-powered violet lasers, an automatic cell deposition unit, and a temperature control. Each of these options was carefully considered and responds to the need of one of the nine NIH-funded studies from seven Gladstone investigators. These projects are centered on a broad range of basic research in relation to cardiovascular and infectious diseases. Marielle Cavrois, the Director of the Gladstone Flow Core, will administer the instrument and oversee its operation by our highly trained technician. The FACSAria II was selected for its fixed optical system, a feature that simplifies calibration and maintenance, and will permit the major users to self-operate the instrument after training by the Gladstone Flow Core staff, extending the hours of operation for the benefit of our major users group. The FACSAria II possesses an aerosol management option and will be sited in the Gladstone BSL-3 laboratory to ensure safe cell sorting of samples contaminated with blood-borne pathogens. This unique feature, coupled with the multiple laser options, will be highly valuable to the wider research community on the growing Mission Bay campus, to whom the remaining time not allocated to our major users group can be made available. The FACSAria II flow cytometer is essential Gladstone's basic research on three of the most important medical problems of modern times: cardiovascular disease, HIV/AIDS and Alzheimer's disease.

描述（由申请人提供）：格莱斯顿流动核心正在申请资金购买专门为格莱斯顿研究所研究定制的Becton Dickinson FACSAria II。该细胞分选仪配备了可选的黄绿色、紫外线和高功率紫色激光器、自动细胞沉积单元和温度控制。这些选择中的每一个都经过了仔细的考虑，并回应了来自七名格莱斯顿研究人员的九项NIH资助研究之一的需求。这些项目集中于与心血管和传染病有关的广泛基础研究。格莱斯顿流动核心的主任Marielle Cavrois将管理仪器并由我们训练有素的技术人员监督其操作。选择FACSAria II是因为它的固定光学系统，这一功能简化了校准和维护，并允许主要用户在接受Gladstone Flow Core工作人员的培训后自行操作仪器，延长了操作时间，使我们的主要用户群体受益。FACSAria II具有气溶胶管理选项，并将位于Gladstone BSL-3实验室，以确保对受血液传播病原体污染的样本进行安全的细胞分选。这一独特的功能，加上多种激光器的选择，将是非常有价值的更广泛的研究界在不断增长的使命湾校园，对他们的剩余时间没有分配给我们的主要用户组可以提供。FACSAria II流式细胞仪是Gladstone对现代三个最重要的医学问题进行基础研究的基础：心血管疾病，艾滋病毒/艾滋病和阿尔茨海默病。