Purchase of a Liquid Chromatograph-Orbitrap VELOS mass spectrometer

购买液相色谱-Orbitrap VELOS 质谱仪

• 批准号: 8052978
• 负责人: Sonja Hess
• 金额: $ 60万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052978
• 关键词: Alzheimer' s Disease; Area; Autoimmune Diseases; Bioinformatics; Biological; California; Cells; Communities; Coupled; Disease; Dissociation; Electron Transport; Equipment; Funding; Generations; Genetic; Goals; Grant; Infection; Institutes; Ions; Laboratories; Ligands; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Methods; Monitor; Parkinson Disease; Pharmaceutical Preparations; Play; Postdoctoral Fellow; Price; Proteins; Proteome; Proteomics; Research; Resources; Role; Sampling; Scanning; Source; Students; System; Techniques; Technology; United States National Institutes of Health; Vascular Diseases; Work; base; insight; instrument; mass spectrometer; nervous system disorder; novel; prevent

DESCRIPTION (provided by applicant): We request funding for a new Orbitrap Velos ETD (Thermo) and plan to integrate this in the Proteome Exploration Laboratory (PEL) of the Beckman Institute at the California Institute of Technology (Caltech). The PEL was established in 2007 and is the only resource center at Caltech where mass spectrometry-based proteome analyses can be performed. The PEL collaborates with students and postdoctoral fellows of the Caltech community to enable their proteomics-related research. The main long-term objective of the PEL is to enable the in-depth elucidation and quantification of proteomes and subproteomes to gain fundamental insights into biological regulatory mechanisms. A common experimental paradigm is to monitor global protein changes that occur after either treating cells with drugs or natural ligands or subjecting them to an environmental or genetic perturbation. Novel methods are constantly being developed to achieve this goal. We request funding for two main reasons. First, demand in the Caltech community has overwhelmed our current suite of instruments. Second and more importantly, technological limitations of our current instruments are hampering the progress of NIH-funded projects in the laboratories of our collaborators. The Orbitrap Velos ETD has the ability to transmit ions with higher efficiency and scan faster than the first generation instruments, thus increasing overall sensitivity. In addition, higher energy C-trap dissociation (HCD) and electron transfer dissociation (ETD) offer complementary fragmentation techniques not available to us, but essential for the progress of the proposed projects. Moreover, the Proxeon Easy nanoHPLC with nanoESI source that we acquired in anticipation of purchasing an Orbitrap Velos allows for rapid switching between 1D and 2D LC separations, which is critical given the great range in complexity of samples that are analyzed at the PEL. To demonstrate our commitment to this application, the PEL has already purchased essential auxiliary equipment for the Orbitrap Velos and will use money obtained from private sources to cover the difference between the maximum allowable funding under this grant mechanism and the purchase price of the Orbitrap Velos. An Orbitrap Velos mass spectrometer coupled with the Proxeon LC system and source, working in concert with our existing bioinformatics pipeline, will play a vital role in advancing projects that span a broad range of fundamental and applied studies that include cancer, neurological disorders (such as Alzheimer's disease, Parkinson), autoimmune diseases, vascular diseases and infections. Fundamental insights in any of these areas will help to prevent and/or cure diseases that have previously been considered incurable.

描述（由申请人提供）：我们要求为新的Orbitrap Velos ETD（Thermo）提供资金，并计划将其集成到加利福尼亚理工学院贝克曼研究所（CALTECH）的贝克曼研究所的Proteome Exploration Laboratory（PEL）中。 PEL成立于2007年，是加州理工学院唯一可以进行基于质谱的蛋白质组分析的资源中心。 PEL与Caltech社区的学生和博士后研究员合作，以实现与蛋白质组学相关的研究。 PEL的主要长期目标是实现蛋白质组和亚蛋白质体的深入阐明和定量，以获得对生物调节机制的基本见解。一个常见的实验范式是监测用药物或天然配体处理细胞或使其经历环境或遗传扰动后发生的全局蛋白质变化。不断开发新的方法来实现这一目标。我们要求提供资金，原因有两个。首先，加州理工学院社区的需求使我们目前的工具套件不堪重负。其次，更重要的是，我们当前工具的技术限制正在阻碍合作者实验室中NIH资助的项目的进步。与第一代仪器相比，Orbitrap Velos ETD具有更高效率和扫描更快的传输离子的能力，从而提高了整体敏感性。此外，较高的能量C陷阱解离（HCD）和电子转移解离（ETD）提供了互补的碎片化技术，但对于拟议项目的进展至关重要。此外，我们在购买Orbitrap Velos时获得的带有纳米源的Proxeon Easy Nanohplc允许在1D和2D LC分离之间快速切换，这一点至关重要，鉴于在PEL上分析的样品的复杂性范围很大。为了证明我们对该应用程序的承诺，PEL已经为Orbitrap Velos购买了必需的辅助设备，并将使用从私人资源获得的资金来涵盖此赠款机制下的最大允许资金与Orbitrap Velos的购买价格之间的差额。 Orbitrap Velos质谱仪与Proxeon LC系统和来源一起与我们现有的生物信息学管道合作，将在跨越包括癌症，神经系统疾病的广泛基础和应用研究的项目中发挥至关重要的作用，例如，神经系统疾病（例如，阿尔茨海默氏病，帕克森氏症），自动疾病，自动疾病。这些领域中任何一个的基本见解都将有助于预防和/或治愈以前被认为无法治愈的疾病。

项目成果

O-linked glycosylation sites profiling in Mycobacterium tuberculosis culture filtrate proteins.

• DOI: 10.1016/j.jprot.2013.05.011
• 发表时间: 2014-01-31
• 期刊: Journal of proteomics
• 影响因子: 3.3
• 作者: Smith GT;Sweredoski MJ;Hess S
• 通讯作者: Hess S

ETD Outperforms CID and HCD in the Analysis of the Ubiquitylated Proteome.

• DOI: 10.1007/s13361-015-1168-0
• 发表时间: 2015-09
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Porras-Yakushi TR;Sweredoski MJ;Hess S
• 通讯作者: Hess S

Evaluation and optimization of mass spectrometric settings during data-dependent acquisition mode: focus on LTQ-Orbitrap mass analyzers.

• DOI: 10.1021/pr3011588
• 发表时间: 2013-07-05
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Kalli A;Smith GT;Sweredoski MJ;Hess S
• 通讯作者: Hess S

Vectored antibody gene delivery mediates long-term contraception.

载体抗体基因递送介导长期避孕。

• DOI: 10.1016/j.cub.2015.08.002
• 发表时间: 2015
• 期刊: Current biology : CB
• 作者: Li,Juan;Olvera,AlejandraI;Akbari,OmarS;Moradian,Annie;Sweredoski,MichaelJ;Hess,Sonja;Hay,BruceA
• 通讯作者: Hay,BruceA