Acquisition of a Q-Exactive Plus mass spectrometer.

购买 Q-Exactive Plus 质谱仪。

• 批准号: 8826551
• 负责人: Sonja Hess
• 金额: $ 60万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-10 至 2017-02-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826551
• 关键词: Area; Bacterial Infections; Bioinformatics; Biological; California; Cells; Communities; Coupled; Development; Developmental Process; Disease; Funding; Genetic; Individual; Institutes; Ions; Laboratories; Ligands; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Methods; Monitor; Pharmaceutical Preparations; Phase; Play; Postdoctoral Fellow; Process; Proteins; Proteome; Proteomics; Research; Research Personnel; Resources; Role; Sampling; Students; System; Technology; United States National Institutes of Health; Work; base; insight; instrument; instrumentation; mass spectrometer; meetings; nano; prevent; success; technological innovation

 DESCRIPTION (provided by applicant): The aim of this proposal is to request funding for a new state-of-the-art nano UPLC Q-Exactive Plus mass spectrometer from Thermo Scientific, which we plan to integrate into the Proteome Exploration Laboratory (PEL) of the Beckman Institute at the California Institute of Technology (Caltech) to replace our existing Orbitrap Classic mass spectrometer, which is now reaching the maximum expected lifetime and thus, Thermo is phasing out their support on this instrument. We request funding because technological limitations of our current instruments are no longer meeting methodological demands of the increasing number of users, hampering the progress of NIH-funded projects in the laboratories of our collaborators. Due to technological innovations, the Orbitrap Classic can effectively no longer compete with current state-of-the-art technology as a result of the tremendous instrumentation developments during the past years; and is therefore hindering our advancement in the field of proteomics. The Q-Exactive Plus also offers unique measurement capabilities due to its quadrupole filter rather than an ion trap, which will enable many projects t the PEL that we can currently not carry out. The PEL was established in 2007 and is the only resource center at Caltech where mass spectrometry-based proteomic analyses can be performed. The PEL was initially established to primarily collaborate with students and postdoctoral fellows of the Caltech community to develop new methods and advance their proteomics-related research. Due to its commitment to excellence, the PEL is frequently able to analyze difficult samples that typical facilities may not be successful with. As a consequence, the PEL has developed into a center that is also sought out by other researchers as well, local and state-wide. The overwhelming success of our proteomics setup has steadily increased the demand at the PEL and continues to increase further. The main long-term objective of the PEL is to enable the in-depth elucidation and quantification of proteomes, subproteomes and individual proteins to gain fundamental insights into biological regulatory processes. A common experimental paradigm is to monitor global protein changes that occur after either treating cells with drugs or natural ligands; or subjecting them to an environmental or genetic perturbation. A Q-Exactive Plus mass spectrometer coupled with the EASY nano UPLC system, working in concert with our existing bioinformatics pipeline, will play a vital role in advancing projects tha span a broad range of fundamental (developmental processes) and applied studies (cancer, bacterial infections). Fundamental insights into any of these areas will help prevent and/or cure diseases that have previously been considered incurable.

 描述（申请人提供）：该提案的目的是为Thermo Scientific的新型最先进的纳米UPLC Q-Exactive Plus质谱仪申请资金，我们计划将其整合到加州理工学院（Caltech）贝克曼研究所的蛋白质组探索实验室（PEL）中，以取代我们现有的Orbitrap Classic质谱仪，现在达到了最大预期寿命，因此，Thermo正在逐步停止对该仪器的支持。我们要求资金，因为我们目前的仪器的技术限制不再满足越来越多的用户的方法学需求，阻碍了NIH资助的项目在我们合作者的实验室的进展。由于技术创新，Orbitrap Classic在过去几年中由于仪器的巨大发展而不再能够有效地与当前最先进的技术竞争;因此阻碍了我们在蛋白质组学领域的进步。Q-Exactive Plus还提供了独特的测量功能，因为它的四极滤波器而不是离子阱，这将使我们目前无法执行的许多项目能够在PEL上进行。PEL成立于2007年，是加州理工学院唯一的资源中心，可以进行基于质谱的蛋白质组学分析。PEL最初成立的主要目的是与加州理工学院社区的学生和博士后研究员合作，开发新方法并推进他们的蛋白质组学相关研究。由于其对卓越的承诺，PEL经常能够分析典型设施可能无法成功的困难样品。因此，PEL已经发展成为一个中心，也是由其他研究人员以及，地方和国家范围内寻求。我们蛋白质组学设置的巨大成功稳步增加了PEL的需求，并将继续进一步增加。PEL的主要长期目标是能够深入阐明和定量蛋白质组，亚蛋白质组和单个蛋白质，以获得对生物调控过程的基本见解。一种常见的实验范式是监测用药物或天然配体处理细胞后发生的整体蛋白质变化;或使它们受到环境或遗传干扰。Q-Exactive Plus质谱仪与EASY nano UPLC系统相结合，与我们现有的生物信息学管道协同工作，将在推进涵盖广泛的基础（开发过程）和应用研究（癌症，细菌感染）的项目中发挥至关重要的作用。对这些领域的基本见解将有助于预防和/或治愈以前被认为无法治愈的疾病。

项目成果

Interrogating marine virus-host interactions and elemental transfer with BONCAT and nanoSIMS-based methods.

使用 BONCAT 和基于 nanoSIMS 的方法研究海洋病毒与宿主的相互作用和元素转移。

• DOI: 10.1111/1462-2920.13996
• 发表时间: 2018
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Pasulka,AlexisL;Thamatrakoln,Kimberlee;Kopf,SebastianH;Guan,Yunbin;Poulos,Bonnie;Moradian,Annie;Sweredoski,MichaelJ;Hess,Sonja;Sullivan,MathewB;Bidle,KayD;Orphan,VictoriaJ
• 通讯作者: Orphan,VictoriaJ