Acquisition of a Leica TCS SP5 Laser Scanning Confocal Microscope

描述(申请人提供)：激光扫描共聚焦显微镜(LSCM)已成为生物组织中荧光标记结构和分子的高分辨率三维光学成像的有力工具。在过去的十年里，亚利桑那州立大学通过创建新的跨学科分子和细胞生物学和神经科学课程，并通过招聘新的教职员工，在NIH资助的研究中实施光学成像，有力地扩展了分子生命科学。LSCM目前由亚利桑那州立大学于1997年创建的核心多用户W.M.Keck生物成像实验室提供。我们开发了这项由几名NIH资助的研究人员组成的设备赠款，以确保购买徕卡TCS SP5 LSCM的资金，以保持亚利桑那州立大学强大的LSCM能力。收购这一工具将使这项提案中的调查人员在继续获得联邦资金方面具有竞争力，并促进正在进行申请和审查过程的初级调查人员的成长。此外，该仪器将允许神经科学家、分子生物学家、发育生物学家和生物工程师继续依靠体内和体外三维定量成像进行尖端研究。该仪器具有高度的通用性，将促进顶级研究应用，从研究昆虫大脑的记忆痕迹，到脊髓运动神经元的结构变化，参与脊柱侧弯的分子网络，再到研究RNA病毒和蛋白质-蛋白质相互作用。亚利桑那州立大学坚定地致力于维护和进一步发展核心的W.M.Keck生物成像实验室，所要求的仪器将极大地增强亚利桑那州立大学日益壮大的NIH资助的生物医学研究人员的研究能力。 公共卫生相关性：需要资金购买徕卡TCS SP5激光扫描共聚焦显微镜，作为放置在亚利桑那州立大学W.M.Keck生物成像实验室核心的多用户设备。该仪器将允许在活的和组织准备的生物组织中的荧光标记结构和分子的三维空间维度进行高分辨率光学成像。因此，该系统将增强亚利桑那州立大学由NIH资助的一大批生物医学研究人员的研究能力。

DESCRIPTION (provided by applicant): Laser scanning confocal microscopy (LSCM) has become a powerful tool for high resolution three dimensional optical imaging of fluorescence labeled structures and molecules in biological tissue. Over the last ten years Arizona State University has strongly expanded in the molecular life sciences by creating new interdisciplinary molecular and cellular biology and neuroscience programs, and by hiring new faculty members, who implement optical imaging into their NIH funded research. LSCM is currently offered at the core multi-user W.M. Keck Bioimaging Laboratory founded by ASU in 1997. We developed this equipment grant composed of several NIH funded investigators to secure funds to purchase a Leica TCS SP5 LSCM for maintaining strong 'state of the art' LSCM capabilities at ASU. The acquisition of this instrument will permit investigators in this proposal to be competitive in continuing to secure federal funding, as well as foster the growth of junior investigators, who are undergoing the application and review process. Further, the instrument will permit neuroscientists, molecular biologists, developmental biologists and bioengineers to continue cutting-edge research that relies on quantitative imaging in three dimensions in vivo and in vitro. The instrument is highly versatile and will foster top research applications ranging from the study of memory traces in insect brains, to structural changes in spinal motoneurons, molecular networks involved in Scoliosis to studying RNA viruses and protein-protein interactions. ASU shows strong commitment to maintain and further develop the core W.M. Keck Bioimaging Laboratory, and the requested instrument will greatly augment the research capabilities of the growing group of NIH-funded biomedical researchers at ASU. PUBLIC HEALTH RELEVANCE: Requested are funds to purchase a Leica TCS SP5 laser scanning confocal microscope as a multi-user piece of equipment to be placed in the core W.M. Keck Bioimaging Laboratory at Arizona State University. This instrument will allow high resolution optical imaging in three spatial dimensions of fluorescence labeled structures and molecules in living and histologically prepared biological tissue. The system will therefore augment the research capabilities of a large group of NIH-funded biomedical researchers at ASU.