Transmission Electron Microscope

透射电子显微镜

• 批准号: 8052990
• 负责人: Patricia Ann D'Amore
• 金额: $ 43.39万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052990
• 关键词: Accounting; Advisory Committees; Cells; Computers; Contract Services; Contracts; Data; Electron Microscope; Electrons; Elements; Equipment; Eye; Fee-for-Service Plans; Fees and Charges; Funding; Funding Applicant; Guns; Head; Histology; Image; Imagery; Institution; Letters; Maintenance; Mediation; Membrane Biology; Microscope; Microtome - medical device; Minor; Morphology; Nanotechnology; Optics; Principal Investigator; Research; Research Institute; Sampling; Services; Staging; Staging System; Staining method; Stains; Three-Dimensional Imaging; Time; Tissues; Vacuum; Work; base; charge coupled device camera; cost; discount; instrument; lens; novel; operation; programs; tissue preparation; tomography; transmission process

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a Tecnai G2 Spirit BioTWIN G2 transmission electron microscope (TEM) and microtome. This TEM is a state-of-the-art instrument and contains a high-contrast BioTWIN objective lens optimized for 2D and 3D imaging of stained or unstained, low-contrast samples. All of the microscope components including the electron gun, the optical elements, the vacuum system and the stage, are digitally controlled, allowing the user to automatically recall all optimized operating conditions including lens settings, gun parameters, optical alignments, aperture alignments. Similarly, the CCD camera is computer controlled so that image acquisition can be automated and connected to a network for exporting data. Four major users and seven minor users, whose work is supported by 16 RO1's, and 3 R21's, 1 PO1 and 1 DP2, will utilize the microscope. These users will account for approximately 32 hr/week (80% usage of the microscope time). The remainder of the time will be available for new users at Schepens Eye Research Institute (SERI) and, when available, for outside users. The requested TEM will replace the current a Phillips 41OLS, which is 25 years old and for which replacement parts are no longer available. The Tecnai G2 Spirit BioTWIN G2 lists at $659,492 but we have negotiated a very significant discount and have a quote to purchase the scope, camera and tomography stage at $600,000. The high internal usage of the scope makes it infeasible to use services at neighboring institutions. The scope will be integrated into the Morphology Unit and will be overseen and operated by Ms. Patricia Pearson, who has been an integral part of our Morphology Unit for over 30 years and is recognized as an expert in the field of ocular histology. A fulltime research technician, supported by SERI, who will be responsible for tissue preparation and sectioning, will assist Ms. Pearson. Work on the TEM will be carried out as a fee-for-service, with work done on a first come-first serve basis. The service contract will be with Nanotech, who specializes in the service of Philips and FEI electron microscopes, and who has been servicing our current scope for more than 20 years. The cost of the contract is built into the fees that are charged for the TEM services. SERI also maintains a fund for the service and maintenance of common equipment and these funds will be available for expenses not covered in any contract, as was done with the Phillips 41OLS. An internal advisory committee will assist the Principal Investigator with maximizing the utilization and overseeing the day-to-day management of the microscope as well as with establishing a financial plan for the long-term operation and maintenance of the microscope. Dr. Dennis Brown who heads the Imaging Core for the Mass General Program in Membrane Biology has been added to the advisory committee and will be available for advice on novel applications such as tomography. Institutional commitment is evidenced in the included letter from Dr. Eli Peli, a Co- Director of Research at SERI. As more and more of our research has come to rely on ultrastructural visualization of cells and tissues, demand for the TEM has grown and access to this state of the art TEM will undoubtedly facilitate and support our work. ) PUBLIC HEALTH RELEVANCE: Transmission electron microscopy (TEM) permits the visualization of cells and tissues at the subcellular level by providing very high magnification. There has been an increasing utilization of imaging technologies to understand the structure/function of genes of interest. The requested TEM will be used to examine how certain genes and proteins are involved in normal visual processes and how they are altered in diseased tissues of the eye.

描述（由申请人提供）：申请资金用于购买Tecnai G2 Spirit BioTWIN G2透射电子显微镜（TEM）和显微切片机。该TEM是一种最先进的仪器，包含一个高对比度的BioTWIN物镜，用于染色或未染色的低对比度样品的2D和3D成像。所有的显微镜组件，包括电子枪、光学元件、真空系统和工作台，都是数字控制的，允许用户自动回忆所有优化的操作条件，包括镜头设置、枪参数、光学校准、孔径校准。同样，CCD摄像机是由计算机控制的，因此图像采集可以自动化，并连接到网络以导出数据。4个主要用户和7个次要用户将使用显微镜，他们的工作由16个RO1, 3个R21, 1个PO1和1个DP2支持。这些用户将占约32小时/周（80%的显微镜使用时间）。剩下的时间将提供给Schepens眼科研究所（SERI）的新用户，如果可以的话，也提供给外部用户。所要求的TEM将替换现有的菲利普斯41OLS，这是25年的历史，更换零件不再可用。Tecnai G2 Spirit BioTWIN G2的标价为659,492美元，但我们已经协商了一个非常大的折扣，并以600,000美元的价格报价购买了瞄准镜、相机和断层扫描台。范围的高内部使用率使得在邻近机构使用服务变得不可行。该范围将被整合到形态学单元，由Patricia Pearson女士监督和操作，她在我们的形态学单元工作了30多年，是公认的眼部组织学领域的专家。由SERI支持的全职研究技术人员将负责组织准备和切片，协助Pearson女士。TEM的工作将以付费服务的方式进行，工作将以先到先得的方式完成。服务合同将与Nanotech公司签订，Nanotech公司专门为飞利浦和FEI电子显微镜提供服务，并且已经为我们目前的范围提供服务超过20年。合同的成本包含在TEM服务的费用中。SERI还为通用设备的服务和维护保留了一笔资金，这些资金将用于任何合同中未包括的费用，就像Phillips 41OLS一样。一个内部咨询委员会将协助首席研究员最大限度地利用和监督显微镜的日常管理，并为显微镜的长期运行和维护制定财务计划。负责膜生物学大众综合项目成像核心的丹尼斯·布朗博士已加入咨询委员会，并将在断层扫描等新应用方面提供建议。机构承诺是由Eli Peli博士，在SERI研究的联合主任所附的信证明。随着我们越来越多的研究依赖于细胞和组织的超微结构可视化，对透射电镜的需求也在增长，获得这种最先进的透射电镜无疑将促进和支持我们的工作。