Investigation of endomucin as a novel regulator of angiogenesis

内粘蛋白作为血管生成的新型调节剂的研究

• 批准号: 9414681
• 负责人: Patricia Ann D'Amore
• 金额: $ 49.25万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9414681
• 关键词: Address; Adult; Age related macular degeneration; Binding; Biological Assay; Birth; Blindness; Blood Vessels; Blood capillaries; Cell Communication; Cell physiology; Cell surface; Cells; Choroidal Neovascularization; Cytoplasmic Tail; Development; Diabetic Retinopathy; Disease; Endothelial Cells; Equilibrium; Event; Extracellular Domain; Eye; Glycoproteins; Growth; Growth Factor; Histology; Human; ICAM2 gene; Impairment; In Vitro; Investigation; KDR gene; Lasers; Lead; Legal Blindness; Life; Light; Macular degeneration; Measures; Mediating; Messenger RNA; Modeling; Molecular; Mucins; Mus; Mutation; Ocular Pathology; Oxygen; Pathologic; Pathologic Neovascularization; Pathology; Permeability; Persons; Phosphoric Monoester Hydrolases; Phosphorylation; Play; Process; Proteins; Regulation; Reporting; Reproduction; Retinal; Retinal Diseases; Retinal Neovascularization; Retinopathy of Prematurity; Role; Signal Transduction; Small Interfering RNA; Subfamily lentivirinae; Surface; System; Testing; Therapeutic; Tube; Tyrosine Phosphorylation Site; Vascular Endothelial Growth Factor Receptor-1; Vascular Endothelial Growth Factors; Vascular Permeabilities; Vascularization; Veins; Wound Healing; angiogenesis; antiangiogenesis therapy; crosslink; density; design; endothelial-specific sialomucin; in vivo; inhibitor/antagonist; intravitreal injection; knock-down; loss of function; migration; mutant; new growth; novel; novel therapeutics; ocular neovascularization; overexpression; postnatal; proliferative diabetic retinopathy; promoter; retina blood vessel structure; retinal angiogenesis; small hairpin RNA

ABSTRACT Angiogenesis, the growth of new blood vessels, is an important component of development, wound healing, and reproduction as well as a number of pathologies. Abnormal retinal angiogenesis leads to vision loss in a range of ocular pathologies including diabetic retinopathy, wet macular degeneration, and the retinopathy of prematurity. Endomucin (EMCN) is a mucin-like glycoprotein specifically expressed by endothelial cells of veins and capillaries. Our preliminary studies reveal a role for EMCN in the regulation of VEGF-induced endothelial functions in vitro and in normal angiogenesis in vivo. Reduction of EMCN expression using siRNA led to decreased VEGF-stimulated endothelial proliferation, migration and tube formation in vitro, and impaired retinal vascularization in vivo. This application proposes to test the hypothesis that EMCN plays a role in VEGF-induced pathologic neovascularization and that EMCN influences VEGF-stimulated functions by modulating its signaling. Studies are outlined to examine the role of EMCN in pathological angiogenesis using models of abnormal retinal and choroidal blood vessel growth as well as to understand the molecular mechanism that underlies EMCN's effects on VEGF-induced endothelial cell functions using in vitro systems. Studies in Aim 1 will examine the role of EMCN in the development of pathologic ocular neovascularization in vivo. The oxygen-induced model of retinal neovascularization and the laser-induced model of choroidal neovascularization will be utilized in conjunction with both gain and loss of function studies, using both siRNA and lentivirally-delivered shEMCN under the control of ICAM2, an endothelial-specific promoter, as well as adEMCN under the control of ICAM2. The effect of EMCN knockdown and overexpression on pathologic vessel growth will be quantified. Aim 2 proposes to elucidate the molecular mechanism of EMCN's role in the regulation of VEGF-induced endothelial functions. Studies using EMCN deletion mutants will be employed to determine if the effects of EMCN are mediated at the cell surface. The role of EMCN at the cell surface will be further investigated by examining its effect on VEGF binding and VEGFR2 stabilization/internalization. Intracellular effects will focus on a possible role of EMCN phosphorylation, the effect of EMCN on VEGFR2 phosphatase activity as well as the identification of potential EMCN intracellular binding partners. Results of these proposed studies will provide a better understanding of the role of EMCN in normal and pathologic angiogenesis and may lead to the development of novel endothelial cell-specific therapies for the suppression of vessel growth. !

摘要 血管生成，即新血管的生长，是发育、伤口愈合、 和繁殖以及一些病理现象。视网膜血管生成异常导致视力丧失 一系列眼部病变，包括糖尿病视网膜病变、湿性黄斑变性和 早产儿。血管内粘蛋白(EMCN)是一种黏蛋白样糖蛋白，由血管内皮细胞特异性表达。 静脉和毛细血管。我们的初步研究揭示了EMCN在调节血管内皮细胞生长因子诱导中的作用 血管内皮细胞在体外和体内的正常血管生成中起作用。利用小干扰RNA降低EMCN的表达 导致血管内皮生长因子刺激的血管内皮细胞增殖、迁移和体外血管形成减少，并损害 活体视网膜血管形成。此应用程序建议检验EMCN在以下方面发挥作用的假设 血管内皮生长因子诱导病理性新生血管及EMCN对血管内皮生长因子刺激功能的影响 调整它的信号。研究概述了EMCN在病理性血管生成中的作用。 视网膜和脉络膜血管异常生长的模型以及理解分子 体外系统研究EMCN对血管内皮细胞生长因子诱导内皮细胞功能影响的机制。 目标1的研究将检验EMCN在病理性眼球发育中的作用。 体内新生血管的形成。氧诱导视网膜新生血管模型和激光诱导的视网膜新生血管 脉络膜新生血管模型将与功能获得和丧失研究结合使用， 在ICAM2的控制下使用siRNA和慢病毒递送的shEMCN，ICAM2是一种内皮特异性的 启动子，以及ICAM2控制下的AdEMCN。EMCN击倒的影响和 病理性血管生长的过度表达将被量化。目标2建议阐明分子 EMCN调节血管内皮细胞功能的机制。研究使用 EMCN缺失突变体将被用来确定EMCN的作用是否在细胞表面介导。 EMCN在细胞表面的作用将通过检测其对血管内皮生长因子结合和 VEGFR2的稳定/内化。细胞内效应将集中在EMCN的可能作用上 磷酸化、EMCN对VEGFR2磷酸酶活性的影响以及潜在的鉴定 EMCN胞内结合伙伴。这些拟议研究的结果将使我们更好地了解 EMCN在正常和病理性血管生成中的作用及可能导致新的内皮细胞的发展 抑制血管生长的细胞特异性疗法。 好了！