Request for a Varian 600 MHz NMR Instrument

描述（由申请人提供）：本申请要求购买一台600 MHz核磁共振光谱仪，供伊利诺伊大学厄巴纳-香槟分校基因组生物学研究所（IGB）的多研究员使用。IGB拥有大量美国国立卫生研究院资助的研究小组，他们正在开发天然产物发现的新方法，这是美国国立卫生研究院路线图中特别指出的目标。研究人员正在开发新的方法来发现广泛的天然产物，从磷酸盐和多酮，到非核糖体肽，植物衍生的类黄酮，以及核糖体合成和翻译后修饰的肽。目前这些努力的瓶颈是质量有限的样品的结构测定。近年来，毛细管LC核磁共振已被证明为此类样品提供解决方案，只需5-20¿L的样品，并通过使用微线圈技术提供10倍的灵敏度，超过常规探针。本提案描述了将CapLC核磁共振与600 MHz核磁共振光谱仪结合使用的建议。在此申请中，要求为600 MHz核磁共振光谱仪提供资金，以容纳由IGB购买的CapLC核磁共振探针。还描述了用户在IGB的其他使用。

DESCRIPTION (provided by applicant): This application requests the purchase of a 600 MHz NMR spectrometer for multi-investigator use in the Institute for Genomic Biology (IGB) at the University of Illinois at Urbana-Champaign. The IGB houses a large number of NIH-funded research groups that are developing new methods for natural product discovery, a goal that is specifically noted in the NIH Roadmap. Researchers are developing new methodology to discover a wide range of natural products, from phosphonates and polyketides, to non-ribosomal peptides, plant-derived flavonoids, and ribosomally synthesized and post-translationally modified peptides. Current bottlenecks in these efforts are structural determination of mass-limited samples. Capillary LC NMR has been shown in recent years to provide a solution for such samples by requiring just 5-20 ¿L of sample and providing 10-fold increase in sensitivity over regular probes by using microcoil technology. This proposal describes the proposed use of CapLC NMR in combination with a 600 MHz NMR spectrometer. In this application funds are requested for a 600 MHz NMR spectrometer to house CapLC NMR probes that would be purchased by the IGB. Additional use by users at the IGB is also described. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a shared nuclear magnetic resonance instrument for use by eight different laboratories at the University of Illinois. The instrument will be used for the structure determination of a variety of natural products that have promise for human use as therapeutics, herbicides, or antifungal agents.