Request for a Varian 600 MHz NMR Instrument

描述（由申请人提供）：本申请要求购买600 MHz NMR光谱仪，供伊利诺伊大学厄巴纳-香槟分校基因组生物学研究所（IGB）的多名研究者使用。IGB容纳了大量NIH资助的研究小组，这些研究小组正在开发天然产物发现的新方法，这是NIH路线图中特别指出的目标。研究人员正在开发新的方法来发现广泛的天然产物，从膦酸酯和聚酮化合物，到非核糖体肽，植物衍生的黄酮类化合物，以及核糖体合成和后修饰的肽。目前这些努力的瓶颈是质量有限的样品的结构测定。近年来，毛细管LC NMR已被证明为此类样品提供了解决方案，只需5-20微升样品，并通过使用微线圈技术提供了比常规探针高10倍的灵敏度。本提案描述了CapLC NMR与600 MHz NMR光谱仪的拟议用途。在这一申请中，要求提供资金，以购买一台600 MHz核磁共振光谱仪，用于安装IGB将购买的CapLC核磁共振探头。还描述了用户在IGB的其他使用。 公共卫生相关性：本申请要求资金购买共享的核磁共振仪器，供伊利诺伊大学的八个不同实验室使用。该仪器将用于各种天然产物的结构测定，这些天然产物有望用于人类作为治疗剂，除草剂或抗真菌剂。

DESCRIPTION (provided by applicant): This application requests the purchase of a 600 MHz NMR spectrometer for multi-investigator use in the Institute for Genomic Biology (IGB) at the University of Illinois at Urbana-Champaign. The IGB houses a large number of NIH-funded research groups that are developing new methods for natural product discovery, a goal that is specifically noted in the NIH Roadmap. Researchers are developing new methodology to discover a wide range of natural products, from phosphonates and polyketides, to non-ribosomal peptides, plant-derived flavonoids, and ribosomally synthesized and post-translationally modified peptides. Current bottlenecks in these efforts are structural determination of mass-limited samples. Capillary LC NMR has been shown in recent years to provide a solution for such samples by requiring just 5-20 ¿L of sample and providing 10-fold increase in sensitivity over regular probes by using microcoil technology. This proposal describes the proposed use of CapLC NMR in combination with a 600 MHz NMR spectrometer. In this application funds are requested for a 600 MHz NMR spectrometer to house CapLC NMR probes that would be purchased by the IGB. Additional use by users at the IGB is also described. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a shared nuclear magnetic resonance instrument for use by eight different laboratories at the University of Illinois. The instrument will be used for the structure determination of a variety of natural products that have promise for human use as therapeutics, herbicides, or antifungal agents.