Request for a Varian 600 MHz NMR Instrument

描述(由申请人提供)：本申请请求购买一台600 MHz核磁共振波谱仪，用于伊利诺伊大学香槟分校基因组生物学研究所(IGB)的多研究者使用。IGB拥有大量由NIH资助的研究小组，这些小组正在开发天然产品发现的新方法，这一目标在NIH路线图中有明确的说明。研究人员正在开发新的方法来发现广泛的天然产物，从磷酸盐和聚酮，到非核糖体多肽、植物来源的类黄酮，以及核糖体合成和翻译后修饰的多肽。目前这些努力的瓶颈是对质量有限的样品的结构性确定。近年来，毛细管LC核磁共振已被证明为这类样品提供了一种解决方案，只需5-20？L样品，并通过使用微线圈技术提供比常规探针高10倍的灵敏度。该提案描述了将CapLC核磁共振与600 MHz核磁共振波谱仪结合使用的建议。在这项申请中，需要为一台600兆赫核磁共振光谱仪提供资金，以容纳将由国际标准委员会购买的CapLC核磁共振探测器。还描述了用户在IGB的其他使用情况。 公共卫生相关性：这个应用程序申请资金购买一个共享的核磁共振仪器，供伊利诺伊大学的八个不同实验室使用。该仪器将用于各种天然产品的结构测定，这些产品有望作为治疗药物、除草剂或抗真菌药物用于人类。

DESCRIPTION (provided by applicant): This application requests the purchase of a 600 MHz NMR spectrometer for multi-investigator use in the Institute for Genomic Biology (IGB) at the University of Illinois at Urbana-Champaign. The IGB houses a large number of NIH-funded research groups that are developing new methods for natural product discovery, a goal that is specifically noted in the NIH Roadmap. Researchers are developing new methodology to discover a wide range of natural products, from phosphonates and polyketides, to non-ribosomal peptides, plant-derived flavonoids, and ribosomally synthesized and post-translationally modified peptides. Current bottlenecks in these efforts are structural determination of mass-limited samples. Capillary LC NMR has been shown in recent years to provide a solution for such samples by requiring just 5-20 ¿L of sample and providing 10-fold increase in sensitivity over regular probes by using microcoil technology. This proposal describes the proposed use of CapLC NMR in combination with a 600 MHz NMR spectrometer. In this application funds are requested for a 600 MHz NMR spectrometer to house CapLC NMR probes that would be purchased by the IGB. Additional use by users at the IGB is also described. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a shared nuclear magnetic resonance instrument for use by eight different laboratories at the University of Illinois. The instrument will be used for the structure determination of a variety of natural products that have promise for human use as therapeutics, herbicides, or antifungal agents.