4800 Plus MALDI TOF/TOF Analyzer

4800 Plus MALDI TOF/TOF 分析仪

• 批准号: 7794726
• 负责人: WILFRED A. VAN DER DONK
• 金额: $ 39.54万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-07 至 2011-07-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794726
• 关键词: Anabolism; Arts; Catalysis; Data; Dendrimers; Development; Drug Delivery Systems; Funding; Health; Human; Illinois; Investigation; Laboratories; Mass Spectrum Analysis; Nucleic Acids; Pathway interactions; Peptide Antibiotics; Polysaccharides; Request for Applications; Research Personnel; Sampling; Scientist; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Technology; Time; Universities; Weight; antimicrobial peptide; biomedical scientist; genome database; improved; instrument; metalloenzyme; molecular recognition; public health relevance; research study; sensor; volatile organic compound

DESCRIPTION (provided by applicant): This application requests the purchase of a Matrix-Assisted Laser Desorption Ionization (MALDI)-TOF-TOF (Time-of-Flight) mass spectrometry instrument for multi-investigator use in the Mass Spectrometry Laboratory (MSL) at the University of Illinois at Urbana- Champaign. The MSL serves a large number of departments at the University of Illinois and also receives many samples from outside. The requested instrument has greatly improved mass accuracy compared to our current MALDI instrument, and can deliver routine MS-MS data unlike our current instrument. Access to the requested state-of-the- art technology would enable many critical experiments in NIH-funded laboratories at the University of Illinois that cannot be performed at present. In particular, it will greatly aid the investigation of the biosynthesis of peptide antibiotics, the structural elucidation of cyclic antimicrobial peptides discovered from genome databases, the development of dendrimers as drug delivery vehicles, the development of important new sensors for volatile organic compounds, and the understanding of nucleic acid catalysis and molecular recognition. In addition, the requested instrument will be used to better understand metalloenzymes that are key to human health as well as to elucidate glycan biosynthetic pathways. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a state-of-the-art instrument that can accurately determine the weight of large biomolecules. In addition, the instrument can fragment the molecules and analyze the fragments, thereby providing further information that is key for biomedical scientists. The requested shared instrument will provide data for more than 100 scientists at the University of Illinois.

描述（由申请人提供）： 本申请要求购买基质辅助激光解吸电离（MALDI）-飞行时间（TOF）质谱仪，供伊利诺伊大学厄巴纳-尚潘分校质谱实验室（MSL）的多名研究者使用。MSL为伊利诺伊大学的大量部门提供服务，并从外部接收许多样品。与我们目前的MALDI仪器相比，所要求的仪器大大提高了质量准确度，并且可以提供常规MS-MS数据。获得所要求的最先进的技术将使许多关键的实验在美国国立卫生研究院资助的实验室在伊利诺伊大学，目前不能进行。特别是，它将大大有助于肽抗生素的生物合成的调查，从基因组数据库中发现的环状抗菌肽的结构阐明，树枝状聚合物作为药物递送载体的发展，挥发性有机化合物的重要新传感器的发展，以及核酸催化和分子识别的理解。此外，所要求的仪器将用于更好地了解对人类健康至关重要的金属酶，以及阐明聚糖生物合成途径。 公共卫生关系：这项申请要求资金购买一种最先进的仪器，可以准确地确定大生物分子的重量。此外，该仪器可以将分子片段化并分析片段，从而提供对生物医学科学家至关重要的进一步信息。所要求的共享仪器将为伊利诺伊大学的100多名科学家提供数据。

项目成果

Structural determinants of macrocyclization in substrate-controlled lanthipeptide biosynthetic pathways.

• DOI: 10.1039/d0sc01651a
• 发表时间: 2020-06-25
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Bobeica SC;Zhu L;Acedo JZ;Tang W;van der Donk WA
• 通讯作者: van der Donk WA

Peptide microarrays for the discovery of bioactive surfaces that guide cellular processes: a single step azide-alkyne "click" chemistry approach.

• DOI: 10.1039/c4tb00375f
• 发表时间: 2014-06
• 期刊: Journal of materials chemistry. B
• 作者: Douglas Zhang;K. Kilian

Insights into methyltransferase specificity and bioactivity of derivatives of the antibiotic plantazolicin.

• DOI: 10.1021/cb501042a
• 发表时间: 2015-05-15
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: Hao Y;Saint-Vincent PMB;Sharma A;Mitchell DA;Nair SK
• 通讯作者: Nair SK

Improved production of class I lanthipeptides in Escherichia coli.

改善了大肠杆菌中I类植物肽的产生。

• DOI: 10.1039/d2sc06597e
• 发表时间: 2023-03-08
• 期刊: CHEMICAL SCIENCE
• 影响因子: 8.4
• 作者: Lee, Hyunji;Wu, Chunyu;Desormeaux, Emily K.;Sarksian, Raymond;van der Donk, Wilfred A.
• 通讯作者: van der Donk, Wilfred A.

The Antimicrobial Activity of the Glycocin Sublancin Is Dependent on an Active Phosphoenolpyruvate-Sugar Phosphotransferase System.

• DOI: 10.1021/acsinfecdis.1c00157
• 发表时间: 2021-08-13
• 期刊: ACS infectious diseases
• 影响因子: 5.3
• 作者: Biswas S;Wu C;van der Donk WA
• 通讯作者: van der Donk WA