Two-Photon microscope for in vivo imaging and electrophysiology.

描述(由申请人提供)：这是一个由8名NIH资助的主要研究人员组成的小组提出的一项提案，他们要求提供资金购买商业上可用的双光子显微镜系统，这种共享的仪器将允许进行高分辨率的深层组织成像，这是了解大脑结构和功能所必需的。所要求的仅双光子系统将提供纽约大学医学院神经科学界目前难以或不可能用传统成像方法获得的有价值的新信息。拟议的研究涵盖发育、细胞和系统神经生物学项目，包括：皮质成像(项目1和2)。项目3)、纹状体(项目4)和视网膜神经节神经元(项目5)，以研究它们的发育、调节和功能；监测健康和疾病中中枢神经系统Ranvier节点的形成(项目6)；以及检测活的小鼠皮质中突触的结构和功能变化(项目7-8)。新系统提供了两个关键优势，将对本提案中提出的所有项目产生革命性影响：1)该仪器允许在组织中进行深达400微米的成像；2)通过将其与电生理装置相结合，它将允许结合活体成像和电生理研究。该设备将安装在斯米洛神经科学研究所， 该设备靠近所有八个用户的实验室，并将在时间允许的情况下向纽约大学的研究界提供，该设备将得到长期的机构支持，以支付服务合同以及技术人员的部分工资。所要求的系统将极大地促进纽约大学医学院的神经科学研究，并将有助于了解神经回路在正常和病理条件下是如何组装和发挥作用的。 与公共健康相关：这是由美国国立卫生研究院资助的八名首席研究员提出的一项建议，该小组要求提供资金，购买双光子显微镜系统，用于活体研究大脑结构和功能。在新成像系统的帮助下，拟议的研究将有助于更好地了解神经回路是如何组装和修改的，以及它们在正常和病理条件下如何发挥作用。这些知识将对开发治疗各种脑部疾病的治疗策略至关重要。

DESCRIPTION (provided by applicant): This is a proposal from a group of eight NIH funded principal Investigators requesting funds to purchase a commercially available two-Photon microscope system, This .shared Instrument will permit high resolution deep tissue imaging essential for understanding the structure and function of the brain. The requested two-photon only system will provide valuable new Information difficult or impossible to obtain with the traditional Imaging approaches presently available to the neuroscience community at NYU medical school. The proposed studies cover developmental, cellular and system neurobiology projects including: Imaging cortical (projects 1 and 2). Hippocampal (project 3), striatal (project 4) and retinal ganglion neurons (project 5) to study their development, regulation and function; monitoring the formation of Nodes of Ranvier in the CNS In health and disease (project 6); and examining the structural and functional changes of synapses in the living mouse cortex (projects 7-8). The new system provides two key advantages that will be transformative for all the projects presented in this proposal: 1) The instrument permits Imaging up to 400 um deep In tissues and 2) By combining It with an electrophysiological setup It will allow for combined In vivo imaging and electrophysiological studies, The device will be housed In the Smilow Neuroscience Institute, a shared facility close to all eight users' laboratories and will also be available to the research community at New York University as time permits, This device will have long-term Institutional support to cover the service contracts as well as part of the technician's salary. The requested system will greatly facilitate neuroscience research at NYU School of Medicine and will help understand how neural circuits are assembled and function under normal and pathological conditions. PUBLIC HEALTH RELEVANCE: This is a proposal from a group of eight NIH funded principal investigator's requesting funds to purchase a two-photon microscope system for in vivo studies of brain structure and function. With the help of the new imaging system, the proposed studies will lead to a better understanding of how neural circuits are assembled and modified and how they function under normal and pathological conditions. Such knowledge will be critical for developing therapeutic strategies for the treatment of various brain diseases.