Two-Photon microscope for in vivo imaging and electrophysiology.

描述（由申请人提供）：这是一份由八名NIH资助的首席研究员组成的小组提出的提案，要求资金购买市售双光子显微镜系统。共享仪器将允许高分辨率的深层组织成像，这对理解大脑的结构和功能至关重要。所要求的双光子系统将提供有价值的新信息，这些信息很难或不可能通过纽约大学医学院神经科学界现有的传统成像方法获得。建议的研究涵盖发育、细胞和系统神经生物学项目，包括：皮层成像（项目1和2）。海马神经元（项目3）、纹状体神经元（项目4）和视网膜神经节神经元（项目5）研究它们的发育、调控和功能；监测中枢神经系统朗维耶淋巴结的形成在健康和疾病（项目6）；并检查活体小鼠皮层突触的结构和功能变化（项目7-8）。新系统提供了两个关键优势，这将对本提案中提出的所有项目具有变革性：1)该仪器允许在组织中进行高达400微米的成像；2)通过将其与电生理装置相结合，它将允许结合体内成像和电生理研究

DESCRIPTION (provided by applicant): This is a proposal from a group of eight NIH funded principal Investigators requesting funds to purchase a commercially available two-Photon microscope system, This .shared Instrument will permit high resolution deep tissue imaging essential for understanding the structure and function of the brain. The requested two-photon only system will provide valuable new Information difficult or impossible to obtain with the traditional Imaging approaches presently available to the neuroscience community at NYU medical school. The proposed studies cover developmental, cellular and system neurobiology projects including: Imaging cortical (projects 1 and 2). Hippocampal (project 3), striatal (project 4) and retinal ganglion neurons (project 5) to study their development, regulation and function; monitoring the formation of Nodes of Ranvier in the CNS In health and disease (project 6); and examining the structural and functional changes of synapses in the living mouse cortex (projects 7-8). The new system provides two key advantages that will be transformative for all the projects presented in this proposal: 1) The instrument permits Imaging up to 400 um deep In tissues and 2) By combining It with an electrophysiological setup It will allow for combined In vivo imaging and electrophysiological studies, The device will be housed In the Smilow Neuroscience Institute, a shared facility close to all eight users' laboratories and will also be available to the research community at New York University as time permits, This device will have long-term Institutional support to cover the service contracts as well as part of the technician's salary. The requested system will greatly facilitate neuroscience research at NYU School of Medicine and will help understand how neural circuits are assembled and function under normal and pathological conditions. PUBLIC HEALTH RELEVANCE: This is a proposal from a group of eight NIH funded principal investigator's requesting funds to purchase a two-photon microscope system for in vivo studies of brain structure and function. With the help of the new imaging system, the proposed studies will lead to a better understanding of how neural circuits are assembled and modified and how they function under normal and pathological conditions. Such knowledge will be critical for developing therapeutic strategies for the treatment of various brain diseases.