Two-Photon microscope for in vivo imaging and electrophysiology.
用于体内成像和电生理学的双光子显微镜。
基本信息
- 批准号:8052334
- 负责人:
- 金额:$ 50万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-04-01 至 2012-03-31
- 项目状态:已结题
- 来源:
- 关键词:BrainCommunitiesContract ServicesCorpus striatum structureDevelopmentDevicesDiseaseElectrophysiology (science)FundingHealthHippocampus (Brain)HousingImageInstitutesLaboratoriesLifeMicroscopeMonitorMusNeurobiologyNeuronsNeurosciencesNeurosciences ResearchNew YorkPrincipal InvestigatorRanvier&aposs NodesRegulationResearchResolutionRetinal Ganglion CellsStructureSynapsesSystemTimeTissuesUnited States National Institutes of HealthUniversitiesWagesin vivoinstrumentmedical schoolsneural circuittwo-photon
项目摘要
DESCRIPTION (provided by applicant): This is a proposal from a group of eight NIH funded principal Investigators requesting funds to purchase a commercially available two-Photon microscope system, This .shared Instrument will permit high resolution deep tissue imaging essential for understanding the structure and function of the brain. The requested two-photon only system will provide valuable new Information difficult or impossible to obtain with the traditional Imaging approaches presently available to the neuroscience community at NYU medical school. The proposed studies cover developmental, cellular and system neurobiology projects including: Imaging cortical (projects 1 and 2). Hippocampal (project 3), striatal (project 4) and retinal ganglion neurons (project 5) to study their development, regulation and function; monitoring the formation of Nodes of Ranvier in the CNS In health and disease (project 6); and examining the structural and functional changes of synapses in the living mouse cortex (projects 7-8). The new system provides two key advantages that will be transformative for all the projects presented in this proposal: 1) The instrument permits Imaging up to 400 um deep In tissues and 2) By combining It with an electrophysiological setup It will allow for combined In vivo imaging and electrophysiological studies, The device will be housed In the Smilow Neuroscience Institute, a
shared facility close to all eight users' laboratories and will also be available to the research community at New York University as time permits, This device will have long-term Institutional support to cover the service contracts as well as part of the technician's salary. The requested system will greatly facilitate neuroscience research at NYU School of Medicine and will help understand how neural circuits are assembled and function under normal and pathological conditions.
PUBLIC HEALTH RELEVANCE: This is a proposal from a group of eight NIH funded principal investigator's requesting funds to purchase a two-photon microscope system for in vivo studies of brain structure and function. With the help of the new imaging system, the proposed studies will lead to a better understanding of how neural circuits are assembled and modified and how they function under normal and pathological conditions. Such knowledge will be critical for developing therapeutic strategies for the treatment of various brain diseases.
描述(由申请人提供):这是一份由八名NIH资助的主要研究者提出的提案,要求提供资金购买市售双光子显微镜系统,该共享仪器将允许进行高分辨率深层组织成像,这对于理解大脑的结构和功能至关重要。所要求的双光子系统将提供有价值的新信息,这些信息很难或不可能通过纽约大学医学院神经科学界目前可用的传统成像方法获得。拟议的研究涵盖发育,细胞和系统神经生物学项目,包括:成像皮层(项目1和2)。海马神经元(项目3)、纹状体神经元(项目4)和视网膜神经节神经元(项目5),以研究它们的发育、调节和功能;监测健康和疾病时中枢神经系统中朗维尔结的形成(项目6);以及检查活体小鼠皮层中突触的结构和功能变化(项目7-8)。新系统提供了两个关键优势,这将是本提案中提出的所有项目的变革:1)该仪器允许成像高达400 um深的组织和2)通过将其与电生理设置相结合,它将允许组合体内成像和电生理研究,该设备将被安置在Smilow神经科学研究所,
共享设施,靠近所有八个用户的实验室,并将提供给研究界在纽约大学的时间允许,该设备将有长期的机构支持,以支付服务合同以及部分技术人员的工资。所要求的系统将极大地促进纽约大学医学院的神经科学研究,并将帮助了解神经回路在正常和病理条件下如何组装和运作。
公共卫生关系:这是一个由八个NIH资助的首席研究员组成的小组提出的建议,要求资金购买一个双光子显微镜系统,用于大脑结构和功能的体内研究。在新成像系统的帮助下,拟议的研究将更好地了解神经回路是如何组装和修改的,以及它们在正常和病理条件下如何发挥作用。这些知识对于开发治疗各种脑部疾病的治疗策略至关重要。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WENBIAO GAN其他文献
WENBIAO GAN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WENBIAO GAN', 18)}}的其他基金
Mechanisms and therapeutics of calcium dysregulation and synapse loss in Alzheimer's disease
阿尔茨海默病钙失调和突触损失的机制和治疗
- 批准号:
9808603 - 财政年份:2019
- 资助金额:
$ 50万 - 项目类别:
Optogenetic signaling inhibitors for studying brain plasticity
用于研究大脑可塑性的光遗传学信号抑制剂
- 批准号:
9353464 - 财政年份:2016
- 资助金额:
$ 50万 - 项目类别:
LIPS: A novel technology for spatial and temporal control of protein synthesis in dendritic spines
LIPS:一种用于树突棘蛋白质合成时空控制的新技术
- 批准号:
9037179 - 财政年份:2015
- 资助金额:
$ 50万 - 项目类别:
LIPS: A novel technology for spatial and temporal control of protein synthesis in dendritic spines
LIPS:一种用于树突棘蛋白质合成时空控制的新技术
- 批准号:
9147633 - 财政年份:2015
- 资助金额:
$ 50万 - 项目类别:
In vivo studies of microglial functions in brain plasticity and pathology
小胶质细胞在脑可塑性和病理学中的功能的体内研究
- 批准号:
8674876 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
In vivo studies of microglial functions in brain plasticity and pathology
小胶质细胞在脑可塑性和病理学中的功能的体内研究
- 批准号:
8997545 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
In vivo studies of microglial functions in brain plasticity and pathology
小胶质细胞在脑可塑性和病理学中的功能的体内研究
- 批准号:
8792262 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
In vivo studies of microglial functions in brain plasticity and pathology
小胶质细胞在脑可塑性和病理学中的功能的体内研究
- 批准号:
9223747 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
Experience-dependent plasticity of synaptic structure.
突触结构的经验依赖性可塑性。
- 批准号:
8676596 - 财政年份:2013
- 资助金额:
$ 50万 - 项目类别:
Experience-dependent plasticity of synaptic structure
突触结构的经验依赖性可塑性
- 批准号:
8251352 - 财政年份:2011
- 资助金额:
$ 50万 - 项目类别:
相似海外基金
Improving Resilience of MCDI for Water Supply in Remote Communities
提高偏远社区供水的 MCDI 弹性
- 批准号:
DP240101469 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Discovery Projects
Reconnecting Warlpiri communities with cultural heritage materials
重新连接瓦尔皮里社区与文化遗产材料
- 批准号:
LP220200211 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Linkage Projects
An interdisciplinary analytical framework for high-mountain landslides and cascading hazards: implications for communities and infrastructure
高山滑坡和级联灾害的跨学科分析框架:对社区和基础设施的影响
- 批准号:
NE/Z503502/1 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Research Grant
Understanding Material Interactions and Effects on Polymicrobial Communities at Surfaces
了解材料相互作用和对表面多种微生物群落的影响
- 批准号:
BB/Y512412/1 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Training Grant
RII Track-1: Interface of Change: Building Collaborations to Assess Harvested and Farmed Marine Species Prioritized by Gulf of Alaska Communities Facing Environmental Shifts
RII Track-1:变革界面:建立合作来评估面临环境变化的阿拉斯加湾社区优先考虑的捕捞和养殖海洋物种
- 批准号:
2344553 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Cooperative Agreement
CAREER: Evaluating Cooperative Intelligence in Connected Communities
职业:评估互联社区中的合作智能
- 批准号:
2339497 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Continuing Grant
Creating cosmopolitan rural communities through Japanese crafts: a comparative perspective with Portugal and Brazil
通过日本手工艺创建国际化农村社区:与葡萄牙和巴西的比较视角
- 批准号:
24K21000 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Conference: Global Bioinformatics Education Summit 2024 — Energizing Communities to Power the Bioeconomy Workforce
会议:2024 年全球生物信息学教育峰会 — 激励社区为生物经济劳动力提供动力
- 批准号:
2421267 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
Doctoral Dissertation Research: Spatial and Geological Mapping in Local Communities
博士论文研究:当地社区的空间和地质测绘
- 批准号:
2342887 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
Building Capacity through Professional Learning Communities to Advance Identity Integration in STEM Pre-Service Teacher Preparation
通过专业学习社区进行能力建设,促进 STEM 职前教师准备中的身份整合
- 批准号:
2345042 - 财政年份:2024
- 资助金额:
$ 50万 - 项目类别:
Standard Grant