Request for Spinning Disc Confocal Microscopy System

描述（由申请人提供）：本申请是针对具有光漂白后荧光恢复（FRAP）和全内反射荧光（TIRF）能力的马里亚纳旋转圆盘共聚焦显微镜。National Jewish Health没有能够执行该仪器将提供的最先进图像分析的共聚焦显微镜。因此，对所请求的系统存在实质性需求。先进的成像技术已成为研究细胞和组织动态过程的重要工具。这些过程是理解宿主细胞的基础：病原体相互作用，凋亡细胞缺失机制，参与先天性和适应性免疫的细胞反应，肺细胞对损伤的反应，产生炎症介质和内吞运输的信号转导过程。这些例子说明了旋转圆盘共聚焦显微镜将如何用于支持国家犹太健康研究人员的NIH资助的生物医学研究。作为一个高度集中的生物医学研究机构，该仪器将提供尖端技术，以阐明呼吸道，传染病和过敏性疾病的内在过程。显微镜将存放在National Jewish Health的流式细胞术和显微镜核心设施中。最近翻新的核心设施由一名主任和两名技术人员管理，他们承诺从该机构获得支持。已经制定了一项切实可行的财务和管理计划，以确保该工具得到适当维护和有效使用。 公共卫生相关性：使用转盘共聚焦显微镜系统的研究将确定国家犹太健康研究的疾病过程中的新机制，包括哮喘，炎症和自身免疫。为了使研究人员在理解细胞和组织中的生理和病理过程方面取得突破，必须拥有最先进的分析设备，以生成高影响力的数据。

DESCRIPTION (provided by applicant): This application is for a Marianas Spinning Disk Confocal Microscope with capability of Fluorescence Recovery after Photo bleaching (FRAP) and Total Internal Refection Fluorescence (TIRF). National Jewish Health does not have a confocal microscope capable of performing state-of-the-art image analysis that this instrument will provide. Thus there is substantial need for the requested system. Sophisticated imaging technology has become an important tool for studying dynamic processes in cells and tissues. These processes are fundamental to understanding host cell: pathogen interactions, mechanisms of apoptotic cell deletion, responses of cells involved in innate and adaptive immunity, lung cell responses to injury, signal transduction processes for production of mediators of inflammation and endocytic trafficking. These examples illustrate how the spinning disk confocal microscope will be used to support the NIH-funded biomedical research by investigators at National Jewish Health. As a highly focused biomedical research institution, the instrument will provide cutting-edge technology to elucidate processes inherent in respiratory, infectious and allergic diseases. The microscope will be housed in the combined Flow Cytometry and Microscopy Core Facility at National Jewish Health. The recently renovated core facility is managed by a director and 2 technicians, who have committed support from the institution. A practical financial and management plan has been developed to ensure that the instrument will be properly maintained and effectively used. PUBLIC HEALTH RELEVANCE: Research from the use of the Spinning Disk Confocal Microscopy system will identify new mechanisms in disease processes studied at National Jewish Health including asthma, inflammation and autoimmunity. For investigators to make breakthroughs in understanding physiological and pathological processes in cells and tissues, it is essential to have state-of-the-art equipment for analysis to generate high-impact data.