EARLY INNATE IMMUNE RESPONSE IN DENGUE VIRUS INFECTION

登革热病毒感染的早期先天免疫反应

• 批准号: 8357451
• 负责人: GUEY-CHUEN PERNG
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357451
• 关键词: Acute; Blood Coagulation Disorders; Blood Platelets; Coagulation Process; Dendritic Cells; Dengue; Dengue Virus; Event; Funding; Goals; Grant; Human; Immune response; In Vitro; Infection; Macaca mulatta; Megakaryocytes; Modeling; National Center for Research Resources; Pathology; Phase; Primates; Principal Investigator; Protocols documentation; Research; Research Infrastructure; Resources; Role; Source; United States National Institutes of Health; Virus Diseases; cost; in vivo; macrophage; man

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The aims of this protocol are to document the early dissemination and pathology of DENGUE virus infection in rhesus macaques. Human infection with Dengue virus is generally detected about one week post infection and there is therefore a dearth of information on the early event of virus infection in a model close to man. This is the first instance in which coagulopathies have been induced in rhesus macaques following infection with Dengue virus infection. The goals of the study are 1) to determine the mechanism(s) in the coagulation cascade that is impaired by Dengue infection in vivo; b) the exact target of Dengue virus with a particular focus on megakaryocytes and platelets which appear susceptible in vitro and in vivo; c) the role of dendritic cells and macrophage in the acute phase of infection in vivo.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本方案旨在记录恒河猴中登革病毒感染的早期传播和病理学。人类感染登革病毒通常在感染后一周左右被检测到，因此在接近人类的模型中缺乏关于病毒感染早期事件的信息。这是恒河猴感染登革病毒后诱导凝血功能障碍的第一例。本研究的目的是1）确定体内登革热感染损害的凝血级联中的机制; B）登革热病毒的确切靶点，特别关注在体外和体内表现出易感性的巨核细胞和血小板; c）树突状细胞和巨噬细胞在体内感染急性期的作用。