One-Step In Situ RNA Integrity Analysis for FFPE Tissue

FFPE 组织的一步原位 RNA 完整性分析

• 批准号: 8200295
• 负责人: Chang Hee Kim
• 金额: $ 16.7万
• 依托单位: DXTERITY DIAGNOSTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-13 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8200295
• 关键词: Archives; Binding; Biological Assay; Biological Markers; Cancer Detection; Cancer Patient; Clinical; Clinical Pathology; Clinical Research; Cost Savings; Data; Detection; Development; Fluorescence; Formalin; Gene Expression; General Population; Genes; Goals; Housing; In Situ; In Vitro; Length; Ligation; Marketing; Measures; Metric; Molecular; Molecular Analysis; One-Step dentin bonding system; Paraffin Embedding; Pathology; Performance; Pharmaceutical Preparations; Phase; Process; Protocols documentation; Public Health; Quality Control; RNA; RNA Degradation; RNA Probes; Research; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Scientist; Signal Transduction; Slide; System; Testing; Time; Tissue Microarray; Tissues; Transcript; assay development; cancer therapy; cost; fluorescence microscope; improved; outcome forecast

DESCRIPTION (provided by applicant): A rapid and simple in situ test for RNA integrity in Formalin-Fixed Paraffin Embedded (FFPE) will be developed in order to facilitate the selection of well-annotated, archival FFPE clinical samples with high quality RNA for gene expression analysis. The ratio of fluorescence signal from Quenched Auto Ligation (QUAL) probes of different lengths will be used to assess the degree of RNA fragmentation in FFPE samples in situ, prior to cumbersome and time- consuming RNA extraction. The Quality Control (QC) metric from the QUAL probes will be correlated to the quality of downstream gene expression analysis by RT-PCR and microarray on RNA extracted from the FFPE samples assayed for RNA integrity. The eventual goal is to develop and commercialize a QC kit for FFPE sample RNA quality. This research will aid and accelerate the discovery of more accurate molecular biomarkers for detection, prognosis and treatment of cancer. PUBLIC HEALTH RELEVANCE: "One Step In Situ RNA Integrity Analysis for FFPE Tissue". There are millions of potentially valuable, well-annotated clinical pathology samples around the world many of which cannot be used for clinical studies because of poor sample quality. The proposed research will enable more accurate detection, prognosis and treatment of cancer by improving the accuracy of selecting only high quality archival pathology samples for molecular analysis. The development of a simple and rapid, commercially available test to assess the quality of a pathology sample will also accelerate the pace of molecular studies needed for the discovery of drugs for cancer patients, and the detection of cancer in the general population in order to improve public health.

描述（由申请人提供）：将开发一种快速、简单的原位检测福尔马林固定石蜡包埋（FFPE）中RNA完整性的方法，以方便选择带有高质量RNA的注释良好的存档FFPE临床样品进行基因表达分析。不同长度QUAL探针的荧光信号比例将用于评估FFPE样品中RNA的原位断裂程度，然后进行繁琐且耗时的RNA提取。QUAL探针的质量控制（QC）指标将与通过RT-PCR和芯片对FFPE样品中提取的RNA进行下游基因表达分析的质量相关，以检测RNA的完整性。最终目标是开发和商业化FFPE样品RNA质量的QC试剂盒。这项研究将有助于并加速发现更准确的分子生物标志物，用于癌症的检测、预后和治疗。