Electrical System for Rapid Bacterial Cultures

描述(由申请人提供)：拟议项目“快速细菌培养的电气系统”的广泛、长期目标是开发一种自动电阻抗分析仪，用于测量受污染血液中的细菌生长。该系统的目的是将诊断脓毒症的时间从1-2天缩短到几个小时。该项目的具体目标是(1)构建一种可每10-15分钟自动采样的搅拌培养系统，(2)构建执行所需电气(阻抗)测量的专用电子组件，以及(3)结合这些设备来测试系统自动快速测量血液样本中细菌生长的能力。拟议的系统一次只能支持一种培养，而临床实验室将要求同时分析10‘S的样本；因此，最终的具体目标将是(4)为能够同时测量多个血液样本培养中的细菌生长的临床版本的系统准备设计。这项研究的结果预计将大大减少在临床实验室中识别败血症和败血症微生物所需的时间(4-10倍)，从而每年避免数百例以医院为基础的死亡。 公共卫生相关性：住院患者通常害怕发生败血症(血液细菌感染)，这往往是致命的。脓毒症恢复的一个重要因素是疾病状态被正确识别的速度。密苏里大学的Techshot和他的同事提出了一种电子测量设备，可以在几个小时内检测到血液中的细菌生长，而不是目前的1到2天(这有时已经太晚了)。

DESCRIPTION (provided by applicant): The broad, long-term objective of the proposed project "Electrical System for Rapid Bacterial Cultures" is to develop an automated electrical impedance analyzer for measuring bacterial growth in contaminated blood. The purpose of the System is to hasten the time to diagnose sepsis from 1-2 days down to a few hours. The specific aims of the project are to (1) build an agitated culture system that can be sampled automatically every 10-15 minutes, (2) build a dedicated electronics package that performs the desired electrical (impedance) measurements, and (3) combine these devices to test the System's ability to automatically and rapidly measure bacterial growth in blood samples. The proposed System will accommodate only one culture at a time, whereas clinical laboratories will require that 10's of samples be analyzed simultaneously; therefore, the final specific aim will be to (4) prepare a design for a clinical version of the System capable of simultaneously measuring bacterial growth in multiple blood sample cultures. The outcome of this research is expected to be a drastically reduced time (factor of 4 - 10) required to identify sepsis and sepsis organisms in the clinical laboratory thereby averting hundreds of hospital-based deaths annually. PUBLIC HEALTH RELEVANCE: Hospitalized patients often fear an incident of sepsis (bacterial infection of the blood), which is too frequently fatal. A significant factor in sepsis recovery is the rate at which the disease state can be properly identified. Techshot and colleagues at the University of Missouri have proposed an electrical measurement device that can detect bacterial growth in blood in a few hours, rather than the 1 or 2 days currently (which is sometimes too late).