Electrical System for Rapid Bacterial Cultures

描述（由申请人提供）：拟议项目“快速细菌培养电气系统”的广泛、长期目标是开发一种自动电阻抗分析仪，用于测量污染血液中的细菌生长。该系统的目的是将脓毒症的诊断时间从1-2天缩短到几个小时。该项目的具体目标是：（1）建立一个搅拌培养系统，可以自动采样每10-15分钟，（2）建立一个专用的电子包，执行所需的电（阻抗）测量，和（3）联合收割机这些设备来测试系统的能力，自动和快速测量血液样品中的细菌生长。申报系统一次只能容纳一个培养物，而临床实验室要求同时分析10个样本;因此，最终的具体目标是（4）为能够同时测量多个血液样本培养物中细菌生长的系统临床版本准备设计。这项研究的结果预计将大大减少在临床实验室中识别脓毒症和脓毒症微生物所需的时间（4 - 10倍），从而每年避免数百例医院死亡。 公共卫生相关性：住院患者经常担心败血症（血液细菌感染）的事件，这通常是致命的。脓毒症恢复的一个重要因素是疾病状态可以被正确识别的速度。密苏里州大学的Techshot及其同事提出了一种电子测量设备，可以在几个小时内检测血液中的细菌生长，而不是目前的1或2天（有时为时已晚）。

DESCRIPTION (provided by applicant): The broad, long-term objective of the proposed project "Electrical System for Rapid Bacterial Cultures" is to develop an automated electrical impedance analyzer for measuring bacterial growth in contaminated blood. The purpose of the System is to hasten the time to diagnose sepsis from 1-2 days down to a few hours. The specific aims of the project are to (1) build an agitated culture system that can be sampled automatically every 10-15 minutes, (2) build a dedicated electronics package that performs the desired electrical (impedance) measurements, and (3) combine these devices to test the System's ability to automatically and rapidly measure bacterial growth in blood samples. The proposed System will accommodate only one culture at a time, whereas clinical laboratories will require that 10's of samples be analyzed simultaneously; therefore, the final specific aim will be to (4) prepare a design for a clinical version of the System capable of simultaneously measuring bacterial growth in multiple blood sample cultures. The outcome of this research is expected to be a drastically reduced time (factor of 4 - 10) required to identify sepsis and sepsis organisms in the clinical laboratory thereby averting hundreds of hospital-based deaths annually. PUBLIC HEALTH RELEVANCE: Hospitalized patients often fear an incident of sepsis (bacterial infection of the blood), which is too frequently fatal. A significant factor in sepsis recovery is the rate at which the disease state can be properly identified. Techshot and colleagues at the University of Missouri have proposed an electrical measurement device that can detect bacterial growth in blood in a few hours, rather than the 1 or 2 days currently (which is sometimes too late).