Biomarkers Core

Biomarker核心设施的目的是为以下方面提供全面的集中资源 评价软骨基质代谢所需的生物标志物分析及其作用 炎症在骨性关节炎发病机制中的作用该核心将服务于计划项目的所有项目 关于生物力学因素和炎症作用的共同兴趣而联系在一起的建议 在改善关节组织代谢及其对骨关节炎的贡献方面。提供的专业知识 通过这个核心设施，将能够对与软骨基质相关的因素进行分子分析 来自动物和人类样本的代谢和炎症，包括血清，滑液， 还有尿液。核心提供了从小样本体积测量多个分析物的能力 具有高灵敏度，这对于样品数量有限的项目特别有用。在……里面 此外，核心将协调与杜克·斯特德曼营养中心的合作，探索 代谢组学用于阐明项目1中高脂喂养小鼠的代谢特征。 信息将与从膝骨性关节炎患者获得的代谢图谱进行比较。 参与项目3，由杜克老龄中心资助。这一新颖而令人兴奋的方法将 对饮食、肥胖和骨关节炎之间的相互作用提供独特的见解。这一核心还将 为研究人员提供关于生物标记物分析原理和方法的培训资源。 我们预计这一核心将促进协作交互，并促进中央 与此办公自动化项目相关的假设。 与公共健康相关：这个生物标志物核心提供了对关节组织的最先进的分析 和炎症标志物。这些标记在所有项目中进行了测试，提供了独特的 有机会了解骨关节炎中肥胖和炎症的关系。

The purpose of the Biomarker Core facility is to provide a comprehensive, centralized resource for biomarker analyses required for the evaluation of cartilage matrix metabolism and the role of inflammation in the pathogenesis of OA. This Core will serve all of the projects of the Program Project proposal which are linked by a common interest in the role of biomechanical factors and inflammation in modifying joint tissue metabolism and their contribution to osteoarthritis. The expertise offered through this core facility will permit molecular analyses of factors associated with cartilage matrix metabolism and inflammation from both animal and human samples including serum, synovial fluid, and urine. The Core offers the capability of measuring multiple analytes from small sample volumes with high sensitivity, which is especially useful for projects involving limited quantities of samples. In addition, the Core will coordinate collaboration with the Duke Stedman Nutrition Center to explore the utility of metabolomics for elucidating metabolic profiles in the high fat fed mice in Project 1. This information will be compared with the metabolomic profiles obtained from patients with knee OA participating in Project 3, funded through the Duke Aging Center. This novel and exciting approach will provide unique insights into the interactions of diet, obesity, and osteoarthritis. This Core will also serve as a resource for the training of investigators on principles and methods of biomarker analyses. We anticipate that this Core will stimulate collaborative interaction and facilitate the testing of central hypotheses related to this OA Program Project. Relevance to Public Health: This Biomarkers Core provides state-of-the-art analyses of joint tissue and inflammation markers. These markers, tested across all of the projects, provide a unique opportunity to understand the relationship of obesity and inflammation in osteoarthritis.