Behavioral Core

行为核心

• 批准号: 8122318
• 负责人: PATRICIO T HUERTA
• 金额: $ 35.99万
• 依托单位: FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8122318
• 关键词: Affect; Amygdaloid structure; Animals; Antibodies; Area; Arts; Autoantibodies; Behavior; Behavior assessment; Behavioral; Behavioral Research; Binding; Biological Neural Networks; Brain; Brain region; Cognition; Cognitive; Complement; Computer software; Computers; Data; Data Analyses; Data Collection; Data Set; Data Storage and Retrieval; Dentistry; Diamond; Emotional; Ensure; Environment; Equipment; Event; Fetus; Functional disorder; Goals; Guidelines; Hippocampus (Brain); Housing; Human; Immunoglobulin G; Individual; Laboratories; Learning; Link; Lupus Erythematosus; Manuals; Measures; Medicine; Memory; Mus; N-Methyl-D-Aspartate Receptors; Neurologic; New Jersey; Outcome; Performance; Preparation; Process; Protocols documentation; Publications; Publishing; Reflex action; Reporting; Reproducibility of Results; Research; Research Personnel; Resources; Rest; Running; Syndrome; System; Technical Expertise; Testing; Time; Training; Universities; Veterinary Schools; behavior test; data exchange; design; experience; graduate student; in vivo; meetings; member; neuropsychiatry; programs; research study; skills; web site

Core B: Behavioral Core: (Huerta Director) This Core will enhance the individual projects in the Program by providing centralized resources for performing behavioral analysis in mice to test the main hypothesis that key syndromes of neuropsychiatric lupus erythematosus (NPSLE) are caused by autoantibodies that bind the NR2A and NR2B subunits of the N-methyl-D-aspartate receptor (NMDAR). Specifically, the research described in Project 1, Project 2 and Project 4 depends crucially on careful examination of the behavioral performance of mice. Mice will be subjected to a comprehensive battery of behavioral tests, designed to measure their neurological reflexes, sensorimotor skills, cognitive abilities, emotional processes, learning and memory; however, we will focus on the effects of anti-NMDAR antibodies on specific cognitive tasks that depend on the integrity of a specific NMDAR-rich brain area. The Behavioral Core will ensure that all the tests will be executed under similar environmental conditions and following the same protocol guidelines. This will permit a direct comparison of results across mouse groups tested for different autoantibodies on different times. Another clear advantage will be availability of automated tracking of animals and software-driven protocols, thus minimizing human error and subjective scoring. Finally, the data will be stored in a centralized fashion, with simple access to all investigators in the Program.

核心B：行为核心：（韦尔塔导演） 该核心将通过提供集中的资源来加强该计划中的各个项目 用于在小鼠中进行行为分析，以测试主要假设，即 神经精神性红斑狼疮（NPSLE）是由自身抗体引起的， N-甲基-D-天冬氨酸受体（NMDAR）的NR 2A和NR 2B亚基。具体而言是 项目1、项目2和项目4中描述的研究关键取决于仔细的 检查小鼠的行为表现。小鼠将接受全面的 一系列行为测试，旨在测量他们的神经反射，感觉运动技能， 认知能力，情感过程，学习和记忆;然而，我们将重点放在 抗NMDAR抗体对特定认知任务的影响，这些任务取决于大脑皮层的完整性。 特定的富含NMDAR的大脑区域。行为核心将确保所有测试都将 在类似的环境条件下并遵循相同的方案指南执行。 这将允许直接比较针对不同免疫原性测试的小鼠组之间的结果。 不同时间的自身抗体另一个明显的优势将是自动化的可用性， 跟踪动物和软件驱动的协议，从而最大限度地减少人为错误和主观 得分。最后，数据将以集中的方式存储，可以简单地访问所有 项目中的调查员。