ANALYTIC CYTOLOGY CORE FACILITY

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 NIH-RCMI外部研究中心评审员将分析细胞学核心设施评为优秀，并建议雇用一名额外的电子显微镜技术员并建立用户费用。外部顾问，E。Ann Ellis建议雇用一名经过认证的电子显微镜技术员，收取用户费用，并为蔡司EM-10购买一台高分辨率数码相机，以加快提供高质量的数码图像。通过在Zeiss Axioskop荧光显微镜或Zeiss Pascal共聚焦扫描显微镜中使用免疫荧光对独特蛋白质进行免疫定位，以及利用Zeiss EM-10透射电子显微镜进行免疫金标记，是翻译生物学、寄生虫及其独特分泌产物、病毒蛋白、细菌和真菌毒力因子的鉴定的高分辨率的基本技术，重金属和砷毒性机制、肿瘤鉴定、细胞信号分子以及代表生物科学、健康科学、化学、材料和冶金工程以及机械和工业工程的BBRC用户的许多其他技术。本提案规定协助设计方案，并协助使用细胞学分析核心设施中精密、保养良好和受到监督的设备。每台显微镜都配有图像分析、图像处理和数字图像打印软件。每台显微镜上都有服务合同。计算机和软件定期升级。定期举办共焦讲习班，使用户熟悉新技术和新协议。目前，有32个不同的项目利用这一设施。在过去几年中，使用这一设施的人数急剧增加。随着需要细胞微生物学设备的新教师的招聘，预计会有更多的使用。该设施提供必要的设备，以支持我们的生物科学博士课程的病理生物学重点，最终目标是通过科学家和工程师之间的跨学科合作，研究边境健康的研究生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The NIH-RCMI outside site reviewers rated the Analytical Cytology Core Facility Outstanding and recommended the hiring of an additional technician for electron microscopy and the establishment of users fees. The outside consultant, E. Ann Ellis recommended the hiring of a certified electron microscope technician, user fees and the purchase of a high resolution digital camera for the Zeiss EM-10 in order to expedite the provision of superb quality digital images. Immunolocalization of unique proteins by the use of immunofluorescence in the Zeiss Axioskop epifluorescence microscope or the Zeiss Pascal confocal scanning microscope and immunogold labeling utilizing the Zeiss EM-10 transmission electron microscope are essential techniques of high resolution for translational biology, the identification of parasites and their unique secretory products, viral proteins, bacterial and fungal virulence factors, mechanisms of heavy metal and arsenic toxicity, tumor identification, cell signaling molecules and many other techniques of the BBRC users who represent Biological Sciences, Health Sciences, Chemistry, Materials and Metallurgical Engineering and Mechanical and Industrial Engineering. This proposal provides for assistance in the designing of protocols and the use of sophisticated, well maintained and supervised equipment in the Analytical Cytology Core facility. Each microscope is equipped with software for image analysis, image processing and printing of digital images. Service contracts are maintained on each of the microscopes. Computers and software are regularly upgraded. Confocal workshops have been held regularly to familiarize users with new techniques and new protocols. Currently, there are thirty-two different projects utilizing this facility. In the last few years there has been a dramatic increase in the use of this facility. Greater use is anticipated with the hiring of new faculty who need cellular microbiology equipment. The facility provides essential equipment in support of the pathobiology emphasis of our doctoral program in Biological Sciences with the ultimate goal of research productivity in the study of border health by an interdisciplinary cooperation between scientists and engineers.