ANALYTIC CYTOLOGY CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 得克萨斯大学埃尔帕索分校提议继续支持分析细胞学核心设施，这是一个多用户研究设施，致力于协助教职员工和学生设计方案，使用蔡司EM-10透射式电子显微镜、蔡司Axioskop表面荧光显微镜、图像分析和图像处理，以及最近获得的带有冷冻台的徕卡Ultraut R超微切片机。这使我们能够使用冷冻免疫溶解技术。有16名教职员工和学生有利用该设施的项目。我们预计，这一核心设施将对我们向其提供病毒学和生理学职位的新教员至关重要。本实验室提供的其他设备包括一台带有闪点采集卡的Pulnix 1001模拟/数字相机，它使我们能够将数字图像发送到装有Image Pro软件的Dell Optiplex GX110计算机。这些图像可以在柯达染料升华打印机上打印。这为我们提供了一个数字暗室。佩尔科固定微波炉减少了固定所需的时间。索瓦尔临界点干燥设备用于另一个实验室使用扫描电子显微镜的项目。蔡司Axioskop配备了35 mm相机、Optronics数码相机、Optimas软件、Gateway计算机、惠普扫描仪、尼康35 mm扫描仪和Tektronix Phaser 740彩色激光打印机。还提供定制的暗室设施。该设施将配备一名主管/技术员，专职负责监督用户、监督和及时检查设备、准备化学解决方案、修理超微显微镜和暗室设备、维护设备日志、执行安全条例、处理废化学品、订购用品和设备、监督新设备的安装和维护库存。将向核心主任提供部分薪金，以便对这些活动进行持续监督。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Texas at El Paso proposes to continue support for the Analytical Cytology Core Facility, a multi-user research facility dedicated to assisting faculty, staff and students in the design of protocols, the use of the Zeiss EM-10 transmission electron microscope, the Zeiss Axioskop epifluorescence microscope, image analysis and image processing, as well as the recently acquired Leica Ultracut R ultramicrotome with a cryostage. This enables us to use cryoimmunolableling techniques. There are sixteen faculty whose staff and students have projects utilizing this facility. We anticipate that this core facility will be essential to new faculty to whom we have offered positions in virology and physiology. Additional equipment available in this laboratory includes a Pulnix 1001 analog/digital camera with a Flash point capture card which enables us to send digital images to a Dell Optiplex GX110 computer with Image Pro software. These images can be printed on a KODAK dye sublimation printer . This provides us with a digital darkroom. A Pelco fixation microwave reduces the amount of time needed for fixations. A Sorvall critical point drying apparatus is used for projects utilizing SEM in another laboratory. The Zeiss Axioskop is equipped with a 35 mm camera, an Optronics digital camera, OPTIMAS software, a Gateway computer, a Hewlett Packard scanner, a Nikon 35 mm scanner and a Tektronix Phaser 740 color laser printer. Custom darkroom facilities are also available. This facility will be staffed full-time by a supervisor/technician responsible for supervising users, oversight and timely inspection of equipment, preparing chemical solutions, repairing ultramicrotomes and darkroom equipment, maintaining log books for the equipment, enforcing safety regulations, disposing of waste chemicals, ordering supplies and equipment, supervising the installation of new equipment, and maintaining the inventory. Partial salary will be provided to the core director for ongoing oversight of these activiites.