BIOTECHNOGY: PATHWAYS TO DISEASE PREVENTION AND THERAPY (BPDPT)

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 摘要 生物技术：疾病预防和治疗的途径(BPDPT)是先前资助的分子生物学研究活动的扩展。其持续的使命是为大学生物医学研究人员提供当代分子生物学技术和技术专长。这将加强佛罗里达农工大学的研究基础设施。这项工作的长期目标是建立信息和技术资源，以增加研究人员的分子生物学专业知识，持续的研究人员合作，并培育新的生物医学研究合作项目。BPDPT的开发还将增加佛罗里达农工大学新的教师招聘机会。为了实现这些目标，已经确定了以下具体目标：1)建立分子生物学研究所需的设备基础设施；2)为生物医学研究人员建立一个大学范围内的合作环境；3)促进教师接受分子生物学培训的途径；以及4)增加参与分子生物学研究的研究人员的数量。目前已建立了实时荧光定量聚合酶链式反应、蛋白印迹分析、凝胶电泳法分析蛋白质、DNA、RNA、彗星试验、流式细胞术等传统分子生物学技术。在拟议的活动中，我们计划扩大分子生物学专业知识，以包括蛋白质组学和基因组学。最终，我们计划将分子生物学研究活动扩展到一个功能齐全、自给自足的核心设施。总体而言，BPDPT将有助于建立有益的资源，从而增加研究人员的分子生物学专业知识，持续的研究人员合作，并促进新的生物医学研究合作企业，并加强佛罗里达农工大学的研究基础设施。由NIH/NCRR/RCMI G12RR03020支持。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. ABSTRACT The Biotechnology: Pathways to Disease Prevention and Therapy (BPDPT) is an expansion of the previously funded molecular biology research activity. Its' continuing mission is to provide the university biomedical research investigators with contemporary molecular biology technology and technical expertise. This will strengthen the research infrastructure at Florida A&M University. The long-term goal of this effort is to establish informational and technical resources that will result in increased molecular biology expertise of the research faculty, continual investigator collaborations, and foster new biomedical research collaborative ventures. Development of the BPDPT will also increase new faculty recruitment opportunities at Florida A&M University. To complete these goals the following specific aims have been identified: 1) Establish the equipment infrastructure needed for molecular biology research; 2) Develop a University wide collaborative environment for biomedical research investigators; 3) Facilitate avenues by which faculty can receive molecular biology training; and 4) Increase the number of investigators involved in molecular biology research. Currently we have established traditional molecular biology technology including real-time PCR; western blot analysis; protein, DNA, & RNA analysis via gel electrophoresis; DNA damage analysis via the COMET assay, and flow cytometry. In the proposed activity we plan to expand the molecular biology expertise to include proteomics and genomics. Ultimately, we plan to expand the molecular biology research activity to a full functioning self-sustaining core facility. Overall, the BPDPT will serve to establish beneficial resources that will result in increased molecular biology expertise of the research faculty, continual investigator collaborations, and foster new biomedical research collaborative ventures and strengthens the research infrastructure at Florida A&M University. Supported by NIH/NCRR/RCMI G12RR03020.