INVESTIGATING TOXICOLOGY OF THIOL-CAPPED QUANTUM DOTS OF CADMIUM AND LEAD

硫醇封端量子点的毒理学研究 镉和铅的含量 建议开展研究以阐明硫醇封端的镉、镉、铅的毒理学和药代动力学 和体内的PbS量子点(QD)。这些量子点表现出明亮、光稳定和尺寸可调的特点。 荧光。此外，它们的超细颗粒(2-6 nm)与蛋白质具有尺寸上的相似性。因此， 它们是传统荧光染料在生物医学成像中作为光学探针的潜在替代品 和诊断，前提是与重金属(镉和铅)有关的安全问题得到解决。 用硫醇封顶是一种潜在的途径，可以使这些危险的量子点对生物医学来说是安全的 应用，但封顶量子点的安全性和药代动力学尚不清楚。为了可行，量子点 必须在体内稳定，并从体内完全消除。我们的假设是，在硫醇的作用下 量子点在用于治疗目的时并不是完全无害的，因为它们很容易与生物结合。 分子和生物会在体内积累。盖子会在体内被取下，导致暴露 对全身有害的量子点和重金属。为了阐明词汇学上的后果 暴露，大鼠将暴露于含硫醇的量子点(2-6 nm)静脉注射(IV)和不注射(IV) 用紫外光照射量子点。该项目的具体目标是：1)确定淘汰率 2)通过测量体内金属离子和量子点的含量来确定盖子的稳定性 尿液和血液；3)确定关键器官的蓄积限度，包括心脏、肝脏、肺、 肾脏、脑和骨骼；4)确定量子点的毒性作用和定位；5)统计学 评估与QD类型、剂量和紫外线相关的帽子稳定性、生物蓄积率和毒性效应 以及6)建立结构/毒性的计算模型来预测这些因素 对毒性的贡献最大。各种分析化学和毒物学方法将用于 结合紫外可见、等离子体源发射/质谱学和荧光显微镜 以确定器官中的量子点的特征。这个项目的结果将促进我们对 硫醇修饰的硫化镉、硫化镉、硒铅和硫化铅量子点的安全性和毒理学。圆满完成 该项目将为实现联合州立大学RCMI的总体目标做出巨大贡献，以增加高度 通过竞争培训环境健康和纳米毒理学研究方面的少数族裔专业人员 程序。

TOXICOLOGY OF THIOL-CAPPED QUANTUM DOTS OF CADMIUM AND LEAD Research is proposed to elucidate toxicology and pharmacokinetics of thiol-capped CdSe, CdS, PbSe and PbS quantum dots (QDs) in vivo. These QDs exhibit bright, photo-stable and size-tunable fluorescence. Further, their ultrafine particles (2-6 nm) show dimensional similarity with proteins. Thus, they are potential alternatives to traditional fluorescent dyes as optical probes in biomedical imaging and diagnostics, provided that the safety issues associated with heavy metals (Cd and Pb) are resolved. Capping with thiols is a potential avenue to render these hazardous QDs safe for biomedical applications, but the safety and pharmacokinetics of capped QDs are not known. To be viable, the QDs must be stable in vivo and eliminated completely from the body. Our hypothesis is that thiol-capped QDs will not be totally innocuous when used for therapeutic purposes as they readily bind to biological molecules and bioaccumulate in the body. The cap will be removed in vivo resulting in the exposure of the whole body to hazardous QDs and heavy metals. To elucidate the lexicological consequences of exposure, rats will be exposed to thiol-capped QDs (2-6 nm) intravenously (IV) with and without irradiating the QDs by UV light. The specific aims of the project are to: 1) determine the elimination rate of QDs from the body; 2) determine the cap stability by measuring content of metal ions and QDs in the urine and blood; 3) determine the accumulation limits in critical organs, including the heart, liver, lungs, kidney, brain and the bones; 4) determine the toxic effects and localization of QDs; 5) statistically evaluate cap stability, bioaccumulation profile and toxic effects associated with QD type, dose and UV light exposure; and 6) develop a computational model of structure/toxicity to predict the factors contributing most to toxicity. Various analytical chemistry and toxicology methods will be used in combination with UV-visible, plasma source emission/mass spectroscopy and fluorescence microscopy to characterize QDs in the organs. The results of this project will advance our understanding of the safety and toxicology of thiol-capped QDs of CdSe, CdS, PbSe and PbS. Successful completion of the project will contribute greatly to achieving the overall goal of RCMI at JSU to increase the pool of highly trained minority professionals in environmental health and nanotoxicology research through competitive programs.