INVESTIGATING TOXICOLOGY OF THIOL-CAPPED QUANTUM DOTS OF CADMIUM AND LEAD

研究镉和铅的硫醇封端量子点的毒理学

• 批准号: 8166140
• 负责人: Zikri Arslan
• 金额: $ 8.34万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166140
• 关键词: Acids; Alcohols; Animal Organ; Biological; Cadmium; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Digestion; Drug Kinetics; Ethanol; Exhibits; Funding; Grant; Hydroxide Ion; Hydroxides; Institution; Kidney; Lead; Light; Liver; Methods; Organ; Quantum Dots; Reporting; Research; Research Personnel; Resources; Route; Selenium; Signal Transduction; Solutions; Solvents; Sonication; Source; Sulfhydryl Compounds; Tissues; Toxicology; Ultrasonography; United States National Institutes of Health; Water; animal tissue; in vivo; insight; lead selenide; nanoparticle; particle; stability testing; tetramethylammonium; thioglycerol; visible spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Research is performed to elucidate toxicology and pharmacokinetics of thiol-capped CdSe, CdS, PbSe and PbS quantum dots (QDs) in vivo. Efforts have focused on the development of analytical and instrumental methods for as well synthesis of stable thiol-capped CdSe nanoparticles and their characterization. Two different routes were utilized for producing CdSe NPs in that we either used thioglycerol or mercaptoacetic acid for stabilizing the NPs. In either scenario, thiol-capped CdSe NPs were highly stable for months under ambient conditions. Purification studies were also completed. Characterization was performed with UV-visible spectrometry and TEM. Data indicated that size could be varied from 2-4 nm by varying the amount of accping agent or selenium. Stability tests were under sonication in different solvents. Particles were highly stable in water under ultrasounds, but exhibited instability in alcohol and acidic media resulting aggregation. Effect of the media was examined on the extraction of ionic Cd from animal tissues for speciation of ionic Cd and CdSe from the animal organs. Water and ethanol were unsuitable for extraction of Cd from biological tissues. Extraction achieved in acid media, however, the use of acid resulted in decomposition of CdSe NPs that introduced additional ionic Cd to solution. Tetramethylammonium hydroxide (TMAH) was found to be suitable for extraction. Extraction efficiency was ranged between 85-100% when testetd with liver reference material (DOLT-4). Treatment of CdSe NPs did not induce and degradation of Cd from NPs to solution ultrasounds, nor did cause any degradation during TMAH digestions. The differences in Cd signals from untreated and treated CdSe NP solutions were statistically insignificant. Preliminary data is reported to get an insight into the distribution of Cd and CdSe NPs in organs. Data indicated that majority of CdSe is in the form of ionic Cd in the organs (liver and kidney). Exposure of NPs to light induces enhancement on the levels of ionic Cd.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 开展研究以阐明硫醇封端的 CdSe、CdS、PbSe 和 PbS 量子点 (QD) 的体内毒理学和药代动力学。我们的工作重点是开发分析和仪器方法，以及合成稳定的硫醇封端的 CdSe 纳米颗粒及其表征。 采用两种不同的路线来生产 CdSe 纳米颗粒，即我们使用硫代甘油或巯基乙酸来稳定纳米颗粒。在这两种情况下，硫醇封端的 CdSe 纳米粒子在环境条件下数月内都高度稳定。纯化研究也已完成。通过紫外-可见光谱和 TEM 进行表征。数据表明，通过改变添加剂或硒的量，尺寸可以在2-4nm之间变化。 稳定性测试是在不同溶剂中进行超声处理。超声波下颗粒在水中高度稳定，但在酒精和酸性介质中表现出不稳定，导致聚集。检查了介质对从动物组织中提取离子 Cd 的影响，以了解动物器官中离子 Cd 和 CdSe 的形态。水和乙醇不适合从生物组织中提取Cd。然而，在酸性介质中进行萃取，使用酸会导致 CdSe NP 分解，从而向溶液中引入额外的离子 Cd。发现四甲基氢氧化铵 (TMAH) 适合萃取。用肝脏标准物质 (DOLT-4) 测试时，提取效率在 85-100% 之间。 CdSe NP 的处理不会诱导 Cd 从 NP 到溶液超声的降解，也不会在 TMAH 消化过程中引起任何降解。未经处理和处理的 CdSe NP 溶液的 Cd 信号差异在统计上不显着。据报道，初步数据可深入了解 Cd 和 CdSe 纳米粒子在器官中的分布。数据表明，大部分 CdSe 在器官（肝脏和肾脏）中以离子 Cd 的形式存在。纳米粒子暴露在光下会导致镉离子水平的增强。